ID   T2R60_HUMAN             Reviewed;         318 AA.
AC   P59551; A4D2G8; Q645W8; Q7RTR7;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   11-APR-2003, sequence version 1.
DT   24-JAN-2024, entry version 154.
DE   RecName: Full=Taste receptor type 2 member 60;
DE            Short=T2R60;
DE   AltName: Full=Taste receptor type 2 member 56;
DE            Short=T2R56;
GN   Name=TAS2R60;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12584440; DOI=10.1159/000068546;
RA   Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.;
RT   "Identification and characterization of human taste receptor genes
RT   belonging to the TAS2R family.";
RL   Cytogenet. Genome Res. 98:45-53(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15496549; DOI=10.1093/molbev/msi027;
RA   Fischer A., Gilad Y., Man O., Paeaebo S.;
RT   "Evolution of bitter taste receptors in humans and apes.";
RL   Mol. Biol. Evol. 22:432-436(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA   Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA   Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA   Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA   Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA   Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA   Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA   Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA   Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA   Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA   Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA   Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA   Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=12679530; DOI=10.1093/molbev/msg083;
RA   Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT   "Adaptive diversification of bitter taste receptor genes in mammalian
RT   evolution.";
RL   Mol. Biol. Evol. 20:805-814(2003).
RN   [6]
RP   REVIEW.
RX   PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA   Montmayeur J.-P., Matsunami H.;
RT   "Receptors for bitter and sweet taste.";
RL   Curr. Opin. Neurobiol. 12:366-371(2002).
RN   [7]
RP   REVIEW.
RX   PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA   Margolskee R.F.;
RT   "Molecular mechanisms of bitter and sweet taste transduction.";
RL   J. Biol. Chem. 277:1-4(2002).
RN   [8]
RP   REVIEW.
RX   PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA   Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA   Zuker C.S., Ryba N.J.;
RT   "Coding of sweet, bitter, and umami tastes: different receptor cells
RT   sharing similar signaling pathways.";
RL   Cell 112:293-301(2003).
CC   -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC       and is gustducin-linked. May play a role in sensing the chemical
CC       composition of the gastrointestinal content. The activity of this
CC       receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC       and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC       tongue and exclusively in gustducin-positive cells.
CC   -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC       bitter compounds; individual taste cells can discriminate among bitter
CC       stimuli.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000305}.
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DR   EMBL; AY114094; AAM63544.1; -; Genomic_DNA.
DR   EMBL; AY724955; AAU21151.1; -; Genomic_DNA.
DR   EMBL; CH236959; EAL23790.1; -; Genomic_DNA.
DR   EMBL; BC100938; AAI00939.1; -; mRNA.
DR   EMBL; BC100939; AAI00940.1; -; mRNA.
DR   EMBL; BC100940; AAI00941.1; -; mRNA.
DR   EMBL; BK001100; DAA01207.1; -; Genomic_DNA.
DR   CCDS; CCDS5885.1; -.
DR   RefSeq; NP_803186.1; NM_177437.1.
DR   AlphaFoldDB; P59551; -.
DR   SMR; P59551; -.
DR   BioGRID; 130733; 4.
DR   IntAct; P59551; 3.
DR   STRING; 9606.ENSP00000327724; -.
DR   BindingDB; P59551; -.
DR   ChEMBL; CHEMBL3309109; -.
DR   GlyCosmos; P59551; 1 site, No reported glycans.
DR   GlyGen; P59551; 1 site.
DR   PhosphoSitePlus; P59551; -.
DR   BioMuta; TAS2R60; -.
DR   DMDM; 29839489; -.
DR   MassIVE; P59551; -.
DR   PaxDb; 9606-ENSP00000327724; -.
DR   PeptideAtlas; P59551; -.
DR   ProteomicsDB; 57152; -.
DR   Antibodypedia; 32663; 45 antibodies from 14 providers.
DR   DNASU; 338398; -.
DR   Ensembl; ENST00000332690.1; ENSP00000327724.1; ENSG00000185899.1.
DR   Ensembl; ENST00000642941.1; ENSP00000494922.1; ENSG00000285341.1.
DR   GeneID; 338398; -.
DR   KEGG; hsa:338398; -.
DR   MANE-Select; ENST00000332690.1; ENSP00000327724.1; NM_177437.1; NP_803186.1.
DR   UCSC; uc011ktg.2; human.
DR   AGR; HGNC:20639; -.
DR   CTD; 338398; -.
DR   DisGeNET; 338398; -.
DR   GeneCards; TAS2R60; -.
DR   HGNC; HGNC:20639; TAS2R60.
DR   HPA; ENSG00000185899; Not detected.
DR   MIM; 613968; gene.
DR   neXtProt; NX_P59551; -.
DR   PharmGKB; PA134879120; -.
DR   VEuPathDB; HostDB:ENSG00000185899; -.
DR   eggNOG; ENOG502S2SI; Eukaryota.
DR   GeneTree; ENSGT01100000263477; -.
DR   HOGENOM; CLU_072337_1_1_1; -.
DR   InParanoid; P59551; -.
DR   OMA; FCLQWVV; -.
DR   OrthoDB; 4642354at2759; -.
DR   PhylomeDB; P59551; -.
DR   TreeFam; TF335891; -.
DR   PathwayCommons; P59551; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   SignaLink; P59551; -.
DR   BioGRID-ORCS; 338398; 12 hits in 1137 CRISPR screens.
DR   GeneWiki; TAS2R60; -.
DR   GenomeRNAi; 338398; -.
DR   Pharos; P59551; Tchem.
DR   PRO; PR:P59551; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; P59551; Protein.
DR   Bgee; ENSG00000185899; Expressed in primordial germ cell in gonad and 16 other cell types or tissues.
DR   GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; NAS:UniProtKB.
DR   GO; GO:0050913; P:sensory perception of bitter taste; NAS:UniProtKB.
DR   CDD; cd15018; 7tm_TAS2R41-like; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR007960; TAS2R.
DR   PANTHER; PTHR11394; TASTE RECEPTOR TYPE 2; 1.
DR   PANTHER; PTHR11394:SF32; TASTE RECEPTOR TYPE 2 MEMBER 60; 1.
DR   Pfam; PF05296; TAS2R; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
PE   2: Evidence at transcript level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..318
FT                   /note="Taste receptor type 2 member 60"
FT                   /id="PRO_0000082351"
FT   TOPO_DOM        1..7
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..40
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..88
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..128
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        150..183
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        205..234
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        256..264
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        265..285
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        286..318
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        179
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   318 AA;  36337 MW;  DEDAB1CC8101764B CRC64;
     MNGDHMVLGS SVTDKKAIIL VTILLLLRLV AIAGNGFITA ALGVEWVLRR MLLPCDKLLV
     SLGASRFCLQ SVVMGKTIYV FLHPMAFPYN PVLQFLAFQW DFLNAATLWS STWLSVFYCV
     KIATFTHPVF FWLKHKLSGW LPWMLFSSVG LSSFTTILFF IGNHRMYQNY LRNHLQPWNV
     TGDSIRSYCE KFYLFPLKMI TWTMPTAVFF ICMILLITSL GRHRKKALLT TSGFREPSVQ
     AHIKALLALL SFAMLFISYF LSLVFSAAGI FPPLDFKFWV WESVIYLCAA VHPIILLFSN
     CRLRAVLKSR RSSRCGTP
//