ID   T2R50_HUMAN             Reviewed;         299 AA.
AC   P59544; P59545; Q2M255; Q645Y0;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   24-JAN-2024, entry version 153.
DE   RecName: Full=Taste receptor type 2 member 50;
DE            Short=T2R50;
DE   AltName: Full=Taste receptor type 2 member 51;
DE            Short=T2R51;
GN   Name=TAS2R50;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT TYR-203.
RX   PubMed=12379855; DOI=10.1038/ng1014;
RA   Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.;
RT   "The human TAS2R16 receptor mediates bitter taste in response to beta-
RT   glucopyranosides.";
RL   Nat. Genet. 32:397-401(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12584440; DOI=10.1159/000068546;
RA   Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.;
RT   "Identification and characterization of human taste receptor genes
RT   belonging to the TAS2R family.";
RL   Cytogenet. Genome Res. 98:45-53(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15496549; DOI=10.1093/molbev/msi027;
RA   Fischer A., Gilad Y., Man O., Paeaebo S.;
RT   "Evolution of bitter taste receptors in humans and apes.";
RL   Mol. Biol. Evol. 22:432-436(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA   Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA   Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA   Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA   Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA   Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA   Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA   Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA   Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA   Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA   Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA   Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA   Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA   David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA   D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA   Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA   Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA   Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA   LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA   Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA   Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA   Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA   Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA   Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA   Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA   Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA   Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA   Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA   Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA   Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   REVIEW.
RX   PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA   Montmayeur J.-P., Matsunami H.;
RT   "Receptors for bitter and sweet taste.";
RL   Curr. Opin. Neurobiol. 12:366-371(2002).
RN   [7]
RP   REVIEW.
RX   PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA   Margolskee R.F.;
RT   "Molecular mechanisms of bitter and sweet taste transduction.";
RL   J. Biol. Chem. 277:1-4(2002).
RN   [8]
RP   REVIEW.
RX   PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA   Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA   Zuker C.S., Ryba N.J.;
RT   "Coding of sweet, bitter, and umami tastes: different receptor cells
RT   sharing similar signaling pathways.";
RL   Cell 112:293-301(2003).
CC   -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC       and is gustducin-linked. May play a role in sensing the chemical
CC       composition of the gastrointestinal content. The activity of this
CC       receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC       and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC       tongue and exclusively in gustducin-positive cells.
CC   -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC       bitter compounds; individual taste cells can discriminate among bitter
CC       stimuli.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000305}.
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DR   EMBL; AF494235; AAM19326.1; -; Genomic_DNA.
DR   EMBL; AY114088; AAM63538.1; -; Genomic_DNA.
DR   EMBL; AY724937; AAU21139.1; -; Genomic_DNA.
DR   EMBL; AC018630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC112100; AAI12101.1; -; mRNA.
DR   CCDS; CCDS8638.1; -.
DR   RefSeq; NP_795371.2; NM_176890.2.
DR   AlphaFoldDB; P59544; -.
DR   SMR; P59544; -.
DR   BioGRID; 129251; 26.
DR   STRING; 9606.ENSP00000424040; -.
DR   ChEMBL; CHEMBL3559706; -.
DR   GuidetoPHARMACOLOGY; 677; -.
DR   GlyCosmos; P59544; 1 site, No reported glycans.
DR   GlyGen; P59544; 1 site.
DR   BioMuta; TAS2R50; -.
DR   DMDM; 296452919; -.
DR   PaxDb; 9606-ENSP00000424040; -.
DR   Antibodypedia; 23416; 51 antibodies from 15 providers.
DR   DNASU; 259296; -.
DR   Ensembl; ENST00000506868.1; ENSP00000424040.1; ENSG00000212126.3.
DR   Ensembl; ENST00000575654.1; ENSP00000458551.1; ENSG00000273431.1.
DR   Ensembl; ENST00000613831.1; ENSP00000479785.1; ENSG00000276167.1.
DR   GeneID; 259296; -.
DR   KEGG; hsa:259296; -.
DR   MANE-Select; ENST00000506868.1; ENSP00000424040.1; NM_176890.2; NP_795371.2.
DR   UCSC; uc001qzl.2; human.
DR   AGR; HGNC:18882; -.
DR   CTD; 259296; -.
DR   DisGeNET; 259296; -.
DR   GeneCards; TAS2R50; -.
DR   HGNC; HGNC:18882; TAS2R50.
DR   HPA; ENSG00000212126; Not detected.
DR   MIM; 609627; gene.
DR   neXtProt; NX_P59544; -.
DR   OpenTargets; ENSG00000212126; -.
DR   PharmGKB; PA38736; -.
DR   VEuPathDB; HostDB:ENSG00000212126; -.
DR   eggNOG; ENOG502TE6U; Eukaryota.
DR   GeneTree; ENSGT01100000263477; -.
DR   HOGENOM; CLU_072337_2_0_1; -.
DR   InParanoid; P59544; -.
DR   OMA; ILCQIRC; -.
DR   OrthoDB; 5306466at2759; -.
DR   PhylomeDB; P59544; -.
DR   TreeFam; TF335891; -.
DR   PathwayCommons; P59544; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR   BioGRID-ORCS; 259296; 12 hits in 1101 CRISPR screens.
DR   GeneWiki; TAS2R50; -.
DR   GenomeRNAi; 259296; -.
DR   Pharos; P59544; Tchem.
DR   PRO; PR:P59544; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; P59544; Protein.
DR   Bgee; ENSG00000212126; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 34 other cell types or tissues.
DR   GO; GO:0016020; C:membrane; IC:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR   CDD; cd15027; 7tm_TAS2R43-like; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR007960; TAS2R.
DR   PANTHER; PTHR11394; TASTE RECEPTOR TYPE 2; 1.
DR   PANTHER; PTHR11394:SF43; TASTE RECEPTOR TYPE 2 MEMBER 50; 1.
DR   Pfam; PF05296; TAS2R; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   Genevisible; P59544; HS.
PE   2: Evidence at transcript level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..299
FT                   /note="Taste receptor type 2 member 50"
FT                   /id="PRO_0000082337"
FT   TOPO_DOM        1
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        2..22
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..55
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..87
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        109..126
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..181
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..229
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        251..259
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        281..299
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        161
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         203
FT                   /note="C -> Y (in dbSNP:rs1376251)"
FT                   /evidence="ECO:0000269|PubMed:12379855"
FT                   /id="VAR_024187"
SQ   SEQUENCE   299 AA;  34558 MW;  D5C3E41E1B370E6A CRC64;
     MITFLYIFFS ILIMVLFVLG NFANGFIALV NFIDWVKRKK ISSADQILTA LAVSRIGLLW
     ALLLNWYLTV LNPAFYSVEL RITSYNAWVV TNHFSMWLAA NLSIFYLLKI ANFSNLLFLH
     LKRRVRSVIL VILLGTLIFL VCHLLVANMD ESMWAEEYEG NMTGKMKLRN TVHLSYLTVT
     TLWSFIPFTL SLISFLMLIC SLCKHLKKMQ LHGEGSQDLS TKVHIKALQT LISFLLLCAI
     FFLFLIVSVW SPRRLRNDPV VMVSKAVGNI YLAFDSFILI WRTKKLKHTF LLILCQIRC
//