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Protein

Ankyrin repeat and SAM domain-containing protein 1A

Gene

Anks1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction.1 Publication

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB

GO - Biological processi

  • ephrin receptor signaling pathway Source: UniProtKB
  • negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
  • neuron remodeling Source: UniProtKB
  • regulation of ephrin receptor signaling pathway Source: MGI
  • substrate-dependent cell migration Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SAM domain-containing protein 1A
Alternative name(s):
Odin
Gene namesi
Name:Anks1a
Synonyms:Anks1, Kiaa0229, Odin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2446180. Anks1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell projection 1 Publication

  • Note: Cytoplasmic before and after growth factor treatment.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • neuron projection Source: UniProtKB
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 11501149Ankyrin repeat and SAM domain-containing protein 1APRO_0000066922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei335 – 3351PhosphothreonineBy similarity
Modified residuei524 – 5241PhosphoserineBy similarity
Modified residuei638 – 6381PhosphoserineBy similarity
Modified residuei640 – 6401PhosphoserineCombined sources
Modified residuei642 – 6421PhosphoserineCombined sources
Modified residuei644 – 6441PhosphoserineCombined sources
Modified residuei663 – 6631PhosphoserineCombined sources
Modified residuei677 – 6771PhosphoserineCombined sources
Modified residuei679 – 6791PhosphoserineCombined sources
Modified residuei682 – 6821PhosphoserineCombined sources
Modified residuei903 – 9031PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosine residues in response to EGF and PDGF.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP59672.
MaxQBiP59672.
PaxDbiP59672.
PRIDEiP59672.

PTM databases

iPTMnetiP59672.
PhosphoSiteiP59672.

Expressioni

Gene expression databases

BgeeiP59672.
CleanExiMM_ANKS1.
ExpressionAtlasiP59672. baseline and differential.
GenevisibleiP59672. MM.

Interactioni

Subunit structurei

Interacts (via SAM domain) with EPHA2 (via SAM domain) (By similarity). Interacts with EPHA8; EPHA8 kinase activity-independent but stimulated by EPHA8 ubiquitination.By similarity1 Publication

GO - Molecular functioni

  • ephrin receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiP59672. 1 interaction.
STRINGi10090.ENSMUSP00000110491.

Structurei

3D structure databases

ProteinModelPortaliP59672.
SMRiP59672. Positions 707-785, 949-1079.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati75 – 10430ANK 1Add
BLAST
Repeati108 – 13730ANK 2Add
BLAST
Repeati165 – 19430ANK 3Add
BLAST
Repeati198 – 22730ANK 4Add
BLAST
Repeati231 – 26030ANK 5Add
BLAST
Repeati263 – 29230ANK 6Add
BLAST
Domaini712 – 77867SAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini786 – 85368SAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini952 – 1107156PIDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 5121Gly-richAdd
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 2 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0507. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063104.
HOGENOMiHOG000033973.
HOVERGENiHBG050506.
InParanoidiP59672.
OrthoDBiEOG78H3SN.
TreeFamiTF320582.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
1.25.40.20. 4 hits.
2.30.29.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 3 hits.
PF00640. PID. 1 hit.
PF00536. SAM_1. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00462. PTB. 1 hit.
SM00454. SAM. 2 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 2 hits.
SSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS01179. PID. 1 hit.
PS50105. SAM_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P59672-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKEQELLEA ARTGHLPAVE KLLSGKRLSS GFGGGGGGSG SGGGSGGGGL
60 70 80 90 100
GSSSHPLSSL LSMWRGPNVN CVDSTGYTPL HHAALNGHRD VVEVLLRNDA
110 120 130 140 150
LTNVADSKGC YPLHLAAWKG DAQIVRLLIQ QGPSHTRVNE QNALEIRELK
160 170 180 190 200
KYGPFDPYIN AKNNDNETAL HCAAQYGHTE VVKALLEELT DPTMRNNKFE
210 220 230 240 250
TPLDLAALYG RLEVVKLLLG AHPNLLSCST RKHTPLHLAA RNGHKAVVQV
260 270 280 290 300
LLDAGMDSNY QTEMGSALHE AALFGKTDVV QILLAAGIDV NIKDNRGLTA
310 320 330 340 350
LDTVRDLPSQ KSQQIAALIE DHMTGKRSVK EVDRTSTAQL PLLSNTDAIA
360 370 380 390 400
PMSQGSMEKT VTELILHFDT HADEEGPYEA LYNAVSCHSL DSTASGRSSD
410 420 430 440 450
RDSMNKEAEA TGTRAAGVRP RERPPPPAKP PPDEEEEERV DKKYFPLAAS
460 470 480 490 500
EGLAVRPRIQ SSAPQEEEEH PYELLLTAET KKLGTTDGRT EDHRQSGSGR
510 520 530 540 550
SQDSVEGQDG QVPEQFSGLL HGSSPVCEVG QDPFQLLTAP SQSHPESSQQ
560 570 580 590 600
DACHEASMQL EEPGVQGTEP PQPGVPDQSK RVGLPAGLTA LASRTYLDAL
610 620 630 640 650
THTVPLRPAG AEEEDQSGPR SRAPPTSKPK AELKLSRSLS KSDSDLLTCS
660 670 680 690 700
PTEDATMGSR SESLSNCSIG KKRLEKSPSF ASEWDEIEKI MSSIGEGIDF
710 720 730 740 750
SQEQQKISGS RTLEQSVGEW LESIGLQQYE SKLLLNGFDD VRFLGSNVME
760 770 780 790 800
EQDLREIGIS DPQHRRKLLQ AARSLPKVKA LGYDGVSPTS VPSWLDSLGL
810 820 830 840 850
QDYVHSFLSS GYSSIDTVKN LWELELVNVL KVHLLGHRKR IIASLADRPY
860 870 880 890 900
EEPPQKPPRF SQLRCQDLIS QTSSPLSQND SCTGRSADLL LPSADTSRRR
910 920 930 940 950
HDSLPDPGTA SRADRFRVQE EPSETKLTLR PPSLAAPYAP VQSWQHQPEK
960 970 980 990 1000
LIFESCGYEA NYLGSMLIKD LRGTESTQDA CAKMRKSTEH MKKIPTIILS
1010 1020 1030 1040 1050
ITYKGVKFID ASNKNVIAEH EIRNISCAAQ DPEDLCTFAY ITKDLQTSHH
1060 1070 1080 1090 1100
YCHVFSTVDV NLTYEIILTL GQAFEVAYQL ALQAQKSRTM AASAASMIET
1110 1120 1130 1140 1150
KSSKPVPKPR VGMRKSALEP PDSDQEAPSH ASVSWIVDPK PDSKRSLSTN
Length:1,150
Mass (Da):125,242
Last modified:January 23, 2007 - v3
Checksum:i551AF44D1DB02D1B
GO
Isoform 2 (identifier: P59672-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-162: Missing.
     1117-1117: A → AVPVPPDSRCCHCHTCTTHRPSYLPLPSVSPGVK
     1150-1150: N → KYETTIF

