Reviewed, UniProtKB/Swiss-Prot P59610 (ARGJ_BRAJA)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	blr0206

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000002135
	Chain	195 – 413	219	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000002136
Sites
	Site	194 – 195	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59610-1 [UniParc]. Last modified April 23, 2003. Version 1. Checksum: F5B3BE3F558DCF33	FASTA	413	42,683
	10 20 30 40 50 60 MSSSVSPLAP KTVPDMPVIA GVRLATAEAG IRYKNRTDVL LAVMDKGTAV AGVFTKSKCP 70 80 90 100 110 120 SAPVEWCRAK LKGGKARALV VNSGNANAFT GKTGRSSTAL TAKIAAKAVG CSESEIFLAS 130 140 150 160 170 180 TGVIGEPLDA TKFDGVLGRL AETAEPGDYL AAAKAIMTTD TFPKVATATV KLGKAKVTIN 190 200 210 220 230 240 GMAKGAGMIA PDMATMLSFV FTDAPIAPAA LQALLKSGVE DTFNAVTIDG DTSTSDTLLA 250 260 270 280 290 300 FATGAAAEHG APKISRASDP RLKAFVKAFN QVLANLAEQV ARDGEGARKL VEITVEGAKT 310 320 330 340 350 360 KASARKIAMS IANSPLVKTA IAGEDANWGR VVMAVGKAGE PADRDKLSIS FNGIRVARSG 370 380 390 400 410 ARDPDYDEAQ VSEAMKAPEI AIKVSLGLGK GRDRVMTCDL TKEYVAINGD YRS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45471.1.
RefSeq	NP_766846.1.
3D structure databases
SMR	P59610. Positions 19-194, 20-410, 195-411.
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	1049177.
GenomeReviews	Gene locus blr0206 in contig BA000040_GR.
KEGG	bja:blr0206.
NMPDR	fig|224911.1.peg.206.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284202.
OMA	QNRFCAA.
PhylomeDB	P59610.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0206-MONOMER.
BRENDA	2.3.1.1. 280. 2.3.1.35. 280.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_BRAJA

Accession

Primary (citable) accession number: P59610

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)