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Protein

Nucleoprotein

Gene

N

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding, Viral nucleoprotein
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NucleoproteinUniRule annotation
Alternative name(s):
Nucleocapsid proteinUniRule annotation
Short name:
NCUniRule annotation
Short name:
Protein NUniRule annotation
Gene namesi
Name:NUniRule annotation
ORF Names:9a
OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Taxonomic identifieri227859 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Paguma larvata (Masked palm civet) [TaxID: 9675]
Proteomesi
  • UP000160648 Componenti: Genome
  • UP000121636 Componenti: Genome
  • UP000146181 Componenti: Genome
  • UP000116947 Componenti: Genome
  • UP000145651 Componenti: Genome
  • UP000146296 Componenti: Genome
  • UP000146108 Componenti: Genome
  • UP000172416 Componenti: Genome
  • UP000137377 Componenti: Genome
  • UP000000354 Componenti: Genome
  • UP000103670 Componenti: Genome
  • UP000131569 Componenti: Genome
  • UP000143093 Componenti: Genome
  • UP000153467 Componenti: Genome
  • UP000148194 Componenti: Genome
  • UP000138690 Componenti: Genome
  • UP000109640 Componenti: Genome
  • UP000131955 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001060031 – 422NucleoproteinAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177Phosphoserine; by hostUniRule annotation1 Publication1

Post-translational modificationi

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues (By similarity). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis.UniRule annotation2 Publications

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

PRIDEiP59595

Interactioni

Subunit structurei

Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.UniRule annotation3 Publications

Protein-protein interaction databases

MINTiP59595

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 59Combined sources4
Beta strandi61 – 63Combined sources3
Beta strandi77 – 79Combined sources3
Helixi81 – 83Combined sources3
Beta strandi85 – 91Combined sources7
Beta strandi94 – 96Combined sources3
Turni98 – 100Combined sources3
Beta strandi102 – 104Combined sources3
Beta strandi108 – 113Combined sources6
Turni118 – 121Combined sources4
Beta strandi131 – 135Combined sources5
Beta strandi140 – 142Combined sources3
Turni145 – 147Combined sources3
Turni152 – 154Combined sources3
Beta strandi254 – 258Combined sources5
Helixi260 – 262Combined sources3
Helixi271 – 275Combined sources5
Turni282 – 284Combined sources3
Helixi290 – 295Combined sources6
Helixi296 – 298Combined sources3
Helixi302 – 306Combined sources5
Helixi312 – 318Combined sources7
Beta strandi319 – 325Combined sources7
Beta strandi330 – 340Combined sources11
Helixi347 – 357Combined sources11
Helixi360 – 363Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SSKNMR-A45-181[»]
1X7QX-ray1.45C362-370[»]
2CJRX-ray2.50A/B/C/D/E/F/G/H248-365[»]
2GIBX-ray1.75A/B270-370[»]
2JW8NMR-A/B248-365[»]
2OFZX-ray1.17A49-174[»]
2OG3X-ray1.85A49-174[»]
3I6LX-ray2.40F346-354[»]
DisProtiDP00948
ProteinModelPortaliP59595
SMRiP59595
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59595

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 187RNA-bindingUniRule annotationAdd BLAST146
Regioni45 – 181RNA-bindingAdd BLAST137
Regioni259 – 362DimerizationUniRule annotationAdd BLAST104

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 213Ser-richAdd BLAST33
Compositional biasi220 – 225Poly-Leu6
Compositional biasi240 – 245Poly-Gln6
Compositional biasi370 – 376Poly-Lys7

Sequence similaritiesi

Belongs to the betacoronavirus nucleocapsid protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900008T

Family and domain databases

HAMAPiMF_04096 BETA_CORONA_NCAP, 1 hit
InterProiView protein in InterPro
IPR001218 Corona_nucleocap
IPR037179 Nucleocapsid_C
IPR037195 Nucleocapsid_N
PfamiView protein in Pfam
PF00937 Corona_nucleoca, 1 hit
PIRSFiPIRSF003888 Corona_nucleocap, 1 hit
SUPFAMiSSF103068 SSF103068, 1 hit
SSF110304 SSF110304, 1 hit

Sequencei

Sequence statusi: Complete.

