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Protein

Neutrophil defensin 1

Gene

DEFA1

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.2 Publications

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
  • antimicrobial humoral response Source: Reactome
  • chemotaxis Source: ProtInc
  • defense response to fungus Source: UniProtKB-KW
  • defense response to Gram-positive bacterium Source: UniProtKB
  • defense response to protozoan Source: UniProtKB
  • defense response to virus Source: UniProtKB-KW
  • disruption by host of symbiont cells Source: UniProtKB
  • immune response Source: ProtInc
  • innate immune response in mucosa Source: UniProtKB
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • pore formation in membrane of other organism during symbiotic interaction Source: UniProtKB
  • T cell chemotaxis Source: UniProtKB

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Defensin, Fungicide
Biological processAntiviral defense

Enzyme and pathway databases

ReactomeiR-HSA-1461973 Defensins
R-HSA-1462054 Alpha-defensins
R-HSA-6798695 Neutrophil degranulation

Protein family/group databases

TCDBi1.C.19.1.1 the defensin (defensin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil defensin 1
Alternative name(s):
Defensin, alpha 1
HNP-1
Short name:
HP-1
Short name:
HP1
Cleaved into the following 2 chains:
Alternative name(s):
HNP-2
Short name:
HP-2
Short name:
HP2
Gene namesi
Name:DEFA1
Synonyms:DEF1, DEFA2, MRS
AND
Name:DEFA1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000206047.2
HostDB:ENSG00000240247.6
HGNCiHGNC:2761 DEFA1
HGNC:33596 DEFA1B
MIMi125220 gene
neXtProtiNX_P59665

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1667
728358
OpenTargetsiENSG00000206047
ENSG00000240247
PharmGKBiPA165585475

Polymorphism and mutation databases

DMDMi30316322

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
PropeptideiPRO_000000677120 – 38Add BLAST19
ChainiPRO_000000677239 – 94HP 1-56Add BLAST56
PeptideiPRO_000000677365 – 94Neutrophil defensin 1Add BLAST30
PeptideiPRO_000000677466 – 94Neutrophil defensin 2Add BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 941 Publication
Disulfide bondi68 ↔ 831 Publication
Disulfide bondi73 ↔ 931 Publication
Modified residuei78ADP-ribosylarginine; by ART11 Publication1
Modified residuei85Phosphotyrosine1 Publication1
Modified residuei88ADP-ribosylarginine; by ART11 Publication1

Post-translational modificationi

ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production.
Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation.1 Publication

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP59665
PeptideAtlasiP59665
PRIDEiP59665
TopDownProteomicsiP59665

PTM databases

iPTMnetiP59665
PhosphoSitePlusiP59665

Miscellaneous databases

PMAP-CutDBiP59665

Expressioni

Gene expression databases

BgeeiENSG00000206047
CleanExiHS_DEFA1
GenevisibleiP59665 HS

Organism-specific databases

HPAiCAB032548
HPA052517

Interactioni

Subunit structurei

Dimer (PubMed:17452329). Interacts with RETN (PubMed:15064728).2 Publications

Protein-protein interaction databases

BioGridi108031, 93 interactors
608786, 4 interactors
IntActiP59665, 6 interactors
MINTiP59665
STRINGi9606.ENSP00000372136

Structurei

Secondary structure

194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi66 – 72Combined sources7
Beta strandi79 – 85Combined sources7
Beta strandi88 – 93Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KHTNMR-A65-94[»]
2PM1X-ray1.60A65-94[»]
3GNYX-ray1.56A/B65-94[»]
3H6CX-ray1.63A/B65-94[»]
3HJ2X-ray1.40A/B65-94[»]
3HJDX-ray1.65A/B65-94[»]
3LO1X-ray1.60A65-94[»]
3LO2X-ray1.56A/B65-94[»]
3LO4X-ray1.75A/B65-94[»]
3LO6X-ray1.56A/B65-94[»]
3LO9X-ray1.56A/B65-94[»]
3LOEX-ray1.56A65-94[»]
3LVXX-ray1.63A/B65-94[»]
4DU0X-ray1.90A/B/C/D65-94[»]
4LB1X-ray2.00A/B/D/E65-94[»]
4LB7X-ray1.90A/B/D/E65-94[»]
4LBBX-ray1.72A/B65-94[»]
4LBFX-ray1.70A/B/C/D/E/F/G/H65-94[»]
ProteinModelPortaliP59665
SMRiP59665
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59665

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JK0K Eukaryota
ENOG41116GP LUCA
GeneTreeiENSGT00730000111183
HOGENOMiHOG000233351
HOVERGENiHBG011703
InParanoidiP59665
KOiK05230
OMAiRVWAFCC
OrthoDBiEOG091G12GE
PhylomeDBiP59665
TreeFamiTF338414

Family and domain databases

InterProiView protein in InterPro
IPR006081 Alpha-defensin
IPR016327 Alpha-defensin_pro
IPR006080 Defensin_beta/alpha
IPR002366 Defensin_propep
PANTHERiPTHR11876 PTHR11876, 1 hit
PfamiView protein in Pfam
PF00323 Defensin_1, 1 hit
PF00879 Defensin_propep, 1 hit
PIRSFiPIRSF001875 Alpha-defensin, 1 hit
SMARTiView protein in SMART
SM00048 DEFSN, 1 hit
PROSITEiView protein in PROSITE
PS00269 DEFENSIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLAILAAI LLVALQAQAE PLQARADEVA AAPEQIAADI PEVVVSLAWD
60 70 80 90
ESLAPKHPGS RKNMACYCRI PACIAGERRY GTCIYQGRLW AFCC
Length:94
Mass (Da):10,201
Last modified:April 30, 2003 - v1
Checksum:i0E0F8E95737396FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21130 mRNA Translation: AAA52302.1
M26602 mRNA Translation: AAA52303.1
L12690 Genomic DNA Translation: AAA36382.1
X52053 mRNA Translation: CAA36280.1
AF200455 Genomic DNA Translation: AAT68875.1
AF200455 Genomic DNA Translation: AAT68878.1
AF200455 Genomic DNA Translation: AAT68879.1
AF200455 Genomic DNA Translation: AAT68880.1
AF238378 Genomic DNA Translation: AAT68883.1
AF238378 Genomic DNA Translation: AAT68884.1
BC069423 mRNA Translation: AAH69423.1
BC093791 mRNA Translation: AAH93791.1
BC112188 mRNA Translation: AAI12189.1
CCDSiCCDS34797.1
CCDS43691.1
PIRiS32499 A40499
RefSeqiNP_001035965.1, NM_001042500.1
NP_004075.1, NM_004084.3
UniGeneiHs.380781

Genome annotation databases

EnsembliENST00000382679; ENSP00000372126; ENSG00000206047
ENST00000382689; ENSP00000372136; ENSG00000240247
ENST00000382692; ENSP00000372139; ENSG00000206047
GeneIDi1667
728358
KEGGihsa:1667
hsa:728358
UCSCiuc003wqv.2 human

Similar proteinsi

Entry informationi

Entry nameiDEF1_HUMAN
AccessioniPrimary (citable) accession number: P59665
Secondary accession number(s): P11479, Q14125, Q6EZF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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