P59672 (ANS1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat and SAM domain-containing protein 1A

Alternative name(s):
Odin

Gene names

Name:	Anks1a
Synonyms:	Anks1, Kiaa0229, Odin

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction. Ref.5
Subunit structure	Interacts with EPHA8; EPHA8 kinase activity-independent but stimulated by EPHA8 ubiquitination. Ref.5
Subcellular location	Cytoplasm By similarity. Cell projection. Note: Cytoplasmic before and after growth factor treatment By similarity. Ref.5
Post-translational modification	Phosphorylated on tyrosine residues in response to EGF and PDGF By similarity. Ref.3 Ref.4 Ref.6 Ref.7
Sequence similarities	Contains 6 ANK repeats. Contains 1 PID domain. Contains 2 SAM (sterile alpha motif) domains.
Sequence caution	The sequence BAC97904.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ephrin receptor signaling pathway Inferred from mutant phenotype Ref.5. Source: UniProtKB neuron remodeling Inferred from mutant phenotype Ref.5. Source: UniProtKB substrate-dependent cell migration Inferred from direct assay Ref.5. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell neuron projection Inferred from direct assay Ref.5. Source: UniProtKB
Molecular function	ephrin receptor binding Inferred from physical interaction Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P59672-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P59672-2)

The sequence of this isoform differs from the canonical sequence as follows:
     142-162: Missing.
     1117-1117: A → AVPVPPDSRCCHCHTCTTHRPSYLPLPSVSPGVK
     1150-1150: N → KYETTIF

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1150	1149	Ankyrin repeat and SAM domain-containing protein 1A		PRO_0000066922
Regions
	Repeat	75 – 104	30	ANK 1
	Repeat	108 – 137	30	ANK 2
	Repeat	165 – 194	30	ANK 3
	Repeat	198 – 227	30	ANK 4
	Repeat	231 – 260	30	ANK 5
	Repeat	263 – 292	30	ANK 6
	Domain	712 – 778	67	SAM 1
	Domain	786 – 853	68	SAM 2
	Domain	952 – 1107	156	PID
	Compositional bias	31 – 51	21	Gly-rich
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	335	1	Phosphothreonine By similarity
	Modified residue	472	1	Phosphotyrosine By similarity
	Modified residue	638	1	Phosphoserine By similarity
	Modified residue	640	1	Phosphoserine Ref.6
	Modified residue	642	1	Phosphoserine Ref.6
	Modified residue	644	1	Phosphoserine Ref.6
	Modified residue	663	1	Phosphoserine Ref.6
	Modified residue	677	1	Phosphoserine By similarity
	Modified residue	679	1	Phosphoserine Ref.4
	Modified residue	682	1	Phosphoserine Ref.7
	Modified residue	693	1	Phosphoserine By similarity
	Modified residue	903	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	142 – 162	21	Missing in isoform 2.		VSP_012704
	Alternative sequence	1117	1	A → AVPVPPDSRCCHCHTCTTHR PSYLPLPSVSPGVK in isoform 2.		VSP_012705
	Alternative sequence	1150	1	N → KYETTIF in isoform 2.		VSP_012706

