Reviewed, UniProtKB/Swiss-Prot P59576 (CARA_BUCBP)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	bbp_135

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112263
Regions
	Domain	204 – 391	188	Glutamine amidotransferase type-1
	Region	1 – 200	200	CPSase HAMAP MF_01209
Sites
	Active site	280	1	Nucleophile By similarity
	Active site	364	1	By similarity
	Active site	366	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59576-1 [UniParc]. Last modified April 11, 2003. Version 1. Checksum: 2EA405CB4D5E240D	FASTA	397	44,774
	10 20 30 40 50 60 MKHVLRKEKT LEISATLVLK DGTIFYGKSV GIQGETYGEI VFNTSMTGYQ EILTDPSYSN 70 80 90 100 110 120 QIITFTYPHI GNVGINKNDC ESNVIHAKGL IVRDISSIYS NHRSQMSLLN YLLNQKIIVI 130 140 150 160 170 180 SNIDTRKLTR ILRTTGSQYG YITTKKIHSI INVLKHINIL DNPITNKDLV KQVSTKKFYI 190 200 210 220 230 240 WKKNVNKQKN NNITNAKLKE KIWHVVVYDF GVKKNILNML THRNCYLTVI PADTSAEKVL 250 260 270 280 290 300 QILPDGIFLS NGPGDPRSCT YALNAINAFL KINIPIFGIC LGHQLLALAS GAKIIKMKFG 310 320 330 340 350 360 HHGSNHPVKE LKSNTVMITS QNHNYTVDTN NLPKNINITH ISLFDGSIQG LQRNDKHAFS 370 380 390 FQGHPEASPG PHDSMILFDK FIKLMKKSYN SQQKTII

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26869.1.
RefSeq	NP_777764.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1058148.
GenomeReviews	Gene locus bbp_135 in contig AE016826_GR.
KEGG	bab:bbp135.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59576.
OMA	P59576. LFDGSNC.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_135-MON.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BUCBP

Accession

Primary (citable) accession number: P59576

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	April 11, 2003
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)