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P59680

- PFKA_YARLI

UniProt

P59680 - PFKA_YARLI

Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei193 – 1931ATP; via amide nitrogenUniRule annotation
    Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
    Active sitei334 – 3341Proton acceptorUniRule annotation
    Binding sitei369 – 3691Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei433 – 4331SubstrateUniRule annotation
    Binding sitei460 – 4601Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei645 – 6451Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei740 – 7401Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei807 – 8071Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei906 – 9061Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi256 – 2572ATPUniRule annotation
    Nucleotide bindingi286 – 2894ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFK1
    Ordered Locus Names:YALI0D16357g
    OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
    Taxonomic identifieri284591 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
    ProteomesiUP000001300: Chromosome D

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953ATP-dependent 6-phosphofructokinasePRO_0000112044Add
    BLAST

    Interactioni

    Subunit structurei

    Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

    Protein-protein interaction databases

    STRINGi4952.P59680.

    Structurei

    3D structure databases

    ProteinModelPortaliP59680.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 558558N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni332 – 3343Substrate bindingUniRule annotation
    Regioni376 – 3783Substrate bindingUniRule annotation
    Regioni466 – 4694Substrate bindingUniRule annotation
    Regioni559 – 57214Interdomain linkerAdd
    BLAST
    Regioni573 – 953381C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000200154.
    KOiK00850.
    OMAiMIALNEN.
    OrthoDBiEOG7Q5HPV.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59680-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA    50
    GSIQEVWLTS SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY 100
    YAPDLDAVRA AITPFLHEDH STLLERPSHT NFIELYAIDP MGNLVGFSRR 150
    ENPYSSAMQK PFSADDIGPQ NFSKPNETKI KGKKRIGVMT SGGDAPGMCA 200
    AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED VRGWLSLGGT 250
    LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW 300
    PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL 350
    DRICELVDFI DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE 400
    AAPDATQWAE KMTRVVKRHR SQGKRKTVVI VAEGAIDSDL NPITAKMVKD 450
    VLDGIGLDTR ISTLGHVQRG GPPVAADRVL ASLQGVEAID AILSLTPETP 500
    SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR LRNPEFVEQL 550
    QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR 600
    GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC 650
    DLGMIAYHFQ RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP 700
    ATISNNVPGT DYSLGADTCL NSLVQYCDVL KTSASATRLR LFVVEVQGGN 750
    SGYIATVAGL ITGAYVVYTP ESGINLRLLQ HDISYLKDTF AHQADVNRTG 800
    KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV QQGGHPSPTD 850
    RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK 900
    TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII 950
    DPC 953
    Length:953
    Mass (Da):103,753
    Last modified:July 19, 2004 - v2
    Checksum:i50DA57F8D774F74B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY142710 mRNA. Translation: AAN34943.2.
    CR382130 Genomic DNA. Translation: CAG81088.1.
    RefSeqiXP_502897.1. XM_502897.1.

    Genome annotation databases

    EnsemblFungiiCAG81088; CAG81088; YALI0_D16357g.
    GeneIDi2910306.
    KEGGiyli:YALI0D16357g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY142710 mRNA. Translation: AAN34943.2 .
    CR382130 Genomic DNA. Translation: CAG81088.1 .
    RefSeqi XP_502897.1. XM_502897.1.

    3D structure databases

    ProteinModelPortali P59680.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4952.P59680.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAG81088 ; CAG81088 ; YALI0_D16357g .
    GeneIDi 2910306.
    KEGGi yli:YALI0D16357g.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000200154.
    KOi K00850.
    OMAi MIALNEN.
    OrthoDBi EOG7Q5HPV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The phosphofructokinase from the yeast Yarrowia lipolytica."
      Flores C.L., Martinez-Costa O.H., Sanchez V., Aragon J.J., Gancedo C.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CLIB 122 / E 150.

    Entry informationi

    Entry nameiPFKA_YARLI
    AccessioniPrimary (citable) accession number: P59680
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3