6-phosphofructokinase subunit alpha - Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
6-phosphofructokinase subunit alpha
EC=2.7.1.11

Alternative name(s):
6PF-1-K subunit alpha
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:	PFK1
Ordered Locus Names:	YALI0D16357g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia ›

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 953

953

6-phosphofructokinase subunit alpha

PRO_0000112044

Regions

Nucleotide binding

203 – 207

5

ATP By similarity

Nucleotide binding

361 – 365

5

ATP By similarity

Nucleotide binding

378 – 394

17

ATP By similarity

Sites

Active site

334

1

Proton acceptor By similarity

Metal binding

392

1

Magnesium; via carbonyl oxygen By similarity

Binding site

369

1

Substrate By similarity

Binding site

460

1

Substrate By similarity

Binding site

466

1

Substrate By similarity

Binding site

469

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P59680 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 50DA57F8D774F74B
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FASTA

953

103,753

        10         20         30         40         50         60 
MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA GSIQEVWLTS 

        70         80         90        100        110        120 
SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY YAPDLDAVRA AITPFLHEDH 

       130        140        150        160        170        180 
STLLERPSHT NFIELYAIDP MGNLVGFSRR ENPYSSAMQK PFSADDIGPQ NFSKPNETKI 

       190        200        210        220        230        240 
KGKKRIGVMT SGGDAPGMCA AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED 

       250        260        270        280        290        300 
VRGWLSLGGT LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW 

       310        320        330        340        350        360 
PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL DRICELVDFI 

       370        380        390        400        410        420 
DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE AAPDATQWAE KMTRVVKRHR 

       430        440        450        460        470        480 
SQGKRKTVVI VAEGAIDSDL NPITAKMVKD VLDGIGLDTR ISTLGHVQRG GPPVAADRVL 

       490        500        510        520        530        540 
ASLQGVEAID AILSLTPETP SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR 

       550        560        570        580        590        600 
LRNPEFVEQL QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR 

       610        620        630        640        650        660 
GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC DLGMIAYHFQ 

       670        680        690        700        710        720 
RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP ATISNNVPGT DYSLGADTCL 

       730        740        750        760        770        780 
NSLVQYCDVL KTSASATRLR LFVVEVQGGN SGYIATVAGL ITGAYVVYTP ESGINLRLLQ 

       790        800        810        820        830        840 
HDISYLKDTF AHQADVNRTG KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV 

       850        860        870        880        890        900 
QQGGHPSPTD RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK 

       910        920        930        940        950 
TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII DPC

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The phosphofructokinase from the yeast Yarrowia lipolytica." Flores C.L., Martinez-Costa O.H., Sanchez V., Aragon J.J., Gancedo C. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY142710 mRNA. Translation: AAN34943.2. CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeq	XP_502897.1. XM_502897.1.
3D structure databases
ProteinModelPortal	P59680.
ModBase	Search...
Protein-protein interaction databases
STRING	4952.P59680.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2910306.
KEGG	yli:YALI0D16357g.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000200154.
KO	K00850.
OrthoDB	EOG418FWH.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_YARLI

Accession

Primary (citable) accession number: P59680

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents