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P59666

- DEF3_HUMAN

UniProt

P59666 - DEF3_HUMAN

Protein

Neutrophil defensin 3

Gene

DEFA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.4 Publications

    GO - Biological processi

    1. antibacterial humoral response Source: UniProt
    2. defense response to fungus Source: UniProtKB-KW
    3. defense response to Gram-positive bacterium Source: UniProt
    4. defense response to virus Source: UniProtKB-KW
    5. innate immune response Source: UniProt
    6. innate immune response in mucosa Source: UniProt
    7. intracellular estrogen receptor signaling pathway Source: UniProt
    8. killing of cells of other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Defensin, Fungicide

    Keywords - Biological processi

    Antiviral defense

    Enzyme and pathway databases

    ReactomeiREACT_115574. Alpha-defensins.
    REACT_115846. Defensins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutrophil defensin 3
    Alternative name(s):
    Defensin, alpha 3
    HNP-3
    Short name:
    HP-3
    Short name:
    HP3
    Cleaved into the following 2 chains:
    Alternative name(s):
    HNP-2
    Short name:
    HP-2
    Short name:
    HP2
    Gene namesi
    Name:DEFA3
    Synonyms:DEF3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:2762. DEFA3.

    Subcellular locationi

    GO - Cellular componenti

    1. azurophil granule lumen Source: Reactome
    2. extracellular region Source: Reactome
    3. extracellular space Source: UniProt
    4. Golgi lumen Source: Reactome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27239.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Propeptidei20 – 3819PRO_0000006777Add
    BLAST
    Chaini39 – 9456HP 3-56PRO_0000006778Add
    BLAST
    Peptidei65 – 9430Neutrophil defensin 3PRO_0000006779Add
    BLAST
    Peptidei66 – 9429Neutrophil defensin 2PRO_0000006780Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi66 ↔ 94
    Disulfide bondi68 ↔ 83
    Disulfide bondi73 ↔ 93

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP59666.
    PRIDEiP59666.

    PTM databases

    PhosphoSiteiP59666.

    Miscellaneous databases

    PMAP-CutDBP59666.

    Expressioni

    Gene expression databases

    BgeeiP59666.
    CleanExiHS_DEFA3.
    GenevestigatoriP59666.

    Organism-specific databases

    HPAiHPA052517.

    Interactioni

    Subunit structurei

    Dimer.2 Publications

    Protein-protein interaction databases

    IntActiP59666. 2 interactions.
    MINTiMINT-1409240.
    STRINGi9606.ENSP00000328359.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi67 – 715
    Beta strandi78 – 858
    Beta strandi88 – 947

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DFNX-ray1.90A/B65-94[»]
    1ZMHX-ray1.50A/B/C/D66-94[»]
    1ZMIX-ray1.15A/B/C/D66-94[»]
    1ZMKX-ray1.30A/B66-94[»]
    2PM4X-ray1.95A/B65-94[»]
    2PM5X-ray2.40A/B65-94[»]
    ProteinModelPortaliP59666.
    SMRiP59666. Positions 66-94.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP59666.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alpha-defensin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG86943.
    HOGENOMiHOG000233351.
    HOVERGENiHBG011703.
    InParanoidiP59666.
    KOiK05230.
    OMAiQERDDEA.
    OrthoDBiEOG75TMFQ.
    PhylomeDBiP59666.
    TreeFamiTF338414.

    Family and domain databases

    InterProiIPR016327. Alpha-defensin.
    IPR006080. Defensin_beta/neutrophil.
    IPR002366. Defensin_propep.
    IPR006081. Mammalian_defensins.
    [Graphical view]
    PANTHERiPTHR11876. PTHR11876. 1 hit.
    PfamiPF00323. Defensin_1. 1 hit.
    PF00879. Defensin_propep. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
    SMARTiSM00048. DEFSN. 1 hit.
    [Graphical view]
    PROSITEiPS00269. DEFENSIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P59666-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTLAILAAI LLVALQAQAE PLQARADEVA AAPEQIAADI PEVVVSLAWD   50
    ESLAPKHPGS RKNMDCYCRI PACIAGERRY GTCIYQGRLW AFCC 94
    Length:94
    Mass (Da):10,245
    Last modified:April 30, 2003 - v1
    Checksum:i0E0F8E957376C6AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21131 mRNA. Translation: AAA35753.2.
    M23281 mRNA. Translation: AAA52304.1.
    L12691 Genomic DNA. Translation: AAB57722.1.
    X13621 mRNA. Translation: CAA31952.1.
    BC027917 mRNA. Translation: AAH27917.1.
    CCDSiCCDS5962.1.
    PIRiC40499.
    RefSeqiNP_005208.1. NM_005217.3.
    UniGeneiHs.654448.

    Genome annotation databases

    EnsembliENST00000327857; ENSP00000328359; ENSG00000239839.
    GeneIDi1668.
    KEGGihsa:1668.

