P59543 (T2R20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 20

Alternative name(s):
Taste receptor type 2 member 49
Short name=T2R49
Taste receptor type 2 member 56
Short name=T2R56

Gene names

Name:	TAS2R20
Synonyms:	TAS2R49

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.
Miscellaneous	Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sensory perception of taste Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Taste receptor type 2 member 20		PRO_0000082331
Regions
	Topological domain	1 – 6	6	Extracellular Potential
	Transmembrane	7 – 27	21	Helical; Name=1; Potential
	Topological domain	28 – 46	19	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Helical; Name=2; Potential
	Topological domain	68 – 79	12	Extracellular Potential
	Transmembrane	80 – 100	21	Helical; Name=3; Potential
	Topological domain	101 – 125	25	Cytoplasmic Potential
	Transmembrane	126 – 146	21	Helical; Name=4; Potential
	Topological domain	147 – 178	32	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Name=5; Potential
	Topological domain	200 – 229	30	Cytoplasmic Potential
	Transmembrane	230 – 250	21	Helical; Name=6; Potential
	Topological domain	251 – 259	9	Extracellular Potential
	Transmembrane	260 – 280	21	Helical; Name=7; Potential
	Topological domain	281 – 309	29	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	79	1	K → E. Ref.4 Corresponds to variant rs7135018 [ dbSNP | Ensembl ].		VAR_053356
	Natural variant	143	1	H → Q. Corresponds to variant rs12226920 [ dbSNP | Ensembl ].		VAR_053357
	Natural variant	148	1	H → N. Ref.3 Corresponds to variant rs12226919 [ dbSNP | Ensembl ].		VAR_053358
	Natural variant	236	1	I → V. Ref.3 Corresponds to variant rs10845281 [ dbSNP | Ensembl ].		VAR_053359
	Natural variant	252	1	F → S. Ref.3 Corresponds to variant rs10845280 [ dbSNP | Ensembl ].		VAR_053360
	Natural variant	255	1	R → L. Ref.3 Corresponds to variant rs10845279 [ dbSNP | Ensembl ].		VAR_053361
Experimental info
	Sequence conflict	33	1	I → V in AAU21140. Ref.3
	Sequence conflict	55	1	R → K in AAM19327. Ref.1
	Sequence conflict	141 – 143	3	VCH → ICQ in AAU21140. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P59543 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: AB200710EE19361C	FASTA	309	35,358
	10 20 30 40 50 60 MMSFLHIVFS ILVVVAFILG NFANGFIALI NFIAWVKRQK ISSADQIIAA LAVSRVGLLW 70 80 90 100 110 120 VILLHWYSTV LNPTSSNLKV IIFISNAWAV TNHFSIWLAT SLSIFYLLKI VNFSRLIFHH 130 140 150 160 170 180 LKRKAKSVVL VIVLGSLFFL VCHLVMKHTY INVWTEECEG NVTWKIKLRN AMHLSNLTVA 190 200 210 220 230 240 MLANLIPFTL TLISFLLLIY SLCKHLKKMQ LHGKGSQDPS TKIHIKALQT VTSFLILLAI 250 260 270 280 290 300 YFLCLIISFW NFKMRPKEIV LMLCQAFGII YPSFHSFILI WGNKTLKQTF LSVLWQVTCW AKGQNQSTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W. Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification and characterization of human taste receptor genes belonging to the TAS2R family." Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J. Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-148; VAL-236; SER-252 AND LEU-255.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-79.
[5]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[6]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF494236 Genomic DNA. Translation: AAM19327.1. AY114092 Genomic DNA. Translation: AAM63542.1. AY724938 Genomic DNA. Translation: AAU21140.1. BC100915 mRNA. Translation: AAI00916.1. BC100916 mRNA. Translation: AAI00917.1. BC100918 mRNA. Translation: AAI00919.1. BC113843 mRNA. Translation: AAI13844.1.
IPI	IPI00255111.
RefSeq	NP_795370.2. NM_176889.2.
UniGene	Hs.686384.
3D structure databases
ProteinModelPortal	P59543.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000375092.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P59543.
Polymorphism databases
DMDM	55977811.
Proteomic databases
PaxDb	P59543.
PRIDE	P59543.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000538986; ENSP00000441624; ENSG00000255837. ENST00000570941; ENSP00000459353; ENSG00000267914. ENST00000572663; ENSP00000459363; ENSG00000269597.
GeneID	259295.
KEGG	hsa:259295.
UCSC	uc001qzm.2. human.
Organism-specific databases
CTD	259295.
GeneCards	GC12M011148.
HGNC	HGNC:19109. TAS2R20.
MIM	613962. gene.
neXtProt	NX_P59543.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43462.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
InParanoid	P59543.
KO	K08