P59544 (T2R50_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 50
Short name=T2R50

Alternative name(s):
Taste receptor type 2 member 51
Short name=T2R51

Gene names

Name:

TAS2R50

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.
Miscellaneous	Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sensory perception of taste Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Taste receptor type 2 member 50		PRO_0000082337
Regions
	Topological domain	1	1	Extracellular Potential
	Transmembrane	2 – 22	21	Helical; Name=1; Potential
	Topological domain	23 – 55	33	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Helical; Name=2; Potential
	Topological domain	77 – 87	11	Extracellular Potential
	Transmembrane	88 – 108	21	Helical; Name=3; Potential
	Topological domain	109 – 126	18	Cytoplasmic Potential
	Transmembrane	127 – 147	21	Helical; Name=4; Potential
	Topological domain	148 – 181	34	Extracellular Potential
	Transmembrane	182 – 202	21	Helical; Name=5; Potential
	Topological domain	203 – 229	27	Cytoplasmic Potential
	Transmembrane	230 – 250	21	Helical; Name=6; Potential
	Topological domain	251 – 259	9	Extracellular Potential
	Transmembrane	260 – 280	21	Helical; Name=7; Potential
	Topological domain	281 – 299	19	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	203	1	C → Y. Ref.1 Corresponds to variant rs1376251 [ dbSNP | Ensembl ].		VAR_024187

Sequences

	Sequence		Length	Mass (Da)
	P59544 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: D5C3E41E1B370E6A	FASTA	299	34,558
	10 20 30 40 50 60 MITFLYIFFS ILIMVLFVLG NFANGFIALV NFIDWVKRKK ISSADQILTA LAVSRIGLLW 70 80 90 100 110 120 ALLLNWYLTV LNPAFYSVEL RITSYNAWVV TNHFSMWLAA NLSIFYLLKI ANFSNLLFLH 130 140 150 160 170 180 LKRRVRSVIL VILLGTLIFL VCHLLVANMD ESMWAEEYEG NMTGKMKLRN TVHLSYLTVT 190 200 210 220 230 240 TLWSFIPFTL SLISFLMLIC SLCKHLKKMQ LHGEGSQDLS TKVHIKALQT LISFLLLCAI 250 260 270 280 290 FFLFLIVSVW SPRRLRNDPV VMVSKAVGNI YLAFDSFILI WRTKKLKHTF LLILCQIRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W. Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TYR-203.
[2]	"Identification and characterization of human taste receptor genes belonging to the TAS2R family." Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J. Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[8]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF494235 Genomic DNA. Translation: AAM19326.1. AY114088 Genomic DNA. Translation: AAM63538.1. AY724937 Genomic DNA. Translation: AAU21139.1. AC018630 Genomic DNA. No translation available. BC112100 mRNA. Translation: AAI12101.1.
IPI	IPI00259693.
RefSeq	NP_795371.2. NM_176890.2.
UniGene	Hs.688194.
3D structure databases
ProteinModelPortal	P59544.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000375093.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	296452919.
Proteomic databases
PaxDb	P59544.
PRIDE	P59544.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000506868; ENSP00000424040; ENSG00000212126. ENST00000570541; ENSP00000458427; ENSG00000269596. ENST00000575654; ENSP00000458551; ENSG00000269869.
GeneID	259296.
KEGG	hsa:259296.
UCSC	uc001qzl.2. human.
Organism-specific databases
CTD	259296.
GeneCards	GC12M011138.
HGNC	HGNC:18882. TAS2R50.
MIM	609627. gene.
neXtProt	NX_P59544.
PharmGKB	PA38736.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG74690.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
InParanoid	P59544.
KO	K08474.
OMA	FILIWRT.
PhylomeDB	P59544.
Gene expression databases
ArrayExpress	P59544.
Bgee	P59544.
CleanEx	HS_TAS2R50.
Genevestigator	P59544.
Family and domain databases
InterPro	IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	259296.
NextBio	93145.
SOURCE	Search...

Entry information

Entry name

T2R50_HUMAN

Accession

Primary (citable) accession number: P59544
Secondary accession number(s): P59545, Q2M255, Q645Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.