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P59695

- TNFA_PAPAN

UniProt

P59695 - TNFA_PAPAN

Protein

Tumor necrosis factor

Gene

TNF

Organism
Papio anubis (Olive baboon)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (23 May 2003)
      Previous versions | rss
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    Functioni

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation By similarity.By similarity
    The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei39 – 402Cleavage; by SPPL2A or SPPL2BBy similarity
    Sitei49 – 502Cleavage; by SPPL2A or SPPL2BBy similarity
    Sitei51 – 522Cleavage; by SPPL2A or SPPL2BBy similarity
    Sitei76 – 772Cleavage; by ADAM17By similarity

    GO - Biological processi

    1. immune response Source: InterPro
    2. necroptotic signaling pathway Source: UniProtKB
    3. negative regulation of protein complex disassembly Source: UniProtKB
    4. positive regulation of apoptotic process Source: UniProtKB
    5. positive regulation of JUN kinase activity Source: UniProtKB
    6. positive regulation of MAP kinase activity Source: UniProtKB
    7. positive regulation of protein complex disassembly Source: UniProtKB
    8. positive regulation of protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tumor necrosis factor
    Alternative name(s):
    Cachectin
    TNF-alpha
    Tumor necrosis factor ligand superfamily member 2
    Short name:
    TNF-a
    Cleaved into the following 6 chains:
    Gene namesi
    Name:TNF
    Synonyms:TNFA, TNFSF2
    OrganismiPapio anubis (Olive baboon)
    Taxonomic identifieri9555 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaePapio

    Subcellular locationi

    Chain C-domain 1 : Secreted By similarity
    Chain C-domain 2 : Secreted By similarity

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233Tumor necrosis factor, membrane formPRO_0000034439Add
    BLAST
    Chaini1 – 3939Intracellular domain 1By similarityPRO_0000417263Add
    BLAST
    Chaini1 – 3535Intracellular domain 2By similarityPRO_0000417264Add
    BLAST
    Chaini50 – ?C-domain 1By similarityPRO_0000417265
    Chaini52 – ?C-domain 2By similarityPRO_0000417266
    Chaini77 – 233157Tumor necrosis factor, soluble formBy similarityPRO_0000034440Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Phosphoserine; by CK1By similarity
    Lipidationi20 – 201N6-myristoyl lysineBy similarity
    Glycosylationi80 – 801O-linked (GalNAc...); in soluble formBy similarity
    Disulfide bondi145 ↔ 177By similarity

    Post-translational modificationi

    The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space By similarity.By similarity
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1 By similarity.By similarity
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Myristate, Phosphoprotein

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with SPPL2B By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP59695.
    SMRiP59695. Positions 82-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini58 – 233176ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5723Helical; Signal-anchor for type II membrane proteinBy similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tumor necrosis factor family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG012516.

    Family and domain databases

    Gene3Di2.60.120.40. 1 hit.
    InterProiIPR006053. TNF.
    IPR002959. TNF_alpha.
    IPR021184. TNF_CS.
    IPR006052. TNF_dom.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view]
    PfamiPF00229. TNF. 1 hit.
    [Graphical view]
    PRINTSiPR01234. TNECROSISFCT.
    PR01235. TNFALPHA.
    SMARTiSM00207. TNF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49842. SSF49842. 1 hit.
    PROSITEiPS00251. TNF_1. 1 hit.
    PS50049. TNF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P59695-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTESMIRDV ELAEEALPRK TAGPQGSRRR WFLRLFSFLL VAGATTLFCL    50
    LHFGVIGPQR EEFPKDPSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG 100
    QLQWLNRRAN ALLANGVEPT DNQLVVPSEG LYLIYSQVLF KGQGCPSNHV 150
    LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF 200
    QLEKGDRLSA EINLPDYLDF AESGQVYFGI IAL 233
    Length:233
    Mass (Da):25,736
    Last modified:May 23, 2003 - v1
    Checksum:i0C477F9EB6CC9909
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY234222 mRNA. Translation: AAO85335.1.
    RefSeqiNP_001106118.1. NM_001112648.1.
    UniGeneiPan.11103.

    Genome annotation databases

    GeneIDi100126739.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY234222 mRNA. Translation: AAO85335.1 .
    RefSeqi NP_001106118.1. NM_001112648.1.
    UniGenei Pan.11103.

    3D structure databases

    ProteinModelPortali P59695.
    SMRi P59695. Positions 82-233.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100126739.

    Organism-specific databases

    CTDi 7124.

    Phylogenomic databases

    HOVERGENi HBG012516.

    Family and domain databases

    Gene3Di 2.60.120.40. 1 hit.
    InterProi IPR006053. TNF.
    IPR002959. TNF_alpha.
    IPR021184. TNF_CS.
    IPR006052. TNF_dom.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view ]
    Pfami PF00229. TNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01234. TNECROSISFCT.
    PR01235. TNFALPHA.
    SMARTi SM00207. TNF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49842. SSF49842. 1 hit.
    PROSITEi PS00251. TNF_1. 1 hit.
    PS50049. TNF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules."
      Villinger F.J., Bostik P., Mayne A.E., King C.L., Genain C.P., Weiss W.R., Ansari A.A.
      Immunogenetics 53:315-328(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiTNFA_PAPAN
    AccessioniPrimary (citable) accession number: P59695
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 23, 2003
    Last sequence update: May 23, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3