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 551AF44D1DB02D1B	FASTA	1,150	125,242
	10 20 30 40 50 60 MGKEQELLEA ARTGHLPAVE KLLSGKRLSS GFGGGGGGSG SGGGSGGGGL GSSSHPLSSL 70 80 90 100 110 120 LSMWRGPNVN CVDSTGYTPL HHAALNGHRD VVEVLLRNDA LTNVADSKGC YPLHLAAWKG 130 140 150 160 170 180 DAQIVRLLIQ QGPSHTRVNE QNALEIRELK KYGPFDPYIN AKNNDNETAL HCAAQYGHTE 190 200 210 220 230 240 VVKALLEELT DPTMRNNKFE TPLDLAALYG RLEVVKLLLG AHPNLLSCST RKHTPLHLAA 250 260 270 280 290 300 RNGHKAVVQV LLDAGMDSNY QTEMGSALHE AALFGKTDVV QILLAAGIDV NIKDNRGLTA 310 320 330 340 350 360 LDTVRDLPSQ KSQQIAALIE DHMTGKRSVK EVDRTSTAQL PLLSNTDAIA PMSQGSMEKT 370 380 390 400 410 420 VTELILHFDT HADEEGPYEA LYNAVSCHSL DSTASGRSSD RDSMNKEAEA TGTRAAGVRP 430 440 450 460 470 480 RERPPPPAKP PPDEEEEERV DKKYFPLAAS EGLAVRPRIQ SSAPQEEEEH PYELLLTAET 490 500 510 520 530 540 KKLGTTDGRT EDHRQSGSGR SQDSVEGQDG QVPEQFSGLL HGSSPVCEVG QDPFQLLTAP 550 560 570 580 590 600 SQSHPESSQQ DACHEASMQL EEPGVQGTEP PQPGVPDQSK RVGLPAGLTA LASRTYLDAL 610 620 630 640 650 660 THTVPLRPAG AEEEDQSGPR SRAPPTSKPK AELKLSRSLS KSDSDLLTCS PTEDATMGSR 670 680 690 700 710 720 SESLSNCSIG KKRLEKSPSF ASEWDEIEKI MSSIGEGIDF SQEQQKISGS RTLEQSVGEW 730 740 750 760 770 780 LESIGLQQYE SKLLLNGFDD VRFLGSNVME EQDLREIGIS DPQHRRKLLQ AARSLPKVKA 790 800 810 820 830 840 LGYDGVSPTS VPSWLDSLGL QDYVHSFLSS GYSSIDTVKN LWELELVNVL KVHLLGHRKR 850 860 870 880 890 900 IIASLADRPY EEPPQKPPRF SQLRCQDLIS QTSSPLSQND SCTGRSADLL LPSADTSRRR 910 920 930 940 950 960 HDSLPDPGTA SRADRFRVQE EPSETKLTLR PPSLAAPYAP VQSWQHQPEK LIFESCGYEA 970 980 990 1000 1010 1020 NYLGSMLIKD LRGTESTQDA CAKMRKSTEH MKKIPTIILS ITYKGVKFID ASNKNVIAEH 1030 1040 1050 1060 1070 1080 EIRNISCAAQ DPEDLCTFAY ITKDLQTSHH YCHVFSTVDV NLTYEIILTL GQAFEVAYQL 1090 1100 1110 1120 1130 1140 ALQAQKSRTM AASAASMIET KSSKPVPKPR VGMRKSALEP PDSDQEAPSH ASVSWIVDPK 1150 PDSKRSLSTN
	Isoform 2 [UniParc]. Checksum: ECF09EDBC73A91C7	FASTA	1,168	127,104
	10 20 30 40 50 60 MGKEQELLEA ARTGHLPAVE KLLSGKRLSS GFGGGGGGSG SGGGSGGGGL GSSSHPLSSL 70 80 90 100 110 120 LSMWRGPNVN CVDSTGYTPL HHAALNGHRD VVEVLLRNDA LTNVADSKGC YPLHLAAWKG 130 140 150 160 170 180 DAQIVRLLIQ QGPSHTRVNE QNNDNETALH CAAQYGHTEV VKALLEELTD PTMRNNKFET 190 200 210 220 230 240 PLDLAALYGR LEVVKLLLGA HPNLLSCSTR KHTPLHLAAR NGHKAVVQVL LDAGMDSNYQ 250 260 270 280 290 300 TEMGSALHEA ALFGKTDVVQ ILLAAGIDVN IKDNRGLTAL DTVRDLPSQK SQQIAALIED 310 320 330 340 350 360 HMTGKRSVKE VDRTSTAQLP LLSNTDAIAP MSQGSMEKTV TELILHFDTH ADEEGPYEAL 370 380 390 400 410 420 YNAVSCHSLD STASGRSSDR DSMNKEAEAT GTRAAGVRPR ERPPPPAKPP PDEEEEERVD 430 440 450 460 470 480 KKYFPLAASE GLAVRPRIQS SAPQEEEEHP YELLLTAETK KLGTTDGRTE DHRQSGSGRS 490 500 510 520 530 540 QDSVEGQDGQ VPEQFSGLLH GSSPVCEVGQ DPFQLLTAPS QSHPESSQQD ACHEASMQLE 550 560 570 580 590 600 EPGVQGTEPP QPGVPDQSKR VGLPAGLTAL ASRTYLDALT HTVPLRPAGA EEEDQSGPRS 610 620 630 640 650 660 RAPPTSKPKA ELKLSRSLSK SDSDLLTCSP TEDATMGSRS ESLSNCSIGK KRLEKSPSFA 670 680 690 700 710 720 SEWDEIEKIM SSIGEGIDFS QEQQKISGSR TLEQSVGEWL ESIGLQQYES KLLLNGFDDV 730 740 750 760 770 780 RFLGSNVMEE QDLREIGISD PQHRRKLLQA ARSLPKVKAL GYDGVSPTSV PSWLDSLGLQ 790 800 810 820 830 840 DYVHSFLSSG YSSIDTVKNL WELELVNVLK VHLLGHRKRI IASLADRPYE EPPQKPPRFS 850 860 870 880 890 900 QLRCQDLISQ TSSPLSQNDS CTGRSADLLL PSADTSRRRH DSLPDPGTAS RADRFRVQEE 910 920 930 940 950 960 PSETKLTLRP PSLAAPYAPV QSWQHQPEKL IFESCGYEAN YLGSMLIKDL RGTESTQDAC 970 980 990 1000 1010 1020 AKMRKSTEHM KKIPTIILSI TYKGVKFIDA SNKNVIAEHE IRNISCAAQD PEDLCTFAYI 1030 1040 1050 1060 1070 1080 TKDLQTSHHY CHVFSTVDVN LTYEIILTLG QAFEVAYQLA LQAQKSRTMA ASAASMIETK 1090 1100 1110 1120 1130 1140 SSKPVPKPRV GMRKSAVPVP PDSRCCHCHT CTTHRPSYLP LPSVSPGVKL EPPDSDQEAP 1150 1160 SHASVSWIVD PKPDSKRSLS TKYETTIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Embryonic tail.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Limb.
