P59633 (NS3B_CVHSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-structural protein 3b
Short name=ns3b

Alternative name(s):
Accessory protein 3b
Protein X2

Gene names

ORF Names:

3b

Organism

Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus) [Reference proteome]

Taxonomic identifier

227859 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus › Severe acute respiratory syndrome-related coronavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Induces host cell G0/G1 arrest and apoptosis. Ref.14 Ref.16
Subcellular location	Host nucleus › host nucleolus. Host mitochondrion Ref.15 Ref.16.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Host mitochondrion Host nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Non-structural protein 3b		PRO_0000106132
Regions
	Region	80 – 138	59	Mitochondrial targeting signal
	Region	134 – 154	21	Nucleolar targeting
	Motif	135 – 153	19	Bipartite nuclear localization signal Potential
Natural variations
	Natural variant	31	1	K → Q in strain: Isolate GD01.
	Natural variant	99	1	T → I in strain: Isolate BJ02.
	Natural variant	115	1	L → Q in strain: Isolate GD01.
	Natural variant	121	1	Q → P in strain: Isolate BJ01 and Isolate BJ04.

Sequences

	Sequence		Length	Mass (Da)
	P59633 [UniParc]. Last modified April 23, 2003. Version 1. Checksum: 82AB0B68F11060A8	FASTA	154	17,750
	10 20 30 40 50 60 MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM 70 80 90 100 110 120 SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI 130 140 150 QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR

References

[1]	"Characterization of a novel coronavirus associated with severe acute respiratory syndrome." Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S., Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D. , Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J., Bellini W.J. Science 300:1394-1399(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Urbani.
[2]	"The genome sequence of the SARS-associated coronavirus." Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H. , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L. Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Tor2.
[3]	"Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome." Tsui S.K.W., Chim S.S.C., Lo Y.M.D. N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
[4]	"The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)." Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C. Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HKU-39849.
[5]	Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F. , Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W., Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W., Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L., Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J., Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
[6]	"The complete genome of SARS coronavirus clone TW1." Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TW1.
[7]	"SARS virus is a close relative of type II coronaviruses." Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate FRA.
[8]	Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S., Scheiner B., Weinand H., Weissbrich B., Ziebuhr J. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Frankfurt 1.
[9]	"Analysis of SARS coronavirus genome in Shanghai isolates." Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Shanghai QXC1.
[10]	Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
[11]	"The complete genome of SARS coronavirus TWH." Shu H.Y., Wu K.M., Tsai S.F. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
[12]	Canducci F., Clementi M., Poli G., Vicenzi E. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HSR 1.
[13]	Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate AS.
[14]	"G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells." Yuan X., Shan Y., Zhao Z., Chen J., Cong Y. Virol. J. 2:66-66(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus." Yuan X., Yao Z., Shan Y., Chen B., Yang Z., Wu J., Zhao Z., Chen J., Cong Y. Virus Res. 114:70-79(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[16]	"Mitochondrial location of severe acute respiratory syndrome coronavirus 3b protein." Yuan X., Shan Y., Yao Z., Li J., Zhao Z., Chen J., Cong Y. Mol. Cells 21:186-191(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY278741 Genomic RNA. Translation: AAP13447.1. AY274119 Genomic RNA. Translation: AAP41039.1. AY282752 Genomic RNA. No translation available. AY278554 Genomic RNA. Translation: AAP13569.1. AY278491 Genomic RNA. No translation available. AY278487 Genomic RNA. No translation available. AY278488 Genomic RNA. Translation: AAP30032.1. AY278489 Genomic RNA. Translation: AAP51229.1. AY278490 Genomic RNA. No translation available. AY279354 Genomic RNA. No translation available. AY291451 Genomic RNA. Translation: AAP37019.1. AY310120 Genomic RNA. Translation: AAP50487.1. AY291315 Genomic RNA. Translation: AAP33699.1. AY463059 Genomic RNA. Translation: AAR86789.1. AY338174 Genomic RNA. Translation: AAQ01599.1. AY338175 Genomic RNA. Translation: AAQ01611.1. AY348314 Genomic RNA. Translation: AAP97884.1. AP006557 Genomic RNA. Translation: BAC81350.1. AP006558 Genomic RNA. Translation: BAC81364.1. AP006559 Genomic RNA. Translation: BAC81378.1. AP006560 Genomic RNA. Translation: BAC81392.1. AP006561 Genomic RNA. Translation: BAC81406.1. AY323977 Genomic RNA. Translation: AAP72976.1. AY427439 Genomic RNA. Translation: AAQ94062.1.
RefSeq	NP_828853.1. NC_004718.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489670.
Family and domain databases
InterPro	IPR022117. SARS_3b. [Graphical view]
Pfam	PF12383. SARS_3b. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NS3B_CVHSA

Accession

Primary (citable) accession number: P59633
Secondary accession number(s): Q7TFA9, Q80BV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program