P59596 (VME1_CVHSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane protein
Short name=M protein

Alternative name(s):
E1 glycoprotein
Matrix glycoprotein
Membrane glycoprotein

Gene names

Name:	M
ORF Names:	5

Organism

Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus) [Reference proteome]

Taxonomic identifier

227859 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus › Severe acute respiratory syndrome-related coronavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins By similarity.
Subunit structure	Homomultimer. Interacts with envelope E protein in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex. Forms a complex with HE and S proteins. Interacts with nucleocapsid N protein. This interaction probably participates in RNA packaging into the virus By similarity. Interacts with the accessory proteins 3a and 7a. Ref.20 Ref.21 Ref.22
Subcellular location	Virion membrane; Multi-pass membrane protein Potential. Host Golgi apparatus membrane; Multi-pass membrane protein Potential. Note: Largely embedded in the lipid bilayer By similarity.
Sequence similarities	Belongs to the coronaviruses M protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host TRAFs by virus Inhibition of host innate immune response by virus Viral immunoevasion
Cellular component	Host Golgi apparatus Host membrane Membrane Viral envelope protein Viral matrix protein Virion
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host TRAF activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host innate immune response Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW viral assembly, maturation, egress, and release Non-traceable author statement. Source: UniProtKB
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Non-traceable author statement. Source: UniProtKB virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural molecule activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Membrane protein		PRO_0000106035
Regions
	Topological domain	1 – 18	18	Virion surface Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 49	10	Intravirion Potential
	Transmembrane	50 – 70	21	Helical; Potential
	Topological domain	71 – 78	8	Virion surface Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Topological domain	100 – 221	122	Intravirion Potential
Amino acid modifications
	Glycosylation	4	1	N-linked (GlcNAc...); by host Potential
Natural variations
	Natural variant	11	1	E → K in strain: Isolate Sin2500.
	Natural variant	27	1	F → C in strain: Isolate CUHK-Su10, Isolate TWC2, Isolate TWC3, Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS, Isolate TWY, Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
	Natural variant	68	1	A → V in strain: Isolate HKU-39849, Isolate FRA and Isolate Frankfurt 1.
	Natural variant	73	1	N → T in strain: Isolate TC3.
	Natural variant	119	1	L → P in strain: Isolate Shanghai QXC1.
	Natural variant	154	1	S → P in strain: Isolate Urbani.

Sequences

	Sequence		Length	Mass (Da)
	P59596 [UniParc]. Last modified April 23, 2003. Version 1. Checksum: 275C37085A0215A8	FASTA	221	25,061
	10 20 30 40 50 60 MADNGTITVE ELKQLLEQWN LVIGFLFLAW IMLLQFAYSN RNRFLYIIKL VFLWLLWPVT 70 80 90 100 110 120 LACFVLAAVY RINWVTGGIA IAMACIVGLM WLSYFVASFR LFARTRSMWS FNPETNILLN 130 140 150 160 170 180 VPLRGTIVTR PLMESELVIG AVIIRGHLRM AGHSLGRCDI KDLPKEITVA TSRTLSYYKL 190 200 210 220 GASQRVGTDS GFAAYNRYRI GNYKLNTDHA GSNDNIALLV Q

