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Protein

Protein 7a

Gene

7a

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-structural protein which is dispensable for virus replication in cell culture.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

G0/G1 host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 7a
Alternative name(s):
Accessory protein 7a
Protein U122
Protein X4
Gene namesi
ORF Names:7a
OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Taxonomic identifieri227859 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]
Proteomesi
  • UP000000354 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 9681Virion surfaceSequence analysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Topological domaini118 – 1225IntravirionSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 185SCELY → LLLLL: Complete loss of signal sequence cleavage. 1 Publication
Mutagenesisi118 – 1203KRK → ERE: Induces rapid exit from the endoplasmic reticulum. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Chaini16 – 122107Protein 7aPRO_0000037435Add
BLAST

Proteomic databases

PRIDEiP59635.

Interactioni

Subunit structurei

Interacts with the spike glycoprotein, M protein, E protein and the accessory protein 3a. Interacts with human SGT.3 Publications

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 249Combined sources
Beta strandi27 – 326Combined sources
Beta strandi34 – 374Combined sources
Beta strandi39 – 446Combined sources
Turni50 – 523Combined sources
Beta strandi53 – 575Combined sources
Beta strandi60 – 667Combined sources
Beta strandi72 – 809Combined sources
Beta strandi82 – 843Combined sources
Beta strandi89 – 935Combined sources
Beta strandi95 – 973Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XAKX-ray1.80A14-96[»]
1YO4NMR-A16-99[»]
ProteinModelPortaliP59635.
SMRiP59635. Positions 16-99.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59635.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi118 – 1225Di-lysine motif

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.60.40.1550. 1 hit.
InterProiIPR014888. SARS_X4.
[Graphical view]
PfamiPF08779. SARS_X4. 1 hit.
[Graphical view]
SUPFAMiSSF117066. SSF117066. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIILFLTLI VFTSCELYHY QECVRGTTVL LKEPCPSGTY EGNSPFHPLA
60 70 80 90 100
DNKFALTCTS THFAFACADG TRHTYQLRAR SVSPKLFIRQ EEVQQELYSP
110 120
LFLIVAALVF LILCFTIKRK TE
Length:122
Mass (Da):13,941
Last modified:April 23, 2003 - v1
Checksum:i6D8D8D949B0DB4B5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti38 – 381G → A in strain: Isolate Shanghai LY.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA. Translation: AAP13449.1.
AY274119 Genomic RNA. Translation: AAP41043.1.
AY282752 Genomic RNA. No translation available.
AY278554 Genomic RNA. Translation: AAP13573.1.
AY278491 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30036.1.
AY278489 Genomic RNA. Translation: AAP51233.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37023.1.
AY310120 Genomic RNA. Translation: AAP50491.1.
AY291315 Genomic RNA. Translation: AAP33703.1.
AY338174 Genomic RNA. Translation: AAQ01603.1.
AY338175 Genomic RNA. Translation: AAQ01615.1.
AY348314 Genomic RNA. Translation: AAP97888.1.
AP006557 Genomic RNA. Translation: BAC81354.1.
AP006558 Genomic RNA. Translation: BAC81368.1.
AP006559 Genomic RNA. Translation: BAC81382.1.
AP006560 Genomic RNA. Translation: BAC81396.1.
AP006561 Genomic RNA. Translation: BAC81410.1.
AY323977 Genomic RNA. Translation: AAP72980.1.
AY427439 Genomic RNA. Translation: AAQ94066.1.
AY463059 Genomic RNA. Translation: AAP82982.2.
AY322208 Genomic RNA. Translation: AAP82970.1.
RefSeqiNP_828857.1. NC_004718.3.

Genome annotation databases

GeneIDi1489674.
KEGGivg:1489674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA. Translation: AAP13449.1.
AY274119 Genomic RNA. Translation: AAP41043.1.
AY282752 Genomic RNA. No translation available.
AY278554 Genomic RNA. Translation: AAP13573.1.
AY278491 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30036.1.
AY278489 Genomic RNA. Translation: AAP51233.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37023.1.
AY310120 Genomic RNA. Translation: AAP50491.1.
AY291315 Genomic RNA. Translation: AAP33703.1.
AY338174 Genomic RNA. Translation: AAQ01603.1.
AY338175 Genomic RNA. Translation: AAQ01615.1.
AY348314 Genomic RNA. Translation: AAP97888.1.
AP006557 Genomic RNA. Translation: BAC81354.1.
AP006558 Genomic RNA. Translation: BAC81368.1.
AP006559 Genomic RNA. Translation: BAC81382.1.
AP006560 Genomic RNA. Translation: BAC81396.1.
AP006561 Genomic RNA. Translation: BAC81410.1.
AY323977 Genomic RNA. Translation: AAP72980.1.
AY427439 Genomic RNA. Translation: AAQ94066.1.
AY463059 Genomic RNA. Translation: AAP82982.2.
AY322208 Genomic RNA. Translation: AAP82970.1.
RefSeqiNP_828857.1. NC_004718.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XAKX-ray1.80A14-96[»]
1YO4NMR-A16-99[»]
ProteinModelPortaliP59635.
SMRiP59635. Positions 16-99.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP59635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489674.
KEGGivg:1489674.

