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P59595 (NCAP_CVHSA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name=NC
Short name=Protein N
Gene names
Name:N
ORF Names:9a
OrganismHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus) [Reference proteome]
Taxonomic identifier227859 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid By similarity.

Subunit structure

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein By similarity.

Subcellular location

Virion. Host cytoplasmhost perinuclear region. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus. Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. Not found in the nucleolus. Ref.22

Sequence similarities

Belongs to the coronavirus group 2a nucleocapsid protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Nucleoprotein
PRO_0000106003

Regions

Region45 – 181137RNA-binding
Region259 – 367109Dimerization By similarity
Compositional bias181 – 21333Ser-rich
Compositional bias220 – 2256Poly-Leu
Compositional bias240 – 2456Poly-Gln
Compositional bias370 – 3767Poly-Lys

Natural variations

Natural variant501T → I in strain: Isolate Frankfurt 1 and Isolate FRA.
Natural variant1541N → Y in strain: Isolate BJ03.
Natural variant1931G → C in strain: Isolate CUHK-Su10.
Natural variant325 – 3262VT → AA in strain: Isolate Shanghai LY.

Secondary structure

................................................... 422
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P59595 [UniParc].

Last modified April 23, 2003. Version 1.
Checksum: 43FC8750F1253034

FASTA42246,025
        10         20         30         40         50         60 
MSDNGPQSNQ RSAPRITFGG PTDSTDNNQN GGRNGARPKQ RRPQGLPNNT ASWFTALTQH 

        70         80         90        100        110        120 
GKEELRFPRG QGVPINTNSG PDDQIGYYRR ATRRVRGGDG KMKELSPRWY FYYLGTGPEA 

       130        140        150        160        170        180 
SLPYGANKEG IVWVATEGAL NTPKDHIGTR NPNNNAATVL QLPQGTTLPK GFYAEGSRGG 

       190        200        210        220        230        240 
SQASSRSSSR SRGNSRNSTP GSSRGNSPAR MASGGGETAL ALLLLDRLNQ LESKVSGKGQ 

       250        260        270        280        290        300 
QQQGQTVTKK SAAEASKKPR QKRTATKQYN VTQAFGRRGP EQTQGNFGDQ DLIRQGTDYK 

       310        320        330        340        350        360 
HWPQIAQFAP SASAFFGMSR IGMEVTPSGT WLTYHGAIKL DDKDPQFKDN VILLNKHIDA 

       370        380        390        400        410        420 
YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ PTVTLLPAAD MDDFSRQLQN SMSGASADST 