Note: No experimental confirmation available.
Show »
Length:1,168
Mass (Da):127,104
Checksum:iECF09EDBC73A91C7
GO

Sequence cautioni

The sequence BAC97904.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei142 – 16221Missing in isoform 2. 1 PublicationVSP_012704Add
BLAST
Alternative sequencei1117 – 11171A → AVPVPPDSRCCHCHTCTTHR PSYLPLPSVSPGVK in isoform 2. 1 PublicationVSP_012705
Alternative sequencei1150 – 11501N → KYETTIF in isoform 2. 1 PublicationVSP_012706

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129094 mRNA. Translation: BAC97904.1. Different initiation.
BC050847 mRNA. Translation: AAH50847.1.
CCDSiCCDS37525.1. [P59672-1]
CCDS70778.1. [P59672-2]
RefSeqiNP_001272970.1. NM_001286041.1. [P59672-2]
NP_852078.1. NM_181413.4. [P59672-1]
UniGeneiMm.32556.

Genome annotation databases

EnsembliENSMUST00000088027; ENSMUSP00000085344; ENSMUSG00000024219. [P59672-2]
ENSMUST00000114842; ENSMUSP00000110491; ENSMUSG00000024219. [P59672-1]
GeneIDi224650.
KEGGimmu:224650.
UCSCiuc008bpw.2. mouse. [P59672-2]
uc008bpx.2. mouse. [P59672-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129094 mRNA. Translation: BAC97904.1. Different initiation.
BC050847 mRNA. Translation: AAH50847.1.
CCDSiCCDS37525.1. [P59672-1]
CCDS70778.1. [P59672-2]
RefSeqiNP_001272970.1. NM_001286041.1. [P59672-2]
NP_852078.1. NM_181413.4. [P59672-1]
UniGeneiMm.32556.

3D structure databases

ProteinModelPortaliP59672.
SMRiP59672. Positions 707-785, 949-1079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP59672. 1 interaction.
STRINGi10090.ENSMUSP00000110491.

PTM databases

iPTMnetiP59672.
PhosphoSiteiP59672.

Proteomic databases

EPDiP59672.
MaxQBiP59672.
PaxDbiP59672.
PRIDEiP59672.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088027; ENSMUSP00000085344; ENSMUSG00000024219. [P59672-2]
ENSMUST00000114842; ENSMUSP00000110491; ENSMUSG00000024219. [P59672-1]
GeneIDi224650.
KEGGimmu:224650.
UCSCiuc008bpw.2. mouse. [P59672-2]
uc008bpx.2. mouse. [P59672-1]

Organism-specific databases

CTDi224650.
MGIiMGI:2446180. Anks1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0507. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00530000063104.
HOGENOMiHOG000033973.
HOVERGENiHBG050506.
InParanoidiP59672.
OrthoDBiEOG78H3SN.
TreeFamiTF320582.

Miscellaneous databases

PROiP59672.
SOURCEiSearch...

Gene expression databases

BgeeiP59672.
CleanExiMM_ANKS1.
ExpressionAtlasiP59672. baseline and differential.
GenevisibleiP59672. MM.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
1.25.40.20. 4 hits.
2.30.29.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 3 hits.
PF00640. PID. 1 hit.
PF00536. SAM_1. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00462. PTB. 1 hit.
SM00454. SAM. 2 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 2 hits.
SSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS01179. PID. 1 hit.
PS50105. SAM_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic tail.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Limb.
  3. "Cloning of a novel phosphotyrosine binding domain containing molecule, Odin, involved in signaling by receptor tyrosine kinases."
    Pandey A., Blagoev B., Kratchmarova I., Fernandez M., Nielsen M., Kristiansen T.Z., Ohara O., Podtelejnikov A.V., Roche S., Lodish H.F., Mann M.
    Oncogene 21:8029-8036(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function."
    Shin J., Gu C., Park E., Park S.
    Mol. Cell. Biol. 27:8113-8126(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EFNA5-EPHA8 SIGNALING PATHWAY, INTERACTION WITH EPHA8, SUBCELLULAR LOCATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-640 AND SER-642, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642; SER-644; SER-663; SER-677; SER-679; SER-682 AND SER-903, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiANS1A_MOUSE
AccessioniPrimary (citable) accession number: P59672
Secondary accession number(s): Q6ZQG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.