[3]	"Cloning of a novel phosphotyrosine binding domain containing molecule, Odin, involved in signaling by receptor tyrosine kinases." Pandey A., Blagoev B., Kratchmarova I., Fernandez M., Nielsen M., Kristiansen T.Z., Ohara O., Podtelejnikov A.V., Roche S., Lodish H.F., Mann M. Oncogene 21:8029-8036(2002) [PubMed: 12439753] [Abstract] Cited for: PHOSPHORYLATION.
[4]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-679, MASS SPECTROMETRY. Tissue: Embryonic brain.
[5]	"Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function." Shin J., Gu C., Park E., Park S. Mol. Cell. Biol. 27:8113-8126(2007) [PubMed: 17875921] [Abstract] Cited for: FUNCTION IN EFNA5-EPHA8 SIGNALING PATHWAY, INTERACTION WITH EPHA8, SUBCELLULAR LOCATION.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-640; SER-642; SER-644 AND SER-663, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-682 AND SER-903, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK129094 mRNA. Translation: BAC97904.1. Different initiation. BC050847 mRNA. Translation: AAH50847.1.
IPI	IPI00346041. IPI00754201.
RefSeq	NP_852078.1. NM_181413.3.
UniGene	Mm.32556.
3D structure databases
ProteinModelPortal	P59672.
SMR	P59672. Positions 4-322, 715-848, 949-1085.
ModBase	Search...
Protein-protein interaction databases
IntAct	P59672. 1 interaction.
STRING	P59672.
PTM databases
PhosphoSite	P59672.
Proteomic databases
PRIDE	P59672.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000088027; ENSMUSP00000085344; ENSMUSG00000024219. ENSMUST00000114842; ENSMUSP00000110491; ENSMUSG00000024219.
GeneID	224650.
KEGG	mmu:224650.
Organism-specific databases
CTD	224650.
MGI	MGI:2446180. Anks1.
Rouge	Search...
Phylogenomic databases
GeneTree	ENSGT00530000063104.
HOVERGEN	HBG050506.
OrthoDB	EOG4PVNXV.
Gene expression databases
ArrayExpress	P59672.
Bgee	P59672.
CleanEx	MM_ANKS1.
Genevestigator	P59672.
GermOnline	ENSMUSG00000024219. Mus musculus.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR011993. PH_type. IPR006020. PTyr_interaction_dom. IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 2 hits. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 2 hits.
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 3 hits. PF00640. PID. 1 hit. PF00536. SAM_1. 1 hit. PF07647. SAM_2. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 6 hits. SM00462. PTB. 1 hit. SM00454. SAM. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF47769. SAM_homology. 2 hits.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 5 hits. PS01179. PID. 1 hit. PS50105. SAM_DOMAIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	377283.
SOURCE	Search...

Entry information

Entry name

ANS1A_MOUSE

Accession

Primary (citable) accession number: P59672
Secondary accession number(s): Q6ZQG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program