    Polymorphism databases

    DMDMi30316323.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21131 mRNA. Translation: AAA35753.2 .
    M23281 mRNA. Translation: AAA52304.1 .
    L12691 Genomic DNA. Translation: AAB57722.1 .
    X13621 mRNA. Translation: CAA31952.1 .
    BC027917 mRNA. Translation: AAH27917.1 .
    CCDSi CCDS5962.1.
    PIRi C40499.
    RefSeqi NP_005208.1. NM_005217.3.
    UniGenei Hs.654448.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DFN X-ray 1.90 A/B 65-94 [» ]
    1ZMH X-ray 1.50 A/B/C/D 66-94 [» ]
    1ZMI X-ray 1.15 A/B/C/D 66-94 [» ]
    1ZMK X-ray 1.30 A/B 66-94 [» ]
    2PM4 X-ray 1.95 A/B 65-94 [» ]
    2PM5 X-ray 2.40 A/B 65-94 [» ]
    ProteinModelPortali P59666.
    SMRi P59666. Positions 66-94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P59666. 2 interactions.
    MINTi MINT-1409240.
    STRINGi 9606.ENSP00000328359.

    PTM databases

    PhosphoSitei P59666.

    Polymorphism databases

    DMDMi 30316323.

    Proteomic databases

    PaxDbi P59666.
    PRIDEi P59666.

    Protocols and materials databases

    DNASUi 1668.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000327857 ; ENSP00000328359 ; ENSG00000239839 .
    GeneIDi 1668.
    KEGGi hsa:1668.

    Organism-specific databases

    CTDi 1668.
    GeneCardsi GC08M006875.
    HGNCi HGNC:2762. DEFA3.
    HPAi HPA052517.
    MIMi 604522. gene.
    neXtProti NX_P59666.
    PharmGKBi PA27239.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG86943.
    HOGENOMi HOG000233351.
    HOVERGENi HBG011703.
    InParanoidi P59666.
    KOi K05230.
    OMAi QERDDEA.
    OrthoDBi EOG75TMFQ.
    PhylomeDBi P59666.
    TreeFami TF338414.

    Enzyme and pathway databases

    Reactomei REACT_115574. Alpha-defensins.
    REACT_115846. Defensins.

    Miscellaneous databases

    EvolutionaryTracei P59666.
    GeneWikii DEFA3.
    GenomeRNAii 1668.
    NextBioi 6864.
    PMAP-CutDB P59666.
    PROi P59666.
    SOURCEi Search...

    Gene expression databases

    Bgeei P59666.
    CleanExi HS_DEFA3.
    Genevestigatori P59666.

    Family and domain databases

    InterProi IPR016327. Alpha-defensin.
    IPR006080. Defensin_beta/neutrophil.
    IPR002366. Defensin_propep.
    IPR006081. Mammalian_defensins.
    [Graphical view ]
    PANTHERi PTHR11876. PTHR11876. 1 hit.
    Pfami PF00323. Defensin_1. 1 hit.
    PF00879. Defensin_propep. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001875. Alpha-defensin. 1 hit.
    SMARTi SM00048. DEFSN. 1 hit.
    [Graphical view ]
    PROSITEi PS00269. DEFENSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of human defensin cDNA clones."
      Daher K.A., Lehrer R.I., Ganz T., Kronenberg M.
      Proc. Natl. Acad. Sci. U.S.A. 85:7327-7331(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Differentiation stage-specific expression of a gene during granulopoiesis."
      Wiedemann L.M., Francis G.E., Lamb R.F., Burns J.H., Winnie J.N., McKenzie E.D., Birnie G.D.
      Leukemia 3:227-234(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The structure of neutrophil defensin genes."
      Linzmeier R., Michaelson D., Liu L., Ganz T.
      FEBS Lett. 321:267-273(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas and Spleen.
    5. Cited for: PROTEIN SEQUENCE OF 65-94.
    6. "Determination of the disulfide array in the human defensin HNP-2. A covalently cyclized peptide."
      Selsted M.E., Harwig S.S.L.
      J. Biol. Chem. 264:4003-4007(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.
    7. "Posttranslational processing of defensins in immature human myeloid cells."
      Valore E.V., Ganz T.
      Blood 79:1538-1544(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING.
    8. "Antibacterial activity and specificity of the six human alpha-defensins."
      Ericksen B., Wu Z., Lu W., Lehrer R.I.
      Antimicrob. Agents Chemother. 49:269-275(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization."
      Hill C.P., Yee J., Selsted M.E., Eisenberg D.
      Science 251:1481-1485(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF DEFENSIN 3, SUBUNIT, FUNCTION.
    10. "Reconstruction of the conserved beta-bulge in mammalian defensins using D-amino acids."
      Xie C., Prahl A., Ericksen B., Wu Z., Zeng P., Li X., Lu W.-Y., Lubkowski J., Lu W.
      J. Biol. Chem. 280:32921-32929(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS) OF 66-94, DISULFIDE BONDS, FUNCTION.
    11. "Toward understanding the cationicity of defensins. Arg and Lys versus their noncoded analogs."
      Zou G., de Leeuw E., Li C., Pazgier M., Li C., Zeng P., Lu W.-Y., Lubkowski J., Lu W.
      J. Biol. Chem. 282:19653-19665(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 65-94, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiDEF3_HUMAN
    AccessioniPrimary (citable) accession number: P59666
    Secondary accession number(s): P11479, Q14125
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3