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P59680

- PFKA_YARLI

UniProt

P59680 - PFKA_YARLI

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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei193 – 1931ATP; via amide nitrogenUniRule annotation
Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
Active sitei334 – 3341Proton acceptorUniRule annotation
Binding sitei369 – 3691Substrate; shared with dimeric partnerUniRule annotation
Binding sitei433 – 4331SubstrateUniRule annotation
Binding sitei460 – 4601Substrate; shared with dimeric partnerUniRule annotation
Binding sitei645 – 6451Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei740 – 7401Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei807 – 8071Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei833 – 8331Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei906 – 9061Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2572ATPUniRule annotation
Nucleotide bindingi286 – 2894ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFK1
Ordered Locus Names:YALI0D16357g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome D

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953ATP-dependent 6-phosphofructokinasePRO_0000112044Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi4952.P59680.

Structurei

3D structure databases

ProteinModelPortaliP59680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 558558N-terminal catalytic PFK domain 1Add
BLAST
Regioni332 – 3343Substrate bindingUniRule annotation
Regioni376 – 3783Substrate bindingUniRule annotation
Regioni466 – 4694Substrate bindingUniRule annotation
Regioni559 – 57214Interdomain linkerAdd
BLAST
Regioni573 – 953381C-terminal regulatory PFK domain 2Add
BLAST
Regioni702 – 7065Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni747 – 7493Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni839 – 8424Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
InParanoidiP59680.
KOiK00850.
OMAiMIALNEN.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59680-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA
60 70 80 90 100
GSIQEVWLTS SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY
110 120 130 140 150
YAPDLDAVRA AITPFLHEDH STLLERPSHT NFIELYAIDP MGNLVGFSRR
160 170 180 190 200
ENPYSSAMQK PFSADDIGPQ NFSKPNETKI KGKKRIGVMT SGGDAPGMCA
210 220 230 240 250
AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED VRGWLSLGGT
260 270 280 290 300
LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW
310 320 330 340 350
PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL
360 370 380 390 400
DRICELVDFI DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE
410 420 430 440 450
AAPDATQWAE KMTRVVKRHR SQGKRKTVVI VAEGAIDSDL NPITAKMVKD
460 470 480 490 500
VLDGIGLDTR ISTLGHVQRG GPPVAADRVL ASLQGVEAID AILSLTPETP
510 520 530 540 550
SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR LRNPEFVEQL
560 570 580 590 600
QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR
610 620 630 640 650
GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC
660 670 680 690 700
DLGMIAYHFQ RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP
710 720 730 740 750
ATISNNVPGT DYSLGADTCL NSLVQYCDVL KTSASATRLR LFVVEVQGGN
760 770 780 790 800
SGYIATVAGL ITGAYVVYTP ESGINLRLLQ HDISYLKDTF AHQADVNRTG
810 820 830 840 850
KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV QQGGHPSPTD
860 870 880 890 900
RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK
910 920 930 940 950
TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII

DPC
Length:953
Mass (Da):103,753
Last modified:July 19, 2004 - v2
Checksum:i50DA57F8D774F74B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY142710 mRNA. Translation: AAN34943.2.
CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeqiXP_502897.1. XM_502897.1.

Genome annotation databases

EnsemblFungiiCAG81088; CAG81088; YALI0_D16357g.
GeneIDi2910306.
KEGGiyli:YALI0D16357g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY142710 mRNA. Translation: AAN34943.2 .
CR382130 Genomic DNA. Translation: CAG81088.1 .
RefSeqi XP_502897.1. XM_502897.1.

3D structure databases

ProteinModelPortali P59680.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4952.P59680.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAG81088 ; CAG81088 ; YALI0_D16357g .
GeneIDi 2910306.
KEGGi yli:YALI0D16357g.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
InParanoidi P59680.
KOi K00850.
OMAi MIALNEN.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The phosphofructokinase from the yeast Yarrowia lipolytica."
    Flores C.L., Martinez-Costa O.H., Sanchez V., Aragon J.J., Gancedo C.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiPFKA_YARLI
AccessioniPrimary (citable) accession number: P59680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 19, 2004
Last modified: November 26, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3