Reviewed,
UniProtKB/Swiss-Prot P59666 (DEF3_HUMAN)
Last modified
June 16, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Neutrophil defensin 3 Alternative name(s): HNP-3 Short name=HP-3 Short name=HP3 Defensin, alpha 3 Cleaved into the following 2 chains: 1- Recommended name: HP 3-56 2- Recommended name: Neutrophil defensin 2 Alternative name(s): HNP-2 Short name=HP-2 Short name=HP2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 94 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Ref.9 Ref.11 Ref.12 Ref.13 |
| Subunit structure | |
| Subcellular location | |
| Sequence similarities | Belongs to the alpha-defensin family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antiviral defense |
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Antibiotic Antimicrobial Defensin Fungicide |
| PTM | Disulfide bond Phosphoprotein |
| Technical term | 3D-structure Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungusInferred from electronic annotation. Source: UniProtKB-KW response to virusInferred from electronic annotation. Source: UniProtKB-KW xenobiotic metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular space Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||
Molecule processing | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | ||||||||||||
| Propeptide | 20 – 38 | 19 | PRO_0000006777 | |||||||||||
| Chain | 39 – 94 | 56 | HP 3-56 | PRO_0000006778 | ||||||||||
| Peptide | 65 – 94 | 30 | Neutrophil defensin 3 | PRO_0000006779 | ||||||||||
| Peptide | 66 – 94 | 29 | Neutrophil defensin 2 | PRO_0000006780 | ||||||||||
Amino acid modifications | ||||||||||||||
| Modified residue | 85 | 1 | Phosphotyrosine Ref.10 | |||||||||||
| Disulfide bond | 66 ↔ 94 | Ref.12 Ref.7 | ||||||||||||
| Disulfide bond | 68 ↔ 83 | Ref.12 Ref.7 | ||||||||||||
| Disulfide bond | 73 ↔ 93 | Ref.12 Ref.7 | ||||||||||||
Secondary structure | ||||||||||||||
Helix Strand Turn | ||||||||||||||
| Beta strand | 67 – 71 | 5 | ||||||||||||
| Beta strand | 78 – 85 | 8 | ||||||||||||
| Beta strand | 88 – 94 | 7 | ||||||||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of human defensin cDNA clones." Daher K.A., Lehrer R.I., Ganz T., Kronenberg M. Proc. Natl. Acad. Sci. U.S.A. 85:7327-7331(1988) [PubMed: 3174637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Differentiation stage-specific expression of a gene during granulopoiesis." Wiedemann L.M., Francis G.E., Lamb R.F., Burns J.H., Winnie J.N., McKenzie E.D., Birnie G.D. Leukemia 3:227-234(1989) [PubMed: 2918759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The structure of neutrophil defensin genes." Linzmeier R., Michaelson D., Liu L., Ganz T. FEBS Lett. 321:267-273(1993) [PubMed: 8477861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | Erratum Linzmeier R., Michaelson D., Liu L., Ganz T. FEBS Lett. 326:299-300(1993) [PubMed: 8325384] [Abstract] |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen. |
| [6] | "Primary structures of three human neutrophil defensins." Selsted M.E., Harwig S.S.L., Ganz T., Schilling J.W., Lehrer R.I. J. Clin. Invest. 76:1436-1439(1985) [PubMed: 4056036] [Abstract] Cited for: PROTEIN SEQUENCE OF 65-94. |
| [7] | "Determination of the disulfide array in the human defensin HNP-2. A covalently cyclized peptide." Selsted M.E., Harwig S.S.L. J. Biol. Chem. 264:4003-4007(1989) [PubMed: 2917986] [Abstract] Cited for: DISULFIDE BONDS. |
| [8] | "Posttranslational processing of defensins in immature human myeloid cells." Valore E.V., Ganz T. Blood 79:1538-1544(1992) [PubMed: 1339298] [Abstract] Cited for: PROTEOLYTIC PROCESSING. |
| [9] | "Antibacterial activity and specificity of the six human alpha-defensins." Ericksen B., Wu Z., Lu W., Lehrer R.I. Antimicrob. Agents Chemother. 49:269-275(2005) [PubMed: 15616305] [Abstract] Cited for: FUNCTION. |
