P59668 (FAD12_MORIS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Delta(12) fatty acid desaturase EC=1.14.19.6 Alternative name(s): Delta-12 fatty acid desaturase
Organism	Mortierella isabellina (Umbelopsis isabellina)
Taxonomic identifier	91625 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Fungi incertae sedis › Early diverging fungal lineages › Mucoromycotina › Mucorales › Mucorales incertae sedis › Umbelopsis

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the desaturation of oleic acid (Delta(9)-18:1) to linoleic acid (Delta9, Delta(12)-18:2) By similarity.
Catalytic activity	Acyl-CoA + reduced acceptor + O2 = Delta(12)-acyl-CoA + acceptor + 2 H2O.
Pathway	Lipid metabolism; polyunsaturated fatty acid biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	unsaturated fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Delta(12) fatty acid desaturase		PRO_0000185423
Regions
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Transmembrane	277 – 297	21	Helical; Potential
	Motif	112 – 116	5	Histidine box-1
	Motif	148 – 152	5	Histidine box-2
	Motif	339 – 343	5	Histidine box-3

Sequences

	Sequence		Length	Mass (Da)
	P59668 [UniParc]. Last modified April 30, 2003. Version 1. Checksum: F5512D3F8210DBD2	FASTA	400	46,016
	10 20 30 40 50 60 MAPPNTIDAG LTQRHITTTA APTSAKPAFE RNYQLPEFTI KEIRECIPAH CFERSGLRGL 70 80 90 100 110 120 CHVAIDLTWA SLLFLAATQI DKFENPLIRY LAWPAYWIMQ GIVCTGIWVL AHECGHQSFS 130 140 150 160 170 180 TSKTLNNTVG WILHSMLLVP YHSWRISHSK HHKATGHMTK DQVFVPKTRS QVGLPPKESA 190 200 210 220 230 240 AAAVQEEDMS VHLDEEAPIV TLFWMVIQFL FGWPAYLIMN ASGQDYGRWT SHFHTYSPIF 250 260 270 280 290 300 EPRNFFDIII SDLGVLAALG ALIYASMQLS LLTVTKYYII PYLFVNFWLV LITFLQHTDP 310 320 330 340 350 360 KLPHYREGAW NFQRGALCTV DRSFGKFLDH MFHGIVHTHV AHHLFSQMPF YHAEEATYHL 370 380 390 400 KKLLGEYYVY DPSPIVVAVW RSFRECRFVE DHGDVVFFKK

References

[1]	"Delta 12 fatty acid desturase mRNA of Mortierella isabellina." Liu L., Li M., Xing L., Hu G. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: M6-22.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF417245 mRNA. Translation: AAL13301.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00658.
Family and domain databases
InterPro	IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Pfam	PF00487. FA_desaturase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD12_MORIS

Accession

Primary (citable) accession number: P59668

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	October 3, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program