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P59598

- ASXL1_MOUSE

UniProt

P59598 - ASXL1_MOUSE

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Protein

Putative Polycomb group protein ASXL1

Gene

Asxl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG). Acts as a coactivator of RARA and RXRA through association with NCOA1. Acts as a corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner; the function seems to involve differential recruitment of methylated histone H3 to respective promoters. Acts as a corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1476 – 151338PHD-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. peroxisome proliferator activated receptor binding Source: UniProtKB
  4. retinoic acid receptor binding Source: UniProtKB
  5. transcription coactivator activity Source: UniProtKB
  6. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. bone development Source: MGI
  2. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  3. negative regulation of fat cell differentiation Source: UniProtKB
  4. negative regulation of peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
  5. negative regulation of retinoic acid receptor signaling pathway Source: UniProtKB
  6. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. positive regulation of retinoic acid receptor signaling pathway Source: UniProtKB
  8. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  9. response to retinoic acid Source: UniProtKB
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Putative Polycomb group protein ASXL1
Alternative name(s):
Additional sex combs-like protein 1
Gene namesi
Name:Asxl1
Synonyms:Kiaa0978
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2684063. Asxl1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. nuclear chromatin Source: UniProtKB
  3. PR-DUB complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi170 – 1701P → A: Abolishes interaction with CBX1, CBX3 and CBX5; when associated with A-172 and A-174. 1 Publication
Mutagenesisi172 – 1721V → A: Abolishes interaction with CBX1, CBX3 and CBX5; when associated with A-170 and A-174. 1 Publication
Mutagenesisi174 – 1741L → A: Abolishes interaction with CBX1, CBX3 and CBX5; when associated with A-170 and A-172. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15141514Putative Polycomb group protein ASXL1PRO_0000059322Add
BLAST

Proteomic databases

PaxDbiP59598.
PRIDEiP59598.

PTM databases

PhosphoSiteiP59598.

Expressioni

Gene expression databases

BgeeiP59598.
CleanExiMM_ASXL1.
ExpressionAtlasiP59598. baseline and differential.
GenevestigatoriP59598.

Interactioni

Subunit structurei

Component of the PR-DUB complex, at least composed of BAP1 and ASXL1 (By similarity). Interacts with RARA, RXRA, NCOA1, KDM1A and CBX5. Interacts with PPARA, PPARG, CBX1 and CBX3.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBX5P459735EBI-5743705,EBI-78219From a different organism.
ESR1P033722EBI-5743705,EBI-78473From a different organism.
KDM1AO603412EBI-5743705,EBI-710124From a different organism.
NCOA1Q157882EBI-5743705,EBI-455189From a different organism.
NR3C1P041502EBI-5743705,EBI-493507From a different organism.
RARAP102764EBI-5743705,EBI-413374From a different organism.
RxraP287002EBI-5743705,EBI-346715

Protein-protein interaction databases

BioGridi230772. 3 interactions.
IntActiP59598. 12 interactions.

Structurei

3D structure databases

ProteinModelPortaliP59598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 1745Required for interaction with CBX5
Regioni175 – 370196Interaction with KDM1AAdd
BLAST
Regioni300 – 655356Interaction with NCOA1Add
BLAST
Regioni1082 – 10876Required for interaction with RARABy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi284 – 2885LXXLL motif 1
Motifi409 – 4135Nuclear localization signalSequence Analysis
Motifi808 – 8125LXXLL motif 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi199 – 20911Poly-SerAdd
BLAST
Compositional biasi284 – 2885Poly-Leu
Compositional biasi639 – 67032Gly-richAdd
BLAST
Compositional biasi1430 – 14334Poly-Ser

Domaini

Contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which may be required for an association with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the Asx family.Curated
Contains 1 PHD-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1476 – 151338PHD-type; atypicalAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG81527.
HOGENOMiHOG000293301.
HOVERGENiHBG050598.
InParanoidiP59598.
KOiK11471.
OMAiSLKAMIM.
OrthoDBiEOG7SXW2P.
PhylomeDBiP59598.
TreeFamiTF328464.

