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Protein

Nucleoprotein

Gene

N

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.By similarity1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:9a
OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Taxonomic identifieri227859 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]
Proteomesi
  • UP000000354 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001060031 – 422NucleoproteinAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177Phosphoserine; by host1

Post-translational modificationi

Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP59595.

Interactioni

Subunit structurei

Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the membrane protein M (PubMed:15351485). Interacts (when phosphorylated) with host DDX1; this interaction is essential to produce a full set of subgenomic and genomic RNAs. Interacts with envelope small membrane protein E (PubMed:24766657). Interacts with host HNRNPA1; this interaction may be involved in the regulation of viral RNA synthesis (PubMed:15862300).By similarity3 Publications

Protein-protein interaction databases

MINTiMINT-1346173.

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 59Combined sources4
Beta strandi61 – 63Combined sources3
Beta strandi77 – 79Combined sources3
Helixi81 – 83Combined sources3
Beta strandi85 – 91Combined sources7
Beta strandi94 – 96Combined sources3
Turni98 – 100Combined sources3
Beta strandi102 – 104Combined sources3
Beta strandi108 – 113Combined sources6
Turni118 – 121Combined sources4
Beta strandi131 – 135Combined sources5
Beta strandi140 – 142Combined sources3
Turni145 – 147Combined sources3
Turni152 – 154Combined sources3
Beta strandi254 – 258Combined sources5
Helixi260 – 262Combined sources3
Helixi271 – 275Combined sources5
Turni282 – 284Combined sources3
Helixi290 – 295Combined sources6
Helixi296 – 298Combined sources3
Helixi302 – 306Combined sources5
Helixi312 – 318Combined sources7
Beta strandi319 – 325Combined sources7
Beta strandi330 – 340Combined sources11
Helixi347 – 357Combined sources11
Helixi360 – 363Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SSKNMR-A45-181[»]
1X7QX-ray1.45C362-370[»]
2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
2GIBX-ray1.75A/B270-370[»]
2JW8NMR-A/B248-365[»]
2OFZX-ray1.17A49-174[»]
2OG3X-ray1.85A49-129[»]
3I6LX-ray2.40F346-354[»]
ProteinModelPortaliP59595.
SMRiP59595.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59595.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 181RNA-bindingAdd BLAST137
Regioni259 – 367DimerizationBy similarityAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 213Ser-richAdd BLAST33
Compositional biasi220 – 225Poly-Leu6
Compositional biasi240 – 245Poly-Gln6
Compositional biasi370 – 376Poly-Lys7

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P59595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNGPQSNQ RSAPRITFGG PTDSTDNNQN GGRNGARPKQ RRPQGLPNNT
60 70 80 90 100
ASWFTALTQH GKEELRFPRG QGVPINTNSG PDDQIGYYRR ATRRVRGGDG
110 120 130 140 150
KMKELSPRWY FYYLGTGPEA SLPYGANKEG IVWVATEGAL NTPKDHIGTR
160 170 180 190 200
NPNNNAATVL QLPQGTTLPK GFYAEGSRGG SQASSRSSSR SRGNSRNSTP
210 220 230 240 250
GSSRGNSPAR MASGGGETAL ALLLLDRLNQ LESKVSGKGQ QQQGQTVTKK
260 270 280 290 300
SAAEASKKPR QKRTATKQYN VTQAFGRRGP EQTQGNFGDQ DLIRQGTDYK
310 320 330 340 350
HWPQIAQFAP SASAFFGMSR IGMEVTPSGT WLTYHGAIKL DDKDPQFKDN
360 370 380 390 400
VILLNKHIDA YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ PTVTLLPAAD
410 420
MDDFSRQLQN SMSGASADST QA
Length:422
Mass (Da):46,025
Last modified:April 23, 2003 - v1
Checksum:i43FC8750F1253034
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti50T → I in strain: Isolate Frankfurt 1 and Isolate FRA. 1
Natural varianti154N → Y in strain: Isolate BJ03. 1
Natural varianti193G → C in strain: Isolate CUHK-Su10. 1
Natural varianti325 – 326VT → AA in strain: Isolate Shanghai LY. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA. Translation: AAP13445.1.
AY274119 Genomic RNA. Translation: AAP41047.1.
AY278554 Genomic RNA. Translation: AAP13814.1.
AY282752 Genomic RNA. Translation: AAP30714.1.
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30037.1.
AY278489 Genomic RNA. Translation: AAP51234.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37024.1.
AY310120 Genomic RNA. Translation: AAP50495.1.
AY291315 Genomic RNA. Translation: AAP33707.1.
AY307165 Genomic RNA. Translation: AAP49024.1.
AY290752 Genomic RNA. Translation: AAP44772.1.
AY321118 Genomic RNA. No translation available.
AY322208 Genomic RNA. Translation: AAP82974.1.
AY338174 Genomic RNA. Translation: AAQ01605.1.
AY338175 Genomic RNA. Translation: AAQ01617.1.
AY348314 Genomic RNA. Translation: AAP97890.1.
AP006557 Genomic RNA. Translation: BAC81358.1.
AP006558 Genomic RNA. Translation: BAC81372.1.
AP006559 Genomic RNA. Translation: BAC81386.1.
AP006560 Genomic RNA. Translation: BAC81400.1.
AP006561 Genomic RNA. Translation: BAC81414.1.
AY323977 Genomic RNA. Translation: AAP72984.1.
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94070.1.
RefSeqiNP_828858.1. NC_004718.3.

Genome annotation databases

GeneIDi1489678.
KEGGivg:1489678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA. Translation: AAP13445.1.
AY274119 Genomic RNA. Translation: AAP41047.1.
AY278554 Genomic RNA. Translation: AAP13814.1.
AY282752 Genomic RNA. Translation: AAP30714.1.
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30037.1.
AY278489 Genomic RNA. Translation: AAP51234.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37024.1.
AY310120 Genomic RNA. Translation: AAP50495.1.
AY291315 Genomic RNA. Translation: AAP33707.1.
AY307165 Genomic RNA. Translation: AAP49024.1.
AY290752 Genomic RNA. Translation: AAP44772.1.
AY321118 Genomic RNA. No translation available.
AY322208 Genomic RNA. Translation: AAP82974.1.
AY338174 Genomic RNA. Translation: AAQ01605.1.
AY338175 Genomic RNA. Translation: AAQ01617.1.
AY348314 Genomic RNA. Translation: AAP97890.1.
AP006557 Genomic RNA. Translation: BAC81358.1.
AP006558 Genomic RNA. Translation: BAC81372.1.
AP006559 Genomic RNA. Translation: BAC81386.1.
AP006560 Genomic RNA. Translation: BAC81400.1.
AP006561 Genomic RNA. Translation: BAC81414.1.
AY323977 Genomic RNA. Translation: AAP72984.1.
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94070.1.
RefSeqiNP_828858.1. NC_004718.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SSKNMR-A45-181[»]
1X7QX-ray1.45C362-370[»]
2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
2GIBX-ray1.75A/B270-370[»]
2JW8NMR-A/B248-365[»]
2OFZX-ray1.17A49-174[»]
2OG3X-ray1.85A49-129[»]
3I6LX-ray2.40F346-354[»]
ProteinModelPortaliP59595.
SMRiP59595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1346173.

Proteomic databases

PRIDEiP59595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489678.
KEGGivg:1489678.

Miscellaneous databases

EvolutionaryTraceiP59595.

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_CVHSA
AccessioniPrimary (citable) accession number: P59595
Secondary accession number(s): Q7T3Z4
, Q7TA14, Q7TF99, Q80E50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.