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P59680

- PFKA_YARLI

UniProt

P59680 - PFKA_YARLI

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Protein

ATP-dependent 6-phosphofructokinase

Gene
PFK1, YALI0D16357g
Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei193 – 1931ATP; via amide nitrogen By similarity
Metal bindingi287 – 2871Magnesium; catalytic By similarity
Active sitei334 – 3341Proton acceptor By similarity
Binding sitei369 – 3691Substrate; shared with dimeric partner By similarity
Binding sitei433 – 4331Substrate By similarity
Binding sitei460 – 4601Substrate; shared with dimeric partner By similarity
Binding sitei645 – 6451Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei740 – 7401Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei807 – 8071Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei906 – 9061Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2572ATP By similarity
Nucleotide bindingi286 – 2894ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:PFK1
Ordered Locus Names:YALI0D16357g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome D

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000112044Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains By similarity.

Protein-protein interaction databases

STRINGi4952.P59680.

Structurei

3D structure databases

ProteinModelPortaliP59680.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 558558N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni332 – 3343Substrate binding By similarity
Regioni376 – 3783Substrate binding By similarity
Regioni466 – 4694Substrate binding By similarity
Regioni559 – 57214Interdomain linkerUniRule annotationAdd
BLAST
Regioni573 – 953381C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
KOiK00850.
OMAiMIALNEN.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59680-1 [UniParc]FASTAAdd to Basket

« Hide

MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA    50
GSIQEVWLTS SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY 100
YAPDLDAVRA AITPFLHEDH STLLERPSHT NFIELYAIDP MGNLVGFSRR 150
ENPYSSAMQK PFSADDIGPQ NFSKPNETKI KGKKRIGVMT SGGDAPGMCA 200
AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED VRGWLSLGGT 250
LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW 300
PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL 350
DRICELVDFI DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE 400
AAPDATQWAE KMTRVVKRHR SQGKRKTVVI VAEGAIDSDL NPITAKMVKD 450
VLDGIGLDTR ISTLGHVQRG GPPVAADRVL ASLQGVEAID AILSLTPETP 500
SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR LRNPEFVEQL 550
QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR 600
GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC 650
DLGMIAYHFQ RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP 700
ATISNNVPGT DYSLGADTCL NSLVQYCDVL KTSASATRLR LFVVEVQGGN 750
SGYIATVAGL ITGAYVVYTP ESGINLRLLQ HDISYLKDTF AHQADVNRTG 800
KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV QQGGHPSPTD 850
RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK 900
TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII 950
DPC 953
Length:953
Mass (Da):103,753
Last modified:July 19, 2004 - v2
Checksum:i50DA57F8D774F74B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY142710 mRNA. Translation: AAN34943.2.
CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeqiXP_502897.1. XM_502897.1.

Genome annotation databases

EnsemblFungiiCAG81088; CAG81088; YALI0_D16357g.
GeneIDi2910306.
KEGGiyli:YALI0D16357g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY142710 mRNA. Translation: AAN34943.2 .
CR382130 Genomic DNA. Translation: CAG81088.1 .
RefSeqi XP_502897.1. XM_502897.1.

3D structure databases

ProteinModelPortali P59680.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4952.P59680.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAG81088 ; CAG81088 ; YALI0_D16357g .
GeneIDi 2910306.
KEGGi yli:YALI0D16357g.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
KOi K00850.
OMAi MIALNEN.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The phosphofructokinase from the yeast Yarrowia lipolytica."
    Flores C.L., Martinez-Costa O.H., Sanchez V., Aragon J.J., Gancedo C.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiPFKA_YARLI
AccessioniPrimary (citable) accession number: P59680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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