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P59595

- NCAP_CVHSA

UniProt

P59595 - NCAP_CVHSA

Protein

Nucleoprotein

Gene

N

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    NC
    Short name:
    Protein N
    Gene namesi
    Name:N
    ORF Names:9a
    OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
    Taxonomic identifieri227859 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Paguma larvata (Masked palm civet) [TaxID: 9675]
    ProteomesiUP000000354: Genome

    Subcellular locationi

    Virion 1 Publication. Host cytoplasmhost perinuclear region 1 Publication. Host endoplasmic reticulum-Golgi intermediate compartment 1 Publication. Host Golgi apparatus 1 Publication
    Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. Not found in the nucleolus.

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell Golgi apparatus Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. ribonucleoprotein complex Source: UniProtKB-KW
    5. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host Golgi apparatus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 422422NucleoproteinPRO_0000106003Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP59595.

    Interactioni

    Subunit structurei

    Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-1346173.

    Structurei

    Secondary structure

    1
    422
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi56 – 594
    Beta strandi61 – 633
    Beta strandi77 – 793
    Helixi81 – 833
    Beta strandi85 – 917
    Beta strandi94 – 963
    Turni98 – 1003
    Beta strandi102 – 1043
    Beta strandi108 – 1136
    Turni118 – 1214
    Beta strandi131 – 1355
    Beta strandi140 – 1423
    Turni145 – 1473
    Turni152 – 1543
    Beta strandi254 – 2585
    Helixi260 – 2623
    Helixi271 – 2755
    Turni282 – 2843
    Helixi290 – 2956
    Helixi296 – 2983
    Helixi302 – 3065
    Helixi312 – 3187
    Beta strandi319 – 3257
    Beta strandi330 – 34011
    Helixi347 – 35711
    Helixi360 – 3634

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SSKNMR-A45-181[»]
    1X7QX-ray1.45C362-370[»]
    2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
    2GIBX-ray1.75A/B270-370[»]
    2JW8NMR-A/B248-365[»]
    2OFZX-ray1.17A49-174[»]
    2OG3X-ray1.85A49-129[»]
    3I6LX-ray2.40F346-354[»]
    ProteinModelPortaliP59595.
    SMRiP59595. Positions 45-181, 251-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP59595.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni45 – 181137RNA-bindingAdd
    BLAST
    Regioni259 – 367109DimerizationBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi181 – 21333Ser-richAdd
    BLAST
    Compositional biasi220 – 2256Poly-Leu
    Compositional biasi240 – 2456Poly-Gln
    Compositional biasi370 – 3767Poly-Lys

