P59690 (SYE_CHLCV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CCA_00182

Organism

Chlamydophila caviae (strain GPIC) [Complete proteome] [HAMAP]

Taxonomic identifier

227941 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila › Chlamydophila caviae

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000119537
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	253 – 257	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	256	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59690 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: CC830B9332638305	FASTA	505	58,544
	10 20 30 40 50 60 MAWENVRVRV APSPTGDPHV GTAYMALFNE IFAKRFNGKM ILRIEDTDQT RSRDDYEKNI 70 80 90 100 110 120 FSALQWCGIQ WDEGPDIGGP HGPYRQSERT EIYREYAELL LKTDYAYKCF ATPKELEEMR 130 140 150 160 170 180 AVATTLGYRG GYDRRYRYLS PEEIEARTQE GQPYTIRLKV PLTGECVLED YCKGRVVFPW 190 200 210 220 230 240 ADVDDQVLMK SDGFPTYHFA NVVDDHLMGI THVLRGEEWL SSTPKHLLLY EAFGWEPPIF 250 260 270 280 290 300 LHMPLLLNPD GTKLSKRKNP TSIFYYRDAG YIKEAFMNFL TLMGYSMEGD EEVYSLEKLI 310 320 330 340 350 360 ANFDPKRIGK SGAVFDVRKL DWMNKHYLNH EGSPENLLAR LKDWLVNDEF LLKILPLCQS 370 380 390 400 410 420 RMATLAEFVG LSEFFFSVLP EYSKEELLPA AISQEKAAIL FYSYVKYLEK TDLWVKDQFY 430 440 450 460 470 480 LGSKWLSEAF QVHHKKVVIP LLYVAITGKK QGLPLFDSME LLGKPRTRMR MVHAQNLLGG 490 500 VPKKIQTAID KVLKEENFEN KILEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologs of Escherichia coli recJ, gltX and of a putative 'early' gene of avian Chlamydia psittaci are located upstream of the 'late' omp2 locus of Chlamydia psittaci strain guinea pig inclusion conjunctivitis." Hsia R.C., Bavoil P.M. Gene 176:163-169(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GPIC.
[2]	"Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae." Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G. , Rank R.G., Bavoil P.M., Fraser C.M. Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GPIC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41759 Genomic DNA. Translation: AAB41141.1. AE015925 Genomic DNA. Translation: AAP04933.1.
PIR	JC5208.
RefSeq	NP_829055.1. NC_003361.3.
3D structure databases
ProteinModelPortal	P59690.
ModBase	Search...
Protein-protein interaction databases
STRING	227941.CCA00182.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP04933; AAP04933; CCA_00182.
GeneID	1217867.
KEGG	cca:CCA00182.
PATRIC	20269380. VBIChlCav107360_0190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
KO	K01885.
OMA	WENVRVR.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CCAV227941:GH8L-189-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CHLCV

Accession

Primary (citable) accession number: P59690
Secondary accession number(s): P94662, Q06560

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program