P59663 (MASZ_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	SF3015, S3216

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00641
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 723	722	Malate synthase G HAMAP-Rule MF_00641		PRO_0000166900
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59663 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 048288575D389BF0	FASTA	723	80,362
	10 20 30 40 50 60 MSQTITQGRL RIDANFKRFV DGEVLPGVEL DAAAFWHNVD EIVHDLAPEN RQLLAERDRI 70 80 90 100 110 120 QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV ETTGIDSEIT SQAGPQLVVP 130 140 150 160 170 180 AMNARYALNA ANARWGSLYD ALYGSDIIPQ EGAMVSGYDP QRGEQVIAWV RRFLDESLPL 190 200 210 220 230 240 ENGSYQDVVA FKVVDKQLRI QLKNGKETTL RTPAQFVGYR GDAAAPTCIL LKNNGLHIEL 250 260 270 280 290 300 QIDANGRIGK DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 310 320 330 340 350 360 KMEKNGRQIV RKLNDDRQYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW DSEGNEIPEG 370 380 390 400 410 420 ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA FANKLFTRIE TMLGMAPNTL 430 440 450 460 470 480 KMGIMDEERR TSLNLRSCIA QARNRVAFIN TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP 490 500 510 520 530 540 WIKAYERNNV LSGLFCGLRG KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA 550 560 570 580 590 600 ATLHALHYHH TNVQSVQANI AQTEFSAEFE PLLDDLLTIP VAENANWSAQ EIQQELDNNV 610 620 630 640 650 660 QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI LTKEQVQASL 670 680 690 700 710 720 ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG VKQPNGYTEP LLHAWRLREK ESH

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN44496.1. AE014073 Genomic DNA. Translation: AAP18307.1.
RefSeq	NP_708789.1. NC_004337.2. NP_838497.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P59663.
SMR	P59663. Positions 1-723.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF3015.
Proteomic databases
PaxDb	P59663.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN44496; AAN44496; SF3015. AAP18307; AAP18307; S3216.
GeneID	1026622. 1079471.
KEGG	sfl:SF3015. sfx:S3216.
PATRIC	18708149. VBIShiFle31049_3523.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	SQFIENE.
ProtClustDB	PRK02999.
Enzyme and pathway databases
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_SHIFL

Accession

Primary (citable) accession number: P59663

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program