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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (YALI0_F07711g)
  3. ATP-dependent 6-phosphofructokinase (PFK1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei193ATP; via amide nitrogenUniRule annotation1
Metal bindingi287Magnesium; catalyticUniRule annotation1
Active sitei334Proton acceptorUniRule annotation1
Binding sitei369Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei433SubstrateUniRule annotation1
Binding sitei460Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei645Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei740Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei807Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei833Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei906Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 257ATPUniRule annotation2
Nucleotide bindingi286 – 289ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFK1
Ordered Locus Names:YALI0D16357g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome D

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120441 – 953ATP-dependent 6-phosphofructokinaseAdd BLAST953

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP59680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 558N-terminal catalytic PFK domain 1Add BLAST558
Regioni332 – 334Substrate bindingUniRule annotation3
Regioni376 – 378Substrate bindingUniRule annotation3
Regioni466 – 469Substrate bindingUniRule annotation4
Regioni559 – 572Interdomain linkerAdd BLAST14
Regioni573 – 953C-terminal regulatory PFK domain 2Add BLAST381
Regioni702 – 706Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni747 – 749Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni839 – 842Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000200154.
InParanoidiP59680.
KOiK00850.
OMAiWLFIPER.
OrthoDBiEOG092C0LOE.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA
60 70 80 90 100
GSIQEVWLTS SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY
110 120 130 140 150
YAPDLDAVRA AITPFLHEDH STLLERPSHT NFIELYAIDP MGNLVGFSRR
160 170 180 190 200
ENPYSSAMQK PFSADDIGPQ NFSKPNETKI KGKKRIGVMT SGGDAPGMCA
210 220 230 240 250
AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED VRGWLSLGGT
260 270 280 290 300
LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW
310 320 330 340 350
PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL
360 370 380 390 400
DRICELVDFI DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE
410 420 430 440 450
AAPDATQWAE KMTRVVKRHR SQGKRKTVVI VAEGAIDSDL NPITAKMVKD
460 470 480 490 500
VLDGIGLDTR ISTLGHVQRG GPPVAADRVL ASLQGVEAID AILSLTPETP
510 520 530 540 550
SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR LRNPEFVEQL
560 570 580 590 600
QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR
610 620 630 640 650
GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC
660 670 680 690 700
DLGMIAYHFQ RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP
710 720 730 740 750
ATISNNVPGT DYSLGADTCL NSLVQYCDVL KTSASATRLR LFVVEVQGGN
760 770 780 790 800
SGYIATVAGL ITGAYVVYTP ESGINLRLLQ HDISYLKDTF AHQADVNRTG
810 820 830 840 850
KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV QQGGHPSPTD
860 870 880 890 900
RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK
910 920 930 940 950
TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII

DPC
Length:953
Mass (Da):103,753
Last modified:July 19, 2004 - v2
Checksum:i50DA57F8D774F74B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY142710 mRNA. Translation: AAN34943.2.
CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeqiXP_502897.1. XM_502897.1.

Genome annotation databases

EnsemblFungiiCAG81088; CAG81088; YALI0_D16357g.
GeneIDi2910306.
KEGGiyli:YALI0D16357g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY142710 mRNA. Translation: AAN34943.2.
CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeqiXP_502897.1. XM_502897.1.

3D structure databases

ProteinModelPortaliP59680.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG81088; CAG81088; YALI0_D16357g.
GeneIDi2910306.
KEGGiyli:YALI0D16357g.

Phylogenomic databases

HOGENOMiHOG000200154.
InParanoidiP59680.
KOiK00850.
OMAiWLFIPER.
OrthoDBiEOG092C0LOE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_YARLI
AccessioniPrimary (citable) accession number: P59680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.