Family and domain databases

InterProiIPR026905. ASX-like_PHD.
IPR024811. ASX/ASX-like.
IPR028020. ASXH.
IPR024815. ASXL1.
IPR007759. DNA-dir_RNA_pol_delta/Asxl.
[Graphical view]
PANTHERiPTHR13578. PTHR13578. 1 hit.
PTHR13578:SF19. PTHR13578:SF19. 1 hit.
PfamiPF13919. ASXH. 1 hit.
PF05066. HARE-HTH. 1 hit.
PF13922. PHD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59598 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDKQKRKKE RTWAEAARLV LENYSDAPMT PKQILQVIEA EGLKEMRSGT
60 70 80 90 100
SPLACLNAML HSNSRGGEGL FYKLPGRISL FTLKKDAVQW SRNAATVDGD
110 120 130 140 150
EPEDSADVES CGSNEASTVS GENDVSLDET SSNASCSTES QSRPLSNPRD
160 170 180 190 200
SHRASSQANK QKKRTGVMLP RVVLTPLKVN GAHVEPASGF SGRHADGESG
210 220 230 240 250
SPSSSSSGSL ALGNSAIRGQ AEVTRDPAPL LRGFRKPATG QMKRNRGEEV
260 270 280 290 300
DFETPGSILV NTNLRALINS RTFHALPLHF QQQLLLLLPE VDRQVGTDGL
310 320 330 340 350
LRLSGSALNN EFFTHAAQSW RERLADGEFT HEMQVRLRQE MEKEKKVEQW
360 370 380 390 400
KEKFFEDYYG QKLGLTKEES LQQKEVQEEA KVKSGLCVSG ESVRPQRGPN
410 420 430 440 450
TRQRDGHFKK RSRPDLRTRS RRNIYKKQEP EQAGVAKDAS AAPDVSLSKD
460 470 480 490 500
TKTDLAGVNS TPGPDVSSAT SGQEGPKCPS EPVASQIQAE RDNLACASAS
510 520 530 540 550
PDRIPTLPQD TVDQETKDQK RKSFEQEASA SFPEKKPRLE DRQSFRNTIE
560 570 580 590 600
SVHTEKPQPT KEEPKVPPIR IQLSRIKPPW VAKGRPTYQI CPRIVPITES
610 620 630 640 650
SCRGWTGART LADIKARALQ ARGARGYHCN RETATTAIGG GGGPGGGGSG
660 670 680 690 700
AIDEGGGRDS SSGDGSEACG HPEPRGAPST SGESASDLQR TQLLPPCPLN
710 720 730 740 750
GEHTPAEAAM PRARREDSAS LRKEESCLLK RVPGVLTSGL EDASQPPIAP
760 770 780 790 800
TGDQPCQALP PLSSQTPVAE MLTEQPKLLL DDRTECESSR EDQGPTIPSE
810 820 830 840 850
SSSGRFPLGD LLGGGSDQAF DNMKEPVSMT PTFISELSLA NYLQDRPDDD
860 870 880 890 900
GLGLGATGLL IRESSRQEAL TEAFASGSPT SWVPILSNYE VIKTSDPESR
910 920 930 940 950
ENIPCPEPQD EKEWERAVPL IAATESVPQP ESCISHWTPP PAAVGSTGSD
960 970 980 990 1000
SEQVDLERLE MNGISEAPSP HSESTDTASD SEGHLSEDSS EVDASEVTVV
1010 1020 1030 1040 1050
KGSLGGDEKQ DWDPSASLSK VNNDLSVLTR TGGVAASQSW VSRVCSVPHK
1060 1070 1080 1090 1100
IPDSLLLSST ECQPRSVCPL RPGSSVEVTN PLVMHLLHGN LPLEKVLPPG
1110 1120 1130 1140 1150
HRSSRLESSQ LPLREQSQDR GTLQGTGENN RLAARINPGS AQTLKESILA
1160 1170 1180 1190 1200
QSYGASAGLV RAMASKAPAM SQKIAKMVTS LDSQHPETEL TPSSGNLEEI
1210 1220 1230 1240 1250
DSKEHLSSFL CEEQKEGHSL SQGSDPGAAP GQCLGDHTTS KVPCFSSTNV
1260 1270 1280 1290 1300
SLSFGSEQTD GTLSDQNNAG GHEKKLFGPG NTVTTLQCPR SEEQTPLPAE
1310 1320 1330 1340 1350
VPPVFPSRKI EPSKNSVSGG VQTTRENRMP KPPPVSADSI KTEQTFLRDP
1360 1370 1380 1390 1400
IKADAENRKA AGYSSLELVG HLQGMPFVVD LPFWKLPREP GKGFSQPLEP
1410 1420 1430 1440 1450
SSIPSQLNIK QALYGKLSKL QLSPTSFNYS SSSATFPKGL AGGVVQLSHK
1460 1470 1480 1490 1500
ASFGTGHTAS LSLQMFADSS AVESISLQCA CSLKAMIMCQ GCGAFCHDDC
1510
IGPSKLCVLC LVVR
Length:1,514
Mass (Da):162,674
Last modified:April 23, 2003 - v1
Checksum:i20D9FBE9D4CF9E74
GO