QA 

« Hide

References

[1]"Characterization of a novel coronavirus associated with severe acute respiratory syndrome."
Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.P., Penaranda S., Bankamp B., Maher K., Chen M.-H., Tong S., Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D. expand/collapse author list , Peret T.C.T., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., Guenther S., Osterhaus A.D.M.E., Drosten C., Pallansch M.A., Anderson L.J., Bellini W.J.
Science 300:1394-1399(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Urbani.
[2]"The genome sequence of the SARS-associated coronavirus."
Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H. expand/collapse author list , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Tor2.
[3]"Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
[4]"Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China."
Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.
Science 302:276-278(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate GZ50 and Isolate HKU-36871.
[5]"The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HKU-39849.
[6]"Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection."
Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.
Lancet 361:1779-1785(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748 and Isolate sin2774.
[7]Erratum
Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.
Lancet 361:1832-1832(2003)
[8]Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lu F. expand/collapse author list , Tan G., Yang R., Cao W.S., Wang J., Chen W., Cong L., Deng Y., Dong W., Han Y., Hu W., Lei M., Li C., Li G., Li G., Li H., Li S., Li S., Li W., Li W., Lin W., Liu J., Liu Z., Lu H., Ni P., Qi Q., Sun Y., Tang L., Tong Z., Wang J., Wang X., Wu Q., Xi Y., Xu Z., Yang L., Ye C., Ye J., Zhang B., Zhang F., Zhang J., Zhang X., Zhou J., Yang H.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
[9]"The complete genome of SARS coronavirus clone TW1."
Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TW1.
[10]"SARS virus is a close relative of type II coronaviruses."
Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate FRA.
[11]"Mechanisms and enzymes involved in SARS coronavirus genome expression."
Thiel V., Ivanov K.A., Putics A., Hertzig T., Schelle B., Bayer S., Weissbrich B., Snijder E.J., Rabenau H., Doerr H.W., Gorbalenya A.E., Ziebuhr J.
J. Gen. Virol. 84:2305-2315(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Frankfurt 1.
[12]Sun K., Anwar A., Gupta V., Tabiin M.T., Atkinson R., Chandrasekarn A., August T.J.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[13]"Severe acute respiratory syndrome-associated coronavirus genotype and its characterization."
Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y., Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q. expand/collapse author list , Yao P., Bo X., Wo J., Wang S., Hu S.
Chin. Med. J. 116:1288-1292(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate ZJ01.
[14]"Genomic sequence of SARS isolate from the first fatal case in Taiwan."
Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TWC.
[15]Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Shanghai LY.
[16]Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
[17]"The complete genome of SARS coronavirus TWH."
Shu H.Y., Wu K.M., Tsai S.F.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
[18]Canducci F., Clementi M., Poli G., Vicenzi E.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HSR 1.
[19]Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate TWC2 and Isolate TWC3.
[20]Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate AS.
[21]"Ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the N-terminal domain of N protein."
Saikatendu K.S., Joseph J.S., Subramanian V., Neuman B.W., Buchmeier M.J., Stevens R.C., Kuhn P.
J. Virol. 81:3913-3921(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.
Strain: Isolate Tor2.
[22]"The intracellular sites of early replication and budding of SARS-coronavirus."
Stertz S., Reichelt M., Spiegel M., Kuri T., Martinez-Sobrido L., Garcia-Sastre A., Weber F., Kochs G.
Virology 361:304-315(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY278741 Genomic RNA. Translation: AAP13445.1.
AY274119 Genomic RNA. Translation: AAP41047.1.
AY278554 Genomic RNA. Translation: AAP13814.1.
AY282752 Genomic RNA. Translation: AAP30714.1.
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30037.1.
AY278489 Genomic RNA. Translation: AAP51234.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37024.1.
AY310120 Genomic RNA. Translation: AAP50495.1.
AY291315 Genomic RNA. Translation: AAP33707.1.
AY307165 Genomic RNA. Translation: AAP49024.1.
AY290752 Genomic RNA. Translation: AAP44772.1.
AY321118 Genomic RNA. No translation available.
AY322208 Genomic RNA. Translation: AAP82974.1.
AY338174 Genomic RNA. Translation: AAQ01605.1.
AY338175 Genomic RNA. Translation: AAQ01617.1.
AY348314 Genomic RNA. Translation: AAP97890.1.
AP006557 Genomic RNA. Translation: BAC81358.1.
AP006558 Genomic RNA. Translation: BAC81372.1.
AP006559 Genomic RNA. Translation: BAC81386.1.
AP006560 Genomic RNA. Translation: BAC81400.1.
AP006561 Genomic RNA. Translation: BAC81414.1.
AY323977 Genomic RNA. Translation: AAP72984.1.
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94070.1.
RefSeqNP_828858.1. NC_004718.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SSKNMR-A45-181[»]
1X7QX-ray1.45C362-370[»]
2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
2GIBX-ray1.75A/B270-370[»]
2JW8NMR-A/B248-365[»]
2OFZX-ray1.17A49-174[»]
2OG3X-ray1.85A49-174[»]
3I6LX-ray2.40F346-354[»]
ProteinModelPortalP59595.
SMRP59595. Positions 45-181, 251-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1346173.

Proteomic databases

PRIDEP59595.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1489678.

Family and domain databases

InterProIPR001218. Corona_nucleocap.
[Graphical view]
PfamPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFPIRSF003888. Corona_nucleocap. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP59595.

Entry information

Entry nameNCAP_CVHSA
AccessionPrimary (citable) accession number: P59595
Secondary accession number(s): Q7T3Z4 expand/collapse secondary AC list , Q7TA14, Q7TF99, Q80E50
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: March 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references