Reviewed, UniProtKB/Swiss-Prot P59680 (K6PF1_YARLI)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase subunit alpha
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase
    6PF-1-K subunit alpha

Gene names

Name:	PFK1
Ordered Locus Names:	YALI0D16357g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	6-phosphofructokinase subunit alpha		PRO_0000112044
Regions
	Nucleotide binding	203 – 207	5	ATP By similarity
	Nucleotide binding	361 – 365	5	ATP By similarity
	Nucleotide binding	378 – 394	17	ATP By similarity
Sites
	Active site	334	1	Proton acceptor By similarity
	Metal binding	392	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	369	1	Substrate By similarity
	Binding site	460	1	Substrate By similarity
	Binding site	466	1	Substrate By similarity
	Binding site	469	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59680-1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 50DA57F8D774F74B	FASTA	953	103,753
	10 20 30 40 50 60 MIEGISFASF VTHEKPKFVR ALDFYKALGF LPTKEYKHGT DHHATDEEGA GSIQEVWLTS 70 80 90 100 110 120 SRAGVPSVTV KLRLSRHGNE HVSLPNLKHD WRSLVPSLVY YAPDLDAVRA AITPFLHEDH 130 140 150 160 170 180 STLLERPSHT NFIELYAIDP MGNLVGFSRR ENPYSSAMQK PFSADDIGPQ NFSKPNETKI 190 200 210 220 230 240 KGKKRIGVMT SGGDAPGMCA AVRAVVRAGI ARGCEVYAVR EGYEGLVKGG DLIEPLSWED 250 260 270 280 290 300 VRGWLSLGGT LIGTARCKEF REREGRLAGA LNMVKNGIDA LIVIGGDGSL TGADLFREEW 310 320 330 340 350 360 PSLIEELVTN GSITAEQAER HRHLDICGMV GSIDNDMATT DVTIGAYSSL DRICELVDFI 370 380 390 400 410 420 DATAQSHSRA FVVEVMGRHC GWLALMAGTA TGADYIFIPE AAPDATQWAE KMTRVVKRHR 430 440 450 460 470 480 SQGKRKTVVI VAEGAIDSDL NPITAKMVKD VLDGIGLDTR ISTLGHVQRG GPPVAADRVL 490 500 510 520 530 540 ASLQGVEAID AILSLTPETP SPMIALNENK ITRKPLVESV ALTKKVADAI GNKDFAEAMR 550 560 570 580 590 600 LRNPEFVEQL QGFLLTNSAD KDRPQEPAKD PLRVAIVCTG APAGGMNAAI RSAVLYGLAR 610 620 630 640 650 660 GHQMFAIHNG WSGLVKNGDD AVRELTWLEV EPLCQKGGCE IGTNRSLPEC DLGMIAYHFQ 670 680 690 700 710 720 RQRFDGLIVI GGFEAFRALN QLDDARHAYP ALRIPMVGIP ATISNNVPGT DYSLGADTCL 730 740 750 760 770 780 NSLVQYCDVL KTSASATRLR LFVVEVQGGN SGYIATVAGL ITGAYVVYTP ESGINLRLLQ 790 800 810 820 830 840 HDISYLKDTF AHQADVNRTG KLLLRNERSS NVFTTDVITG IINEEAKGSF DARTAIPGHV 850 860 870 880 890 900 QQGGHPSPTD RVRAQRFAIK AVQFIEEHHG SKNNADHCVI LGVRGSKFKY TSVSHLYAHK 910 920 930 940 950 TEHGARRPKH SYWHAIGDIA NMLVGRKAPP LPETLNDEIE KNIAKEQGII DPC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The phosphofructokinase from the yeast Yarrowia lipolytica." Flores C.L., Martinez-Costa O.H., Sanchez V., Aragon J.J., Gancedo C. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY142710 mRNA. Translation: AAN34943.2. CR382130 Genomic DNA. Translation: CAG81088.1.
RefSeq	XP_502897.1.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ModBase	Search...
Protein-protein interaction databases
STRING	P59680.
Genome annotation databases
GeneID	2910306.
GenomeReviews	Gene locus YALI0D16357g in contig CR382130_GR.
KEGG	yli:YALI0D16357g.
Phylogenomic databases
HOGENOM	P59680.
Enzyme and pathway databases
BRENDA	2.7.1.11. 3602.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_YARLI

Accession

Primary (citable) accession number: P59680

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	July 19, 2004
Last modified:	September 22, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)