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Protein

Argininosuccinate synthase

Gene

argG

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei99 – 991CitrullineBy similarity
Binding sitei129 – 1291ATP; via amide nitrogenBy similarity
Binding sitei131 – 1311AspartateBy similarity
Binding sitei131 – 1311ATPBy similarity
Binding sitei135 – 1351AspartateBy similarity
Binding sitei135 – 1351CitrullineBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361ATPBy similarity
Binding sitei139 – 1391CitrullineBy similarity
Binding sitei192 – 1921CitrullineBy similarity
Binding sitei194 – 1941ATPBy similarity
Binding sitei201 – 2011CitrullineBy similarity
Binding sitei203 – 2031CitrullineBy similarity
Binding sitei280 – 2801CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-525-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:bll0522
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Argininosuccinate synthasePRO_0000148693Add
BLAST

Proteomic databases

PRIDEiP59607.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224911.bll0522.

Structurei

3D structure databases

ProteinModelPortaliP59607.
SMRiP59607. Positions 2-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
InParanoidiP59607.
KOiK01940.
OrthoDBiEOG6K9QCV.
PhylomeDBiP59607.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59607-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTILKSLPK GEKVGIAFSG GLDTSAALLW MKQKGARCYA YTANLGQPDE
60 70 80 90 100
ADYNEIPRKA QEFGAEKAVL VDCRTQLVHE GIAAIQSGAF HISTGGITYF
110 120 130 140 150
NTTPLGRAVT GTMLVAAMKE DGVNIWGDGS TFKGNDIERF YRYGLLTNPS
160 170 180 190 200
LRIYKPWLDQ QFIDELGGRA EMSAFMTAQG FAYKMSAEKA YSTDSNLLGA
210 220 230 240 250
THEAKDLESL DSGIKIVNPI MGVPFWREDC NVKAEKVVVR FEEGQPTALN
260 270 280 290 300
GQTFTDPVAL FLEANAIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HIAYERLVTG IHNEDTIEQY RISGMRLGRL LYQGRWFDSQ ALMLRETAQR
360 370 380 390 400
WVARAVTGEV TLELRRGNDY SILNTESPNL TYAPERLSME KVEDAAFTPA
410 420 430 440
DRIGQLTMRN LDIADTRTKL KLYTDTGLLS GSEGSQIFRL ENDKG
Length:445
Mass (Da):49,231
Last modified:April 23, 2003 - v1
Checksum:i7A5112563C7A8529
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC45787.1.
RefSeqiNP_767162.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC45787; BAC45787; BAC45787.
GeneIDi1049678.
KEGGibja:bll0522.
PATRICi21184521. VBIBraJap65052_0538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC45787.1.
RefSeqiNP_767162.1. NC_004463.1.

3D structure databases

ProteinModelPortaliP59607.
SMRiP59607. Positions 2-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll0522.

Proteomic databases

PRIDEiP59607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC45787; BAC45787; BAC45787.
GeneIDi1049678.
KEGGibja:bll0522.
PATRICi21184521. VBIBraJap65052_0538.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
InParanoidiP59607.
KOiK01940.
OrthoDBiEOG6K9QCV.
PhylomeDBiP59607.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciBJAP224911:GJEJ-525-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiASSY_BRADU
AccessioniPrimary (citable) accession number: P59607
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.