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P59655

- DHPS_STRR6

UniProt

P59655 - DHPS_STRR6

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Protein

Dihydropteroate synthase

Gene

sulA

Organism
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.1 Publication

Catalytic activityi

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171MagnesiumBy similarity
Binding sitei25 – 251Substrate
Binding sitei91 – 911Substrate
Binding sitei110 – 1101Substrate
Binding sitei201 – 2011Substrate
Binding sitei237 – 2371Substrate
Binding sitei282 – 2821Substrate
Binding sitei284 – 2841Substrate

GO - Molecular functioni

  1. dihydropteroate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. response to antibiotic Source: UniProtKB-KW
  3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSPNE171101:GJC8-273-MONOMER.
UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:sulA
Ordered Locus Names:spr0266
OrganismiStreptococcus pneumoniae (strain ATCC BAA-255 / R6)
Taxonomic identifieri171101 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000586: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Dihydropteroate synthasePRO_0000168231Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi171101.spr0266.

Structurei

Secondary structure

1
314
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 177Combined sources
Helixi31 – 4414Combined sources
Beta strandi48 – 536Combined sources
Helixi63 – 8321Combined sources
Beta strandi87 – 915Combined sources
Helixi95 – 1039Combined sources
Beta strandi108 – 1114Combined sources
Turni112 – 1154Combined sources
Helixi121 – 1288Combined sources
Beta strandi131 – 1355Combined sources
Helixi138 – 1414Combined sources
Turni146 – 1505Combined sources
Helixi166 – 1705Combined sources
Helixi173 – 19119Combined sources
Helixi195 – 1973Combined sources
Beta strandi198 – 2014Combined sources
Helixi210 – 2189Combined sources
Helixi220 – 2245Combined sources
Turni225 – 2273Combined sources
Beta strandi230 – 2334Combined sources
Helixi238 – 2469Combined sources
Helixi256 – 27520Combined sources
Beta strandi279 – 2846Combined sources
Helixi286 – 30015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VEFX-ray1.80A/B1-314[»]
2VEGX-ray2.40A/B1-314[»]
ProteinModelPortaliP59655.
SMRiP59655. Positions 7-303.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59655.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 294285Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 582Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DHPS family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0294.
HOGENOMiHOG000217509.
KOiK00796.
OMAiSALAGWH.
OrthoDBiEOG67T5P5.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59655-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSKANHAKT VICGIINVTP DSFSDGGQFF ALEQALQQAR KLIAEGASML
60 70 80 90 100
DIGGESTRPG SSYVEIEEEI QRVVPVIKAI RKESDVLISI DTWKSQVAEA
110 120 130 140 150
ALAAGADLVN DITGLMGDEK MPHVVAEARA QVVIMFNPVM ARPQHPSSLI
160 170 180 190 200
FPHFGFGQAF TEEELADFET LPIEELMEAF FERALARAAE AGIAPENILL
210 220 230 240 250
DPGIGFGLTK KENLLLLRDL DKLHQKGYPI FLGVSRKRFV INILEENGFE
260 270 280 290 300
VNPETELGFR NRDTASAHVT SIAARQGVEV VRVHDVASHR MAVEIASAIR
310
LADEAENLDL KQYK
Length:314
Mass (Da):34,418
Last modified:April 30, 2003 - v1
Checksum:iC7CEDD0C2EDAA061
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007317 Genomic DNA. Translation: AAK99070.1.
RefSeqiNP_357860.1. NC_003098.1.
WP_010976411.1. NC_003098.1.

Genome annotation databases

EnsemblBacteriaiAAK99070; AAK99070; spr0266.
GeneIDi934662.
KEGGispr:spr0266.
PATRICi19700365. VBIStrPne107296_0303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007317 Genomic DNA. Translation: AAK99070.1 .
RefSeqi NP_357860.1. NC_003098.1.
WP_010976411.1. NC_003098.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VEF X-ray 1.80 A/B 1-314 [» ]
2VEG X-ray 2.40 A/B 1-314 [» ]
ProteinModelPortali P59655.
SMRi P59655. Positions 7-303.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 171101.spr0266.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK99070 ; AAK99070 ; spr0266 .
GeneIDi 934662.
KEGGi spr:spr0266.
PATRICi 19700365. VBIStrPne107296_0303.

Phylogenomic databases

eggNOGi COG0294.
HOGENOMi HOG000217509.
KOi K00796.
OMAi SALAGWH.
OrthoDBi EOG67T5P5.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00156 .
BioCyci SPNE171101:GJC8-273-MONOMER.

Miscellaneous databases

EvolutionaryTracei P59655.

Family and domain databases

Gene3Di 3.20.20.20. 1 hit.
InterProi IPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view ]
Pfami PF00809. Pterin_bind. 1 hit.
[Graphical view ]
SUPFAMi SSF51717. SSF51717. 1 hit.
TIGRFAMsi TIGR01496. DHPS. 1 hit.
PROSITEi PS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-255 / R6.
  2. "Dihydropteroate synthase from Streptococcus pneumoniae: structure, ligand recognition and mechanism of sulfonamide resistance."
    Levy C., Minnis D., Derrick J.P.
    Biochem. J. 412:379-388(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH PTERIN-6-YL-METHYL-MONOPHOSPHATE, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiDHPS_STRR6
AccessioniPrimary (citable) accession number: P59655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3