Miscellaneous databases

EvolutionaryTraceiP59635.

Family and domain databases

Gene3Di2.60.40.1550. 1 hit.
InterProiIPR014888. SARS_X4.
[Graphical view]
PfamiPF08779. SARS_X4. 1 hit.
[Graphical view]
SUPFAMiSSF117066. SSF117066. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Urbani.
  2. "The genome sequence of the SARS-associated coronavirus."
    Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H.
    , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
    Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Tor2.
  3. "Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
    Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
    N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
  4. "The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
    Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
    Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HKU-39849.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
  6. "The complete genome of SARS coronavirus clone TW1."
    Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TW1.
  7. "SARS virus is a close relative of type II coronaviruses."
    Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate FRA.
  8. Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S., Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Frankfurt 1.
  9. Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
  10. "The complete genome of SARS coronavirus TWH."
    Shu H.Y., Wu K.M., Tsai S.F.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
  11. Canducci F., Clementi M., Poli G., Vicenzi E.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HSR 1.
  12. Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate AS.
  13. "Analysis of SARS coronavirus genome in Shanghai isolates."
    Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Shanghai QXC1.
  14. Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 32-122.
    Strain: Isolate Shanghai LY.
  15. "Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein."
    Nelson C.A., Pekosz A., Lee C.A., Diamond M.S., Fremont D.H.
    Structure 13:75-85(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PEPTIDE SEQUENCE OF 16-29, SUBCELLULAR LOCATION, X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 16-96.
  16. "A novel severe acute respiratory syndrome coronavirus protein, U274, is transported to the cell surface and undergoes endocytosis."
    Tan Y.-J., Teng E., Shen S., Tan T.H.P., Goh P.-Y., Fielding B.C., Ooi E.-E., Tan H.-C., Lim S.G., Hong W.
    J. Virol. 78:6723-6734(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ACCESSORY PROTEIN 3A.
  17. "Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus."
    Fielding B.C., Tan Y.-J., Shuo S., Tan T.H.P., Ooi E.-E., Lim S.G., Hong W., Goh P.-Y.
    J. Virol. 78:7311-7318(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DI-LYSINE MOTIF, MUTAGENESIS OF 14-SER--TYR-18 AND 118-LYS--LYS-120.
  18. "Overexpression of 7a, a protein specifically encoded by the severe acute respiratory syndrome coronavirus, induces apoptosis via a caspase-dependent pathway."
    Tan Y.-J., Fielding B.C., Goh P.-Y., Shen S., Tan T.H.P., Lim S.G., Hong W.
    J. Virol. 78:14043-14047(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Severe acute respiratory syndrome coronavirus 7a accessory protein is a viral structural protein."
    Huang C., Ito N., Tseng C.-T.K., Makino S.
    J. Virol. 80:7287-7294(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPIKE GLYCOPROTEIN.
  20. "Structure, expression, and intracellular localization of the SARS-CoV accessory proteins 7a and 7b."
    Pekosz A., Schaecher S.R., Diamond M.S., Fremont D.H., Sims A.C., Baric R.S.
    Adv. Exp. Med. Biol. 581:115-120(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  21. "Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT."
    Fielding B.C., Gunalan V., Tan T.H.P., Chou C.-F., Shen S., Khan S., Lim S.G., Hong W., Tan Y.-J.
    Biochem. Biophys. Res. Commun. 343:1201-1208(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH M PROTEIN; E PROTEIN AND HUMAN SGT.
  22. "Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains."
    Haenel K., Stangler T., Stoldt M., Willbold D.
    J. Biomed. Sci. 13:281-293(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 16-99.

Entry informationi

Entry nameiNS7A_CVHSA
AccessioniPrimary (citable) accession number: P59635
Secondary accession number(s): Q7TA11, Q7TA17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: April 13, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.