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P59543

- T2R20_HUMAN

UniProt

P59543 - T2R20_HUMAN

Protein

Taste receptor type 2 member 20

Gene

TAS2R20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.By similarity

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW

    GO - Biological processi

    1. sensory perception of taste Source: UniProtKB-KW

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Sensory transduction, Taste

    Enzyme and pathway databases

    ReactomeiREACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Taste receptor type 2 member 20
    Alternative name(s):
    Taste receptor type 2 member 49
    Short name:
    T2R49
    Taste receptor type 2 member 56
    Short name:
    T2R56
    Gene namesi
    Name:TAS2R20
    Synonyms:TAS2R49
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:19109. TAS2R20.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Taste receptor type 2 member 20PRO_0000082331Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP59543.
    PRIDEiP59543.

    PTM databases

    PhosphoSiteiP59543.

    Expressioni

    Tissue specificityi

    Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.

    Gene expression databases

    ArrayExpressiP59543.
    CleanExiHS_TAS2R49.
    GenevestigatoriP59543.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000375092.

    Structurei

    3D structure databases

    ProteinModelPortaliP59543.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66ExtracellularSequence Analysis
    Topological domaini28 – 4619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini68 – 7912ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini101 – 12525CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini147 – 17832ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini200 – 22930CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini251 – 2599ExtracellularSequence Analysis
    Topological domaini281 – 30929CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei47 – 6721Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei80 – 10021Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei126 – 14621Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei179 – 19921Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43462.
    HOGENOMiHOG000234595.
    HOVERGENiHBG060698.
    InParanoidiP59543.
    KOiK08474.
    OMAiPSTKIHI.
    OrthoDBiEOG7P02JB.
    PhylomeDBiP59543.
    TreeFamiTF335891.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR017452. GPCR_Rhodpsn_7TM.
    IPR007960. TAS2_rcpt.
    [Graphical view]
    PfamiPF05296. TAS2R. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59543-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSFLHIVFS ILVVVAFILG NFANGFIALI NFIAWVKRQK ISSADQIIAA    50
    LAVSRVGLLW VILLHWYSTV LNPTSSNLKV IIFISNAWAV TNHFSIWLAT 100
    SLSIFYLLKI VNFSRLIFHH LKRKAKSVVL VIVLGSLFFL VCHLVMKHTY 150
    INVWTEECEG NVTWKIKLRN AMHLSNLTVA MLANLIPFTL TLISFLLLIY 200
    SLCKHLKKMQ LHGKGSQDPS TKIHIKALQT VTSFLILLAI YFLCLIISFW 250
    NFKMRPKEIV LMLCQAFGII YPSFHSFILI WGNKTLKQTF LSVLWQVTCW 300
    AKGQNQSTP 309
    Length:309
    Mass (Da):35,358
    Last modified:November 23, 2004 - v2
    Checksum:iAB200710EE19361C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331I → V in AAU21140. (PubMed:15496549)Curated
    Sequence conflicti55 – 551R → K in AAM19327. (PubMed:12379855)Curated
    Sequence conflicti141 – 1433VCH → ICQ in AAU21140. (PubMed:15496549)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti79 – 791K → E.1 Publication
    Corresponds to variant rs7135018 [ dbSNP | Ensembl ].
    VAR_053356
    Natural varianti143 – 1431H → Q.
    Corresponds to variant rs12226920 [ dbSNP | Ensembl ].
    VAR_053357
    Natural varianti148 – 1481H → N.1 Publication
    Corresponds to variant rs12226919 [ dbSNP | Ensembl ].
    VAR_053358
    Natural varianti236 – 2361I → V.1 Publication
    Corresponds to variant rs10845281 [ dbSNP | Ensembl ].
    VAR_053359
    Natural varianti252 – 2521F → S.1 Publication
    Corresponds to variant rs10845280 [ dbSNP | Ensembl ].
    VAR_053360
    Natural varianti255 – 2551R → L.1 Publication
    Corresponds to variant rs10845279 [ dbSNP | Ensembl ].
    VAR_053361

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF494236 Genomic DNA. Translation: AAM19327.1.
    AY114092 Genomic DNA. Translation: AAM63542.1.
    AY724938 Genomic DNA. Translation: AAU21140.1.
    BC100915 mRNA. Translation: AAI00916.1.
    BC100916 mRNA. Translation: AAI00917.1.
    BC100918 mRNA. Translation: AAI00919.1.
    BC113843 mRNA. Translation: AAI13844.1.
    CCDSiCCDS8639.1.
    RefSeqiNP_795370.2. NM_176889.2.
    UniGeneiHs.686384.

    Genome annotation databases

    EnsembliENST00000538986; ENSP00000441624; ENSG00000255837.
    ENST00000570941; ENSP00000459353; ENSG00000273092.
    GeneIDi259295.
    KEGGihsa:259295.
    UCSCiuc001qzm.2. human.

    Polymorphism databases

    DMDMi55977811.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF494236 Genomic DNA. Translation: AAM19327.1 .
    AY114092 Genomic DNA. Translation: AAM63542.1 .
    AY724938 Genomic DNA. Translation: AAU21140.1 .
    BC100915 mRNA. Translation: AAI00916.1 .
    BC100916 mRNA. Translation: AAI00917.1 .
    BC100918 mRNA. Translation: AAI00919.1 .
    BC113843 mRNA. Translation: AAI13844.1 .
    CCDSi CCDS8639.1.
    RefSeqi NP_795370.2. NM_176889.2.
    UniGenei Hs.686384.

    3D structure databases

    ProteinModelPortali P59543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000375092.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P59543.

    Polymorphism databases

    DMDMi 55977811.

    Proteomic databases

    PaxDbi P59543.
    PRIDEi P59543.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000538986 ; ENSP00000441624 ; ENSG00000255837 .
    ENST00000570941 ; ENSP00000459353 ; ENSG00000273092 .
    GeneIDi 259295.
    KEGGi hsa:259295.
    UCSCi uc001qzm.2. human.

    Organism-specific databases

    CTDi 259295.
    GeneCardsi GC12M011148.
    HGNCi HGNC:19109. TAS2R20.
    MIMi 613962. gene.
    neXtProti NX_P59543.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43462.
    HOGENOMi HOG000234595.
    HOVERGENi HBG060698.
    InParanoidi P59543.
    KOi K08474.
    OMAi PSTKIHI.
    OrthoDBi EOG7P02JB.
    PhylomeDBi P59543.
    TreeFami TF335891.

    Enzyme and pathway databases

    Reactomei REACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    GeneWikii TAS2R20.
    GenomeRNAii 259295.
    NextBioi 93141.
    PROi P59543.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P59543.
    CleanExi HS_TAS2R49.
    Genevestigatori P59543.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR017452. GPCR_Rhodpsn_7TM.
    IPR007960. TAS2_rcpt.
    [Graphical view ]
    Pfami PF05296. TAS2R. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides."
      Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.
      Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification and characterization of human taste receptor genes belonging to the TAS2R family."
      Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.
      Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Evolution of bitter taste receptors in humans and apes."
      Fischer A., Gilad Y., Man O., Paeaebo S.
      Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-148; VAL-236; SER-252 AND LEU-255.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-79.
    5. Cited for: REVIEW.
    6. "Molecular mechanisms of bitter and sweet taste transduction."
      Margolskee R.F.
      J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
      Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
      Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiT2R20_HUMAN
    AccessioniPrimary (citable) accession number: P59543
    Secondary accession number(s): P59549
    , Q2HIZ4, Q496D8, Q645X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 11, 2003
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3