| [10] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-85, MASS SPECTROMETRY. |
| [11] | "Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization." Hill C.P., Yee J., Selsted M.E., Eisenberg D. Science 251:1481-1485(1991) [PubMed: 2006422] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF DEFENSIN 3, SUBUNIT, FUNCTION. |
| [12] | "Reconstruction of the conserved beta-bulge in mammalian defensins using D-amino acids." Xie C., Prahl A., Ericksen B., Wu Z., Zeng P., Li X., Lu W.-Y., Lubkowski J., Lu W. J. Biol. Chem. 280:32921-32929(2005) [PubMed: 15894545] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS) OF 66-94, DISULFIDE BONDS, FUNCTION. |
| [13] | "Toward understanding the cationicity of defensins. Arg and Lys versus their noncoded analogs." Zou G., de Leeuw E., Li C., Pazgier M., Li C., Zeng P., Lu W.-Y., Lubkowski J., Lu W. J. Biol. Chem. 282:19653-19665(2007) [PubMed: 17452329] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 65-94, FUNCTION, SUBUNIT. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M21131 mRNA. Translation: AAA35753.2. M23281 mRNA. Translation: AAA52304.1. L12691 Genomic DNA. Translation: AAB57722.1. X13621 mRNA. Translation: CAA31952.1. BC027917 mRNA. Translation: AAH27917.1. | |||||||||||||||||||||||||||||||||||||||||||
| IPI | IPI00021827. | ||||||||||||||||||||||||||||||||||||||||||
| PIR | C40499. | ||||||||||||||||||||||||||||||||||||||||||
| RefSeq | NP_005208.1. | ||||||||||||||||||||||||||||||||||||||||||
| UniGene | Hs.654448 | ||||||||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||||||||||||||
| PhosphoSite | P59666. | ||||||||||||||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||||||||||||||
| PRIDE | P59666. | ||||||||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||||||||
| Ensembl | ENSG00000185918. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||||||||||||||
| GeneID | 1668. | ||||||||||||||||||||||||||||||||||||||||||
| KEGG | hsa:1668. | ||||||||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||||||||
| GeneCards | GC08M006860. | ||||||||||||||||||||||||||||||||||||||||||
| H-InvDB | HIX0007285. | ||||||||||||||||||||||||||||||||||||||||||
| HGNC | HGNC:2762. DEFA3. | ||||||||||||||||||||||||||||||||||||||||||
| MIM | 604522. gene. | ||||||||||||||||||||||||||||||||||||||||||
| PharmGKB | PA27239. | ||||||||||||||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||||||||
| HOGENOM | P59666. | ||||||||||||||||||||||||||||||||||||||||||
| HOVERGEN | P59666. | ||||||||||||||||||||||||||||||||||||||||||
| OMA | P59666. PRCTATE. | ||||||||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||||||||
| CleanEx | HS_DEFA3. | ||||||||||||||||||||||||||||||||||||||||||
| GermOnline | ENSG00000185918. Homo sapiens. | ||||||||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||||||||
| InterPro | IPR016327. Alpha-defensin. IPR006081. Defensin_alpha. IPR006080. Defensin_mammal. IPR002366. Defensin_propep. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| Pfam | PF00323. Defensin_1. 1 hit. PF00879. Defensin_propep. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PIRSF | PIRSF001875. Alpha-defensin. 1 hit. | ||||||||||||||||||||||||||||||||||||||||||
| SMART | SM00048. DEFSN. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PROSITE | PS00269. DEFENSIN. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||||||||
| NextBio | 6864. | ||||||||||||||||||||||||||||||||||||||||||
| PMAP-CutDB | P59666. | ||||||||||||||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | DEF3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P59666 Secondary accession number(s): P11479, Q14125 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 8 Human chromosome 8: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


