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P59595

- NCAP_CVHSA

UniProt

P59595 - NCAP_CVHSA

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Protein
Nucleoprotein
Gene
N, 9a
Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
NC
Short name:
Protein N
Gene namesi
Name:N
ORF Names:9a
OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Taxonomic identifieri227859 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]
ProteomesiUP000000354: Genome

Subcellular locationi

Virion. Host cytoplasmhost perinuclear region. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus
Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. Not found in the nucleolus.1 Publication

GO - Cellular componenti

  1. host cell Golgi apparatus Source: UniProtKB-SubCell
  2. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. ribonucleoprotein complex Source: UniProtKB-KW
  5. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Nucleoprotein
PRO_0000106003Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP59595.

Interactioni

Subunit structurei

Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein By similarity.

Protein-protein interaction databases

MINTiMINT-1346173.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 594
Beta strandi61 – 633
Beta strandi77 – 793
Helixi81 – 833
Beta strandi85 – 917
Beta strandi94 – 963
Turni98 – 1003
Beta strandi102 – 1043
Beta strandi108 – 1136
Turni118 – 1214
Beta strandi131 – 1355
Beta strandi140 – 1423
Turni145 – 1473
Turni152 – 1543
Beta strandi254 – 2585
Helixi260 – 2623
Helixi271 – 2755
Turni282 – 2843
Helixi290 – 2956
Helixi296 – 2983
Helixi302 – 3065
Helixi312 – 3187
Beta strandi319 – 3257
Beta strandi330 – 34011
Helixi347 – 35711
Helixi360 – 3634

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SSKNMR-A45-181[»]
1X7QX-ray1.45C362-370[»]
2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
2GIBX-ray1.75A/B270-370[»]
2JW8NMR-A/B248-365[»]
2OFZX-ray1.17A49-174[»]
2OG3X-ray1.85A49-129[»]
3I6LX-ray2.40F346-354[»]
ProteinModelPortaliP59595.
SMRiP59595. Positions 45-181, 251-365.

Miscellaneous databases

EvolutionaryTraceiP59595.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 181137RNA-binding
Add
BLAST
Regioni259 – 367109Dimerization By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi181 – 21333Ser-rich
Add
BLAST
Compositional biasi220 – 2256Poly-Leu
Compositional biasi240 – 2456Poly-Gln
Compositional biasi370 – 3767Poly-Lys

Sequence similaritiesi

Family and domain databases

InterProiIPR001218. Corona_nucleocap.
[Graphical view]
PfamiPF00937. Corona_nucleoca. 1 hit.
[Graphical view]
PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

Sequencei

Sequence statusi: Complete.

P59595-1 [UniParc]FASTAAdd to Basket

« Hide

MSDNGPQSNQ RSAPRITFGG PTDSTDNNQN GGRNGARPKQ RRPQGLPNNT    50
ASWFTALTQH GKEELRFPRG QGVPINTNSG PDDQIGYYRR ATRRVRGGDG 100
KMKELSPRWY FYYLGTGPEA SLPYGANKEG IVWVATEGAL NTPKDHIGTR 150
NPNNNAATVL QLPQGTTLPK GFYAEGSRGG SQASSRSSSR SRGNSRNSTP 200
GSSRGNSPAR MASGGGETAL ALLLLDRLNQ LESKVSGKGQ QQQGQTVTKK 250
SAAEASKKPR QKRTATKQYN VTQAFGRRGP EQTQGNFGDQ DLIRQGTDYK 300
HWPQIAQFAP SASAFFGMSR IGMEVTPSGT WLTYHGAIKL DDKDPQFKDN 350
VILLNKHIDA YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ PTVTLLPAAD 400
MDDFSRQLQN SMSGASADST QA 422
Length:422
Mass (Da):46,025
Last modified:April 23, 2003 - v1
Checksum:i43FC8750F1253034
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501T → I in strain: Isolate Frankfurt 1 and Isolate FRA.
Natural varianti154 – 1541N → Y in strain: Isolate BJ03.
Natural varianti193 – 1931G → C in strain: Isolate CUHK-Su10.
Natural varianti325 – 3262VT → AA in strain: Isolate Shanghai LY.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY278741 Genomic RNA. Translation: AAP13445.1.
AY274119 Genomic RNA. Translation: AAP41047.1.
AY278554 Genomic RNA. Translation: AAP13814.1.
AY282752 Genomic RNA. Translation: AAP30714.1.
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30037.1.
AY278489 Genomic RNA. Translation: AAP51234.1.
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37024.1.
AY310120 Genomic RNA. Translation: AAP50495.1.
AY291315 Genomic RNA. Translation: AAP33707.1.
AY307165 Genomic RNA. Translation: AAP49024.1.
AY290752 Genomic RNA. Translation: AAP44772.1.
AY321118 Genomic RNA. No translation available.
AY322208 Genomic RNA. Translation: AAP82974.1.
AY338174 Genomic RNA. Translation: AAQ01605.1.
AY338175 Genomic RNA. Translation: AAQ01617.1.
AY348314 Genomic RNA. Translation: AAP97890.1.
AP006557 Genomic RNA. Translation: BAC81358.1.
AP006558 Genomic RNA. Translation: BAC81372.1.
AP006559 Genomic RNA. Translation: BAC81386.1.
AP006560 Genomic RNA. Translation: BAC81400.1.
AP006561 Genomic RNA. Translation: BAC81414.1.
AY323977 Genomic RNA. Translation: AAP72984.1.
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94070.1.
RefSeqiNP_828858.1. NC_004718.3.

