Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P59633 (NS3B_CVHSA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3b

Short name=ns3b
Alternative name(s):
Accessory protein 3b
Protein X2
Gene names
ORF Names:3b
OrganismHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus) [Reference proteome]
Taxonomic identifier227859 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Induces host cell G0/G1 arrest and apoptosis. Ref.14 Ref.16

Subcellular location

Host nucleushost nucleolus. Host mitochondrion Ref.15 Ref.16.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentHost mitochondrion
Host nucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cytokine production

Inferred from expression pattern PubMed 21561061. Source: CACAO

   Cellular_componenthost cell mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell nucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154Non-structural protein 3b
PRO_0000106132

Regions

Region80 – 13859Mitochondrial targeting signal
Region134 – 15421Nucleolar targeting
Motif135 – 15319Bipartite nuclear localization signal Potential

Natural variations

Natural variant311K → Q in strain: Isolate GD01.
Natural variant991T → I in strain: Isolate BJ02.
Natural variant1151L → Q in strain: Isolate GD01.
Natural variant1211Q → P in strain: Isolate BJ01 and Isolate BJ04.

Sequences

Sequence LengthMass (Da)Tools
P59633 [UniParc].

Last modified April 23, 2003. Version 1.
Checksum: 82AB0B68F11060A8

FASTA15417,750
        10         20         30         40         50         60 
MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM 

        70         80         90        100        110        120 
SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI 

       130        140        150 
QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR 

« Hide

References

[1]"Characterization of a novel coronavirus associated with severe acute respiratory syndrome."
Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S., Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D. expand/collapse author list , Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J., Bellini W.J.
Science 300:1394-1399(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Urbani.
[2]"The genome sequence of the SARS-associated coronavirus."
Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H. expand/collapse author list , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Tor2.
[3]"Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
[4]"The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HKU-39849.
[5]Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F. expand/collapse author list , Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W., Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W., Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L., Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J., Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
[6]"The complete genome of SARS coronavirus clone TW1."
Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TW1.
[7]"SARS virus is a close relative of type II coronaviruses."
Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate FRA.
[8]Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S., Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Frankfurt 1.
[9]"Analysis of SARS coronavirus genome in Shanghai isolates."
Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Shanghai QXC1.
[10]Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
[11]"The complete genome of SARS coronavirus TWH."
Shu H.Y., Wu K.M., Tsai S.F.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
[12]Canducci F., Clementi M., Poli G., Vicenzi E.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HSR 1.
[13]Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate AS.
[14]"G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells."
Yuan X., Shan Y., Zhao Z., Chen J., Cong Y.
Virol. J. 2:66-66(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus."
Yuan X., Yao Z., Shan Y., Chen B., Yang Z., Wu J., Zhao Z., Chen J., Cong Y.
Virus Res. 114:70-79(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[16]"Mitochondrial location of severe acute respiratory syndrome coronavirus 3b protein."
Yuan X., Shan Y., Yao Z., Li J., Zhao Z., Chen J., Cong Y.
Mol. Cells 21:186-191(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY278741 Genomic RNA. Translation: AAP13447.1.
AY274119 Genomic RNA. Translation: AAP41039.1.
AY282752 Genomic RNA. No translation available.
AY278554 Genomic RNA. Translation: AAP13569.1.
AY278491 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30032.1.
AY278489 Genomic RNA. Translation: AAP51229.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37019.1.
AY310120 Genomic RNA. Translation: AAP50487.1.
AY291315 Genomic RNA. Translation: AAP33699.1.
AY463059 Genomic RNA. Translation: AAR86789.1.
AY338174 Genomic RNA. Translation: AAQ01599.1.
AY338175 Genomic RNA. Translation: AAQ01611.1.
AY348314 Genomic RNA. Translation: AAP97884.1.
AP006557 Genomic RNA. Translation: BAC81350.1.
AP006558 Genomic RNA. Translation: BAC81364.1.
AP006559 Genomic RNA. Translation: BAC81378.1.
AP006560 Genomic RNA. Translation: BAC81392.1.
AP006561 Genomic RNA. Translation: BAC81406.1.
AY323977 Genomic RNA. Translation: AAP72976.1.
AY427439 Genomic RNA. Translation: AAQ94062.1.
RefSeqNP_828853.1. NC_004718.3.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1489670.

Family and domain databases

InterProIPR022117. SARS_3b.
[Graphical view]
PfamPF12383. SARS_3b. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNS3B_CVHSA
AccessionPrimary (citable) accession number: P59633
Secondary accession number(s): Q7TFA9, Q80BV4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: March 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program