References

[1]	"Characterization of a novel coronavirus associated with severe acute respiratory syndrome." Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S., Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D. , Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J., Bellini W.J. Science 300:1394-1399(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Urbani.
[2]	"The genome sequence of the SARS-associated coronavirus." Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H. , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L. Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Tor2.
[3]	"Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome." Tsui S.K.W., Chim S.S.C., Lo Y.M.D. N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
[4]	"Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China." Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M. Science 302:276-278(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate GZ50 and Isolate HKU-36871.
[5]	"The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)." Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C. Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HKU-39849.
[6]	"Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection." Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T. Lancet 361:1779-1785(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748 and Isolate sin2774.
[7]	Erratum Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T. Lancet 361:1832-1832(2003)
[8]	Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F. , Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W., Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W., Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L., Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J., Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
[9]	"The complete genome of SARS coronavirus clone TW1." Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TW1.
[10]	"SARS virus is a close relative of type II coronaviruses." Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate FRA.
[11]	"Mechanisms and enzymes involved in SARS coronavirus genome expression." Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S., Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E., Ziebuhr J. J. Gen. Virol. 84:2305-2315(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Frankfurt 1.
[12]	"Analysis of SARS coronavirus genome in Shanghai isolates." Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Shanghai QXC1.
[13]	"SARS coronavirus ZJ01 isolate membrane glycoprotein." Cong L.-M., Ding G.-Q., Lu Y.-Y., Yan J.-Q., Weng J.-Q., Cheng S.-Y., Zhang Y.-J., Mei L.-L., Wang Z.-G., Hu N., Wo J., Yao J., Zhu H.-P., Lu Q.-Y., Li M.-H., Gong L.-M., Shi W. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Isolate ZJ01.
[14]	"Genomic sequence of SARS isolate from the first fatal case in Taiwan." Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TWC.
[15]	Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
[16]	Shu H.Y., Wu K.M., Tsai S.F. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
[17]	Canducci F., Clementi M., Poli G., Vicenzi E. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HSR 1.
[18]	Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C., Yao C.-W., Chiueh T.-S., Lu J.-J., Chen A., Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate TWC2 and Isolate TWC3.
[19]	Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate AS.
[20]	"A novel severe acute respiratory syndrome coronavirus protein, U274, is transported to the cell surface and undergoes endocytosis." Tan Y.-J., Teng E., Shen S., Tan T.H.P., Goh P.-Y., Fielding B.C., Ooi E.-E., Tan H.-C., Lim S.G., Hong W. J. Virol. 78:6723-6734(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACCESSORY PROTEIN 3A.
[21]	"Subcellular localization and membrane association of SARS-CoV 3a protein." Yuan X., Li J., Shan Y., Yang Z., Zhao Z., Chen B., Yao Z., Dong B., Wang S., Chen J., Cong Y. Virus Res. 109:191-202(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACCESSORY PROTEIN 3A.
[22]	"Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT." Fielding B.C., Gunalan V., Tan T.H.P., Chou C.-F., Shen S., Khan S., Lim S.G., Hong W., Tan Y.-J. Biochem. Biophys. Res. Commun. 343:1201-1208(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACCESSORY PROTEIN 7A.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY278741 Genomic RNA. Translation: AAP13444.1.
AY274119 Genomic RNA. Translation: AAP41041.1.
AY278554 Genomic RNA. Translation: AAP13571.1.
AY282752 Genomic RNA. No translation available.
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY283798 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30034.1.
AY278489 Genomic RNA. Translation: AAP51231.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37021.1.
AY310120 Genomic RNA. Translation: AAP50489.1.
AY291315 Genomic RNA. Translation: AAP33701.1.
AY463059 Genomic RNA. Translation: AAR86790.1.
AY323974 mRNA. Translation: AAP73415.1.
AY321118 Genomic RNA. No translation available.
AY338174 Genomic RNA. Translation: AAQ01601.1.
AY338175 Genomic RNA. Translation: AAQ01613.1.
AY348314 Genomic RNA. Translation: AAP97886.1.
AP006557 Genomic RNA. Translation: BAC81352.1.
AP006558 Genomic RNA. Translation: BAC81366.1.
AP006559 Genomic RNA. Translation: BAC81380.1.
AP006560 Genomic RNA. Translation: BAC81394.1.
AP006561 Genomic RNA. Translation: BAC81408.1.
AY323977 Genomic RNA. Translation: AAP72978.1.
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94064.1.

RefSeq

NP_828855.1. NC_004718.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I6G	X-ray	2.20	C/F	88-96	[»]

ModBase

Search...

Proteomic databases

PRIDE

P59596.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1489672.

Family and domain databases

InterPro

IPR002574. Corona_M.
[Graphical view]

Pfam

PF01635. Corona_M. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P59596.

Entry information

Entry name

VME1_CVHSA

Accession

Primary (citable) accession number: P59596
Secondary accession number(s): Q6S8E5 , Q7T6Q8, Q7T6R1, Q7T6R5, Q7T6S0, Q7T6S3, Q7T726, Q7T7P6, Q7T7S6, Q7TA12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program