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR001218. Corona_nucleocap.
    [Graphical view]
    PfamiPF00937. Corona_nucleoca. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003888. Corona_nucleocap. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P59595-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNGPQSNQ RSAPRITFGG PTDSTDNNQN GGRNGARPKQ RRPQGLPNNT    50
    ASWFTALTQH GKEELRFPRG QGVPINTNSG PDDQIGYYRR ATRRVRGGDG 100
    KMKELSPRWY FYYLGTGPEA SLPYGANKEG IVWVATEGAL NTPKDHIGTR 150
    NPNNNAATVL QLPQGTTLPK GFYAEGSRGG SQASSRSSSR SRGNSRNSTP 200
    GSSRGNSPAR MASGGGETAL ALLLLDRLNQ LESKVSGKGQ QQQGQTVTKK 250
    SAAEASKKPR QKRTATKQYN VTQAFGRRGP EQTQGNFGDQ DLIRQGTDYK 300
    HWPQIAQFAP SASAFFGMSR IGMEVTPSGT WLTYHGAIKL DDKDPQFKDN 350
    VILLNKHIDA YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ PTVTLLPAAD 400
    MDDFSRQLQN SMSGASADST QA 422
    Length:422
    Mass (Da):46,025
    Last modified:April 23, 2003 - v1
    Checksum:i43FC8750F1253034
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti50 – 501T → I in strain: Isolate Frankfurt 1 and Isolate FRA.
    Natural varianti154 – 1541N → Y in strain: Isolate BJ03.
    Natural varianti193 – 1931G → C in strain: Isolate CUHK-Su10.
    Natural varianti325 – 3262VT → AA in strain: Isolate Shanghai LY.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY278741 Genomic RNA. Translation: AAP13445.1.
    AY274119 Genomic RNA. Translation: AAP41047.1.
    AY278554 Genomic RNA. Translation: AAP13814.1.
    AY282752 Genomic RNA. Translation: AAP30714.1.
    AY304492 Genomic RNA. No translation available.
    AY304495 Genomic RNA. No translation available.
    AY278491 Genomic RNA. No translation available.
    AY283794 Genomic RNA. No translation available.
    AY283795 Genomic RNA. No translation available.
    AY283796 Genomic RNA. No translation available.
    AY283797 Genomic RNA. No translation available.
    AY278487 Genomic RNA. No translation available.
    AY278488 Genomic RNA. Translation: AAP30037.1.
    AY278489 Genomic RNA. Translation: AAP51234.1.
    AY278490 Genomic RNA. No translation available.
    AY279354 Genomic RNA. No translation available.
    AY291451 Genomic RNA. Translation: AAP37024.1.
    AY310120 Genomic RNA. Translation: AAP50495.1.
    AY291315 Genomic RNA. Translation: AAP33707.1.
    AY307165 Genomic RNA. Translation: AAP49024.1.
    AY290752 Genomic RNA. Translation: AAP44772.1.
    AY321118 Genomic RNA. No translation available.
    AY322208 Genomic RNA. Translation: AAP82974.1.
    AY338174 Genomic RNA. Translation: AAQ01605.1.
    AY338175 Genomic RNA. Translation: AAQ01617.1.
    AY348314 Genomic RNA. Translation: AAP97890.1.
    AP006557 Genomic RNA. Translation: BAC81358.1.
    AP006558 Genomic RNA. Translation: BAC81372.1.
    AP006559 Genomic RNA. Translation: BAC81386.1.
    AP006560 Genomic RNA. Translation: BAC81400.1.
    AP006561 Genomic RNA. Translation: BAC81414.1.
    AY323977 Genomic RNA. Translation: AAP72984.1.
    AY362698 Genomic RNA. No translation available.
    AY362699 Genomic RNA. No translation available.
    AY427439 Genomic RNA. Translation: AAQ94070.1.
    RefSeqiNP_828858.1. NC_004718.3.

    Genome annotation databases

    GeneIDi1489678.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY278741 Genomic RNA. Translation: AAP13445.1 .
    AY274119 Genomic RNA. Translation: AAP41047.1 .
    AY278554 Genomic RNA. Translation: AAP13814.1 .
    AY282752 Genomic RNA. Translation: AAP30714.1 .
    AY304492 Genomic RNA. No translation available.
    AY304495 Genomic RNA. No translation available.
    AY278491 Genomic RNA. No translation available.
    AY283794 Genomic RNA. No translation available.
    AY283795 Genomic RNA. No translation available.
    AY283796 Genomic RNA. No translation available.
    AY283797 Genomic RNA. No translation available.
    AY278487 Genomic RNA. No translation available.
    AY278488 Genomic RNA. Translation: AAP30037.1 .
    AY278489 Genomic RNA. Translation: AAP51234.1 .
    AY278490 Genomic RNA. No translation available.
    AY279354 Genomic RNA. No translation available.
    AY291451 Genomic RNA. Translation: AAP37024.1 .
    AY310120 Genomic RNA. Translation: AAP50495.1 .
    AY291315 Genomic RNA. Translation: AAP33707.1 .
    AY307165 Genomic RNA. Translation: AAP49024.1 .
    AY290752 Genomic RNA. Translation: AAP44772.1 .
    AY321118 Genomic RNA. No translation available.
    AY322208 Genomic RNA. Translation: AAP82974.1 .
    AY338174 Genomic RNA. Translation: AAQ01605.1 .
    AY338175 Genomic RNA. Translation: AAQ01617.1 .
    AY348314 Genomic RNA. Translation: AAP97890.1 .
    AP006557 Genomic RNA. Translation: BAC81358.1 .
    AP006558 Genomic RNA. Translation: BAC81372.1 .
    AP006559 Genomic RNA. Translation: BAC81386.1 .
    AP006560 Genomic RNA. Translation: BAC81400.1 .
    AP006561 Genomic RNA. Translation: BAC81414.1 .
    AY323977 Genomic RNA. Translation: AAP72984.1 .
    AY362698 Genomic RNA. No translation available.
    AY362699 Genomic RNA. No translation available.
    AY427439 Genomic RNA. Translation: AAQ94070.1 .
    RefSeqi NP_828858.1. NC_004718.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SSK NMR - A 45-181 [» ]
    1X7Q X-ray 1.45 C 362-370 [» ]
    2CJR X-ray 2.50 A/B/C/D/E/F/G/H 248-365 [» ]
    2GIB X-ray 1.75 A/B 270-370 [» ]
    2JW8 NMR - A/B 248-365 [» ]
    2OFZ X-ray 1.17 A 49-174 [» ]
    2OG3 X-ray 1.85 A 49-129 [» ]
    3I6L X-ray 2.40 F 346-354 [» ]
    ProteinModelPortali P59595.
    SMRi P59595. Positions 45-181, 251-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1346173.

