P59644 (PI5PA_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 80.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A EC=3.1.3.56 Alternative name(s): Inositol polyphosphate 5-phosphatase J | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 1003 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles By similarity. |
| Catalytic activity | D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate. 1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate. |
| Subcellular location | Cytoplasm. Note: Predominantly localized to membrane ruffles. Ref.4 |
| Domain | The 5 Arg-Ser-Xaa-Ser-Xaa-Xaa (RSXSXX) motifs may constitute binding sites for the 14-3-3 protein. Ref.4 |
| Sequence similarities | Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1003 | 1003 | Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A | PRO_0000209739 | |||||
Regions | |||||||||
| Region | 422 – 725 | 304 | Catalytic Potential | ||||||
| Region | 726 – 837 | 112 | Required for ruffle localization | ||||||
| Motif | 6 – 11 | 6 | RSXSXX motif 1 | ||||||
| Motif | 346 – 351 | 6 | SH3-binding Potential | ||||||
| Motif | 351 – 356 | 6 | RSXSXX motif 2 | ||||||
| Motif | 871 – 876 | 6 | RSXSXX motif 3 | ||||||
| Motif | 882 – 887 | 6 | RSXSXX motif 4 | ||||||
| Motif | 908 – 913 | 6 | RSXSXX motif 5 | ||||||
| Compositional bias | 50 – 374 | 325 | Pro-rich | ||||||
| Compositional bias | 837 – 934 | 98 | Ser-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 348 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 350 | 1 | Phosphoserine Ref.5 | ||||||
Experimental info | |||||||||
| Sequence conflict | 343 | 1 | P → R in BAC28654. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Diencephalon. |
| [2] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase SKIP localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation." Gurung R., Tan A., Ooms L.M., McGrath M.J., Huysmans R.D., Munday A.D., Prescott M., Whisstock J.C., Mitchell C.A. J. Biol. Chem. 278:11376-11385(2003) [PubMed: 12536145] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION OF DOMAIN REQUIRED FOR MEMBRANE RUFFLE LOCALIZATION. |
| [5] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348 AND SER-350, MASS SPECTROMETRY. Tissue: Melanoma. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK034272 mRNA. Translation: BAC28654.1. AK170114 mRNA. Translation: BAE41572.1. BC131634 mRNA. Translation: AAI31635.1. BC131635 mRNA. Translation: AAI31636.1. CH466574 Genomic DNA. Translation: EDL40427.1. |
| IPI | IPI00173277. |
| RefSeq | NP_766027.2. NM_172439.3. |
| UniGene | Mm.24313. |
3D structure databases | |
| ProteinModelPortal | P59644. |
| SMR | P59644. Positions 417-734. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P59644. |
PTM databases | |
| PhosphoSite | P59644. |
Proteomic databases | |
| PRIDE | P59644. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000044507; ENSMUSP00000046625; ENSMUSG00000034570. |
| GeneID | 170835. |
| KEGG | mmu:170835. |
Organism-specific databases | |
| CTD | 27124. |
| MGI | MGI:2158663. Inpp5j. |
Phylogenomic databases | |
| HOGENOM | HBG125086. |
| HOVERGEN | HBG082135. |
| InParanoid | P59644. |
| OrthoDB | EOG4HDSTN. |
| PhylomeDB | P59644. |
Gene expression databases | |
| ArrayExpress | P59644. |
| Bgee | P59644. |
| Genevestigator | P59644. |
| GermOnline | ENSMUSG00000034570. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. [Graphical view] |
| KO | K01106. |
| Pfam | PF03372. Exo_endo_phos. 1 hit. [Graphical view] |
| SMART | SM00128. IPPc. 1 hit. [Graphical view] |
| SUPFAM | SSF56219. Exo_endo_phos. 1 hit. |
| ProtoNet | Search... |
Other | |
| NextBio | 370509. |
| SOURCE | Search... |
Entry information
| Entry name | PI5PA_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P59644 Secondary accession number(s): Q3TDM9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with