Genome annotation databases

GeneIDi1489678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY278741 Genomic RNA. Translation: AAP13445.1 .
AY274119 Genomic RNA. Translation: AAP41047.1 .
AY278554 Genomic RNA. Translation: AAP13814.1 .
AY282752 Genomic RNA. Translation: AAP30714.1 .
AY304492 Genomic RNA. No translation available.
AY304495 Genomic RNA. No translation available.
AY278491 Genomic RNA. No translation available.
AY283794 Genomic RNA. No translation available.
AY283795 Genomic RNA. No translation available.
AY283796 Genomic RNA. No translation available.
AY283797 Genomic RNA. No translation available.
AY278487 Genomic RNA. No translation available.
AY278488 Genomic RNA. Translation: AAP30037.1 .
AY278489 Genomic RNA. Translation: AAP51234.1 .
AY278490 Genomic RNA. No translation available.
AY279354 Genomic RNA. No translation available.
AY291451 Genomic RNA. Translation: AAP37024.1 .
AY310120 Genomic RNA. Translation: AAP50495.1 .
AY291315 Genomic RNA. Translation: AAP33707.1 .
AY307165 Genomic RNA. Translation: AAP49024.1 .
AY290752 Genomic RNA. Translation: AAP44772.1 .
AY321118 Genomic RNA. No translation available.
AY322208 Genomic RNA. Translation: AAP82974.1 .
AY338174 Genomic RNA. Translation: AAQ01605.1 .
AY338175 Genomic RNA. Translation: AAQ01617.1 .
AY348314 Genomic RNA. Translation: AAP97890.1 .
AP006557 Genomic RNA. Translation: BAC81358.1 .
AP006558 Genomic RNA. Translation: BAC81372.1 .
AP006559 Genomic RNA. Translation: BAC81386.1 .
AP006560 Genomic RNA. Translation: BAC81400.1 .
AP006561 Genomic RNA. Translation: BAC81414.1 .
AY323977 Genomic RNA. Translation: AAP72984.1 .
AY362698 Genomic RNA. No translation available.
AY362699 Genomic RNA. No translation available.
AY427439 Genomic RNA. Translation: AAQ94070.1 .
RefSeqi NP_828858.1. NC_004718.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SSK NMR - A 45-181 [» ]
1X7Q X-ray 1.45 C 362-370 [» ]
2CJR X-ray 2.50 A/B/C/D/E/F/G/H 248-365 [» ]
2GIB X-ray 1.75 A/B 270-370 [» ]
2JW8 NMR - A/B 248-365 [» ]
2OFZ X-ray 1.17 A 49-174 [» ]
2OG3 X-ray 1.85 A 49-129 [» ]
3I6L X-ray 2.40 F 346-354 [» ]
ProteinModelPortali P59595.
SMRi P59595. Positions 45-181, 251-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1346173.

Proteomic databases

PRIDEi P59595.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1489678.

Miscellaneous databases

EvolutionaryTracei P59595.

Family and domain databases

InterProi IPR001218. Corona_nucleocap.
[Graphical view ]
Pfami PF00937. Corona_nucleoca. 1 hit.
[Graphical view ]
PIRSFi PIRSF003888. Corona_nucleocap. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Urbani.
  2. "The genome sequence of the SARS-associated coronavirus."
    Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H.
    , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
    Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Tor2.
  3. "Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
    Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
    N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
  4. "Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China."
    Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.
    Science 302:276-278(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate GZ50 and Isolate HKU-36871.
  5. "The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
    Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
    Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HKU-39849.
  6. "Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection."
    Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.
    Lancet 361:1779-1785(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748 and Isolate sin2774.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
  8. "The complete genome of SARS coronavirus clone TW1."
    Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TW1.
  9. "SARS virus is a close relative of type II coronaviruses."
    Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate FRA.
  10. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Frankfurt 1.
  11. Sun K., Anwar A., Gupta V., Tabiin M.T., Atkinson R., Chandrasekarn A., August T.J.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  12. "Severe acute respiratory syndrome-associated coronavirus genotype and its characterization."
    Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y., Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.
    , Yao P., Bo X., Wo J., Wang S., Hu S.
    Chin. Med. J. 116:1288-1292(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate ZJ01.
  13. "Genomic sequence of SARS isolate from the first fatal case in Taiwan."
    Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TWC.
  14. Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Shanghai LY.
  15. Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
  16. "The complete genome of SARS coronavirus TWH."
    Shu H.Y., Wu K.M., Tsai S.F.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
  17. Canducci F., Clementi M., Poli G., Vicenzi E.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HSR 1.
  18. Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate TWC2 and Isolate TWC3.
  19. Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate AS.
  20. "Ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the N-terminal domain of N protein."
    Saikatendu K.S., Joseph J.S., Subramanian V., Neuman B.W., Buchmeier M.J., Stevens R.C., Kuhn P.
    J. Virol. 81:3913-3921(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
    Strain: Isolate Tor2.
  21. "The intracellular sites of early replication and budding of SARS-coronavirus."
    Stertz S., Reichelt M., Spiegel M., Kuri T., Martinez-Sobrido L., Garcia-Sastre A., Weber F., Kochs G.
    Virology 361:304-315(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNCAP_CVHSA
AccessioniPrimary (citable) accession number: P59595
Secondary accession number(s): Q7T3Z4
, Q7TA14, Q7TF99, Q80E50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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