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P59544 (T2R50_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 50

Short name=T2R50
Alternative name(s):
Taste receptor type 2 member 51
Short name=T2R51
Gene names
Name:TAS2R50
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.

Miscellaneous

Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
   Biological processSensory transduction
Taste
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsensory perception of taste

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Taste receptor type 2 member 50
PRO_0000082337

Regions

Topological domain11Extracellular Potential
Transmembrane2 – 2221Helical; Name=1; Potential
Topological domain23 – 5533Cytoplasmic Potential
Transmembrane56 – 7621Helical; Name=2; Potential
Topological domain77 – 8711Extracellular Potential
Transmembrane88 – 10821Helical; Name=3; Potential
Topological domain109 – 12618Cytoplasmic Potential
Transmembrane127 – 14721Helical; Name=4; Potential
Topological domain148 – 18134Extracellular Potential
Transmembrane182 – 20221Helical; Name=5; Potential
Topological domain203 – 22927Cytoplasmic Potential
Transmembrane230 – 25021Helical; Name=6; Potential
Topological domain251 – 2599Extracellular Potential
Transmembrane260 – 28021Helical; Name=7; Potential
Topological domain281 – 29919Cytoplasmic Potential

Amino acid modifications

Glycosylation1611N-linked (GlcNAc...) Potential

Natural variations

Natural variant2031C → Y. Ref.1
Corresponds to variant rs1376251 [ dbSNP | Ensembl ].
VAR_024187

Sequences

Sequence LengthMass (Da)Tools
P59544 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: D5C3E41E1B370E6A

FASTA29934,558
        10         20         30         40         50         60 
MITFLYIFFS ILIMVLFVLG NFANGFIALV NFIDWVKRKK ISSADQILTA LAVSRIGLLW 

        70         80         90        100        110        120 
ALLLNWYLTV LNPAFYSVEL RITSYNAWVV TNHFSMWLAA NLSIFYLLKI ANFSNLLFLH 

       130        140        150        160        170        180 
LKRRVRSVIL VILLGTLIFL VCHLLVANMD ESMWAEEYEG NMTGKMKLRN TVHLSYLTVT 

       190        200        210        220        230        240 
TLWSFIPFTL SLISFLMLIC SLCKHLKKMQ LHGEGSQDLS TKVHIKALQT LISFLLLCAI 

       250        260        270        280        290 
FFLFLIVSVW SPRRLRNDPV VMVSKAVGNI YLAFDSFILI WRTKKLKHTF LLILCQIRC 

« Hide

References

« Hide 'large scale' references
[1]"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides."
Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.
Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT TYR-203.
[2]"Identification and characterization of human taste receptor genes belonging to the TAS2R family."
Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.
Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF494235 Genomic DNA. Translation: AAM19326.1.
AY114088 Genomic DNA. Translation: AAM63538.1.
AY724937 Genomic DNA. Translation: AAU21139.1.
AC018630 Genomic DNA. No translation available.
BC112100 mRNA. Translation: AAI12101.1.
RefSeqNP_795371.2. NM_176890.2.
UniGeneHs.688194.

3D structure databases

ProteinModelPortalP59544.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000375093.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM296452919.

Proteomic databases

PaxDbP59544.
PRIDEP59544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000506868; ENSP00000424040; ENSG00000212126.
ENST00000570541; ENSP00000458427; ENSG00000272952.
ENST00000575654; ENSP00000458551; ENSG00000273431.
GeneID259296.
KEGGhsa:259296.
UCSCuc001qzl.2. human.

Organism-specific databases

CTD259296.
GeneCardsGC12M011138.
HGNCHGNC:18882. TAS2R50.
MIM609627. gene.
neXtProtNX_P59544.
PharmGKBPA38736.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG74690.
HOGENOMHOG000234595.
HOVERGENHBG060698.
InParanoidP59544.
KOK08474.
OMADSFILIW.
OrthoDBEOG7P02JB.
PhylomeDBP59544.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP59544.
BgeeP59544.
CleanExHS_TAS2R50.
GenevestigatorP59544.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR017452. GPCR_Rhodpsn_7TM.
IPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R50.
GenomeRNAi259296.
NextBio93145.
PROP59544.
SOURCESearch...

Entry information

Entry nameT2R50_HUMAN
AccessionPrimary (citable) accession number: P59544
Secondary accession number(s): P59545, Q2M255, Q645Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries