Reviewed, UniProtKB/Swiss-Prot P59626 (PYRDA_ENTFA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase A
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase A
    DHOdehase A
      Short name=DHODase A
      Short name=DHOD A

Gene names

Name:	pyrDA
Synonyms:	pydA, pyrD-1
Ordered Locus Names:	EF_0285

Organism

Enterococcus faecalis (Streptococcus faecalis) [Complete proteome] [HAMAP]

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Dihydroorotate dehydrogenase A		PRO_0000148390
Sites
	Active site	130	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59626-1 [UniParc]. Last modified April 23, 2003. Version 1. Checksum: A534C74755E914CD	FASTA	311	34,477
	10 20 30 40 50 60 MDISVEFSGH KLANVLMNAS GIHCMTIKEM DELAASQAGA FVAKTATPNP RQGNEEPRYF 70 80 90 100 110 120 DTPLGSINSM GLPNLGIDYY LDYQIARQKE FPEELRFLSV SGMNYEENIA ILKKVQESEY 130 140 150 160 170 180 TGVTEFNLSC PNLPGKPQIA YDFELTEKLL TEVFQFFTKP LGVKLPPFFD IAHFDAMAEI 190 200 210 220 230 240 LNKFPLVYVN SINSIGNGLY IDSDKEEVVI KPKGGFGGLG GEYVKPTALA NVRAFAQRLK 250 260 270 280 290 300 PEIKIIGTGG ITCGKDVFEH LLCGATLVQV GTQLHQEGPQ VFERLAKELQ EIMAAKGYES 310 IEEFRGKLKE M

References

[1]

"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: V583 / ATCC 700802.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016830 Genomic DNA. Translation: AAO80148.1.
RefSeq	NP_814077.1.
3D structure databases
HSSP	HSSP built from PDB template 2DOR based on UniProtKB P54321.
ModBase	Search...
Genome annotation databases
GeneID	1199203.
GenomeReviews	Gene locus EF_0285 in contig AE016830_GR.
KEGG	efa:EF0285.
NMPDR	fig|226185.1.peg.263.
TIGR	EF_0285.
Phylogenomic databases
HOGENOM	P59626.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0285-MON.
Family and domain databases
HAMAP	MF_00224. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005720. DHO_DHase_1_core. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRDA_ENTFA

Accession

Primary (citable) accession number: P59626

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)