P59595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNGPQSNQ RSAPRITFGG PTDSTDNNQN GGRNGARPKQ RRPQGLPNNT
60 70 80 90 100
ASWFTALTQH GKEELRFPRG QGVPINTNSG PDDQIGYYRR ATRRVRGGDG
110 120 130 140 150
KMKELSPRWY FYYLGTGPEA SLPYGANKEG IVWVATEGAL NTPKDHIGTR
160 170 180 190 200
NPNNNAATVL QLPQGTTLPK GFYAEGSRGG SQASSRSSSR SRGNSRNSTP
210 220 230 240 250
GSSRGNSPAR MASGGGETAL ALLLLDRLNQ LESKVSGKGQ QQQGQTVTKK
260 270 280 290 300
SAAEASKKPR QKRTATKQYN VTQAFGRRGP EQTQGNFGDQ DLIRQGTDYK
310 320 330 340 350
HWPQIAQFAP SASAFFGMSR IGMEVTPSGT WLTYHGAIKL DDKDPQFKDN
360 370 380 390 400
VILLNKHIDA YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ PTVTLLPAAD
410 420
MDDFSRQLQN SMSGASADST QA
Length:422
Mass (Da):46,025
Last modified:April 23, 2003 - v1
Checksum:i43FC8750F1253034
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti50T → I in strain: Isolate Frankfurt 1 and Isolate FRA. 1
Natural varianti154N → Y in strain: Isolate BJ03. 1
Natural varianti193G → C in strain: Isolate CUHK-Su10. 1
Natural varianti325 – 326VT → AA in strain: Isolate Shanghai LY. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY278741 Genomic RNA Translation: AAP13445.1
AY274119 Genomic RNA Translation: AAP41047.1
AY278554 Genomic RNA Translation: AAP13814.1
AY282752 Genomic RNA Translation: AAP30714.1
AY304492 Genomic RNA No translation available.
AY304495 Genomic RNA No translation available.
AY278491 Genomic RNA No translation available.
AY283794 Genomic RNA No translation available.
AY283795 Genomic RNA No translation available.
AY283796 Genomic RNA No translation available.
AY283797 Genomic RNA No translation available.
AY278487 Genomic RNA No translation available.
AY278488 Genomic RNA Translation: AAP30037.1
AY278489 Genomic RNA Translation: AAP51234.1
AY278490 Genomic RNA No translation available.
AY279354 Genomic RNA No translation available.
AY291451 Genomic RNA Translation: AAP37024.1
AY310120 Genomic RNA Translation: AAP50495.1
AY291315 Genomic RNA Translation: AAP33707.1
AY307165 Genomic RNA Translation: AAP49024.1
AY290752 Genomic RNA Translation: AAP44772.1
AY321118 Genomic RNA No translation available.
AY322208 Genomic RNA Translation: AAP82974.1
AY338174 Genomic RNA Translation: AAQ01605.1
AY338175 Genomic RNA Translation: AAQ01617.1
AY348314 Genomic RNA Translation: AAP97890.1
AP006557 Genomic RNA Translation: BAC81358.1
AP006558 Genomic RNA Translation: BAC81372.1
AP006559 Genomic RNA Translation: BAC81386.1
AP006560 Genomic RNA Translation: BAC81400.1
AP006561 Genomic RNA Translation: BAC81414.1
AY323977 Genomic RNA Translation: AAP72984.1
AY362698 Genomic RNA No translation available.
AY362699 Genomic RNA No translation available.
AY427439 Genomic RNA Translation: AAQ94070.1
RefSeqiNP_828858.1, NC_004718.3

Genome annotation databases

GeneIDi1489678
KEGGivg:1489678

Similar proteinsi

Entry informationi

Entry nameiNCAP_CVHSA
AccessioniPrimary (citable) accession number: P59595
Secondary accession number(s): Q7T3Z4
, Q7TA14, Q7TF99, Q80E50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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