Sequence cautioni

The sequence BAC65695.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK122413 mRNA. Translation: BAC65695.1. Different initiation.
CCDSiCCDS38285.1.
RefSeqiNP_001035028.1. NM_001039939.1.
UniGeneiMm.330677.

Genome annotation databases

EnsembliENSMUST00000109790; ENSMUSP00000105413; ENSMUSG00000042548.
GeneIDi228790.
KEGGimmu:228790.
UCSCiuc008nhs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK122413 mRNA. Translation: BAC65695.1 . Different initiation.
CCDSi CCDS38285.1.
RefSeqi NP_001035028.1. NM_001039939.1.
UniGenei Mm.330677.

3D structure databases

ProteinModelPortali P59598.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230772. 3 interactions.
IntActi P59598. 12 interactions.

PTM databases

PhosphoSitei P59598.

Proteomic databases

PaxDbi P59598.
PRIDEi P59598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000109790 ; ENSMUSP00000105413 ; ENSMUSG00000042548 .
GeneIDi 228790.
KEGGi mmu:228790.
UCSCi uc008nhs.1. mouse.

Organism-specific databases

CTDi 171023.
MGIi MGI:2684063. Asxl1.
Rougei Search...

Phylogenomic databases

eggNOGi NOG81527.
HOGENOMi HOG000293301.
HOVERGENi HBG050598.
InParanoidi P59598.
KOi K11471.
OMAi SLKAMIM.
OrthoDBi EOG7SXW2P.
PhylomeDBi P59598.
TreeFami TF328464.

Miscellaneous databases

NextBioi 379148.
PROi P59598.
SOURCEi Search...

Gene expression databases

Bgeei P59598.
CleanExi MM_ASXL1.
ExpressionAtlasi P59598. baseline and differential.
Genevestigatori P59598.

Family and domain databases

InterProi IPR026905. ASX-like_PHD.
IPR024811. ASX/ASX-like.
IPR028020. ASXH.
IPR024815. ASXL1.
IPR007759. DNA-dir_RNA_pol_delta/Asxl.
[Graphical view ]
PANTHERi PTHR13578. PTHR13578. 1 hit.
PTHR13578:SF19. PTHR13578:SF19. 1 hit.
Pfami PF13919. ASXH. 1 hit.
PF05066. HARE-HTH. 1 hit.
PF13922. PHD_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Additional sex comb-like 1 (ASXL1), in cooperation with SRC-1, acts as a ligand-dependent coactivator for retinoic acid receptor."
    Cho Y.S., Kim E.J., Park U.H., Sin H.S., Um S.J.
    J. Biol. Chem. 281:17588-17598(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RARA AND RXRA.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "ASXL1 represses retinoic acid receptor-mediated transcription through associating with HP1 and LSD1."
    Lee S.W., Cho Y.S., Na J.M., Park U.H., Kang M., Kim E.J., Um S.J.
    J. Biol. Chem. 285:18-29(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KDM1A; CBX1; CBX3 AND CBX5, MUTAGENESIS OF PRO-170; VAL-172 AND LEU-174.
  5. "Additional sex comb-like (ASXL) proteins 1 and 2 play opposite roles in adipogenesis via reciprocal regulation of peroxisome proliferator-activated receptor {gamma}."
    Park U.H., Yoon S.K., Park T., Kim E.J., Um S.J.
    J. Biol. Chem. 286:1354-1363(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPARA AND PPARG.

Entry informationi

Entry nameiASXL1_MOUSE
AccessioniPrimary (citable) accession number: P59598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3