    Proteomic databases

    PRIDEi P59595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489678.

    Miscellaneous databases

    EvolutionaryTracei P59595.

    Family and domain databases

    InterProi IPR001218. Corona_nucleocap.
    [Graphical view ]
    Pfami PF00937. Corona_nucleoca. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003888. Corona_nucleocap. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Urbani.
    2. "The genome sequence of the SARS-associated coronavirus."
      Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H.
      , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
      Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Tor2.
    3. "Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
      Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
      N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
    4. "Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China."
      Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.
      Science 302:276-278(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate GZ50 and Isolate HKU-36871.
    5. "The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
      Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
      Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HKU-39849.
    6. "Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection."
      Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.
      Lancet 361:1779-1785(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748 and Isolate sin2774.
    7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
    8. "The complete genome of SARS coronavirus clone TW1."
      Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TW1.
    9. "SARS virus is a close relative of type II coronaviruses."
      Eickmann M., Becker S., Klenk H.-D., Doerr H.W., Stadler K., Censini S., Guidotti S., Masignani V., Scarselli M., Mora M., Donati C., Han J., Song H.C., Abrignani S., Covacci A., Rappuoli R.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate FRA.
    10. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Frankfurt 1.
    11. Sun K., Anwar A., Gupta V., Tabiin M.T., Atkinson R., Chandrasekarn A., August T.J.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    12. "Severe acute respiratory syndrome-associated coronavirus genotype and its characterization."
      Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y., Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.
      , Yao P., Bo X., Wo J., Wang S., Hu S.
      Chin. Med. J. 116:1288-1292(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate ZJ01.
    13. "Genomic sequence of SARS isolate from the first fatal case in Taiwan."
      Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TWC.
    14. Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Shanghai LY.
    15. Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
    16. "The complete genome of SARS coronavirus TWH."
      Shu H.Y., Wu K.M., Tsai S.F.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
    17. Canducci F., Clementi M., Poli G., Vicenzi E.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HSR 1.
    18. Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee H.-C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chen P.-J., Su I.-J.
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TWC2 and Isolate TWC3.
    19. Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate AS.
    20. "Ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the N-terminal domain of N protein."
      Saikatendu K.S., Joseph J.S., Subramanian V., Neuman B.W., Buchmeier M.J., Stevens R.C., Kuhn P.
      J. Virol. 81:3913-3921(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING.
      Strain: Isolate Tor2.
    21. "The intracellular sites of early replication and budding of SARS-coronavirus."
      Stertz S., Reichelt M., Spiegel M., Kuri T., Martinez-Sobrido L., Garcia-Sastre A., Weber F., Kochs G.
      Virology 361:304-315(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiNCAP_CVHSA
    AccessioniPrimary (citable) accession number: P59595
    Secondary accession number(s): Q7T3Z4
    , Q7TA14, Q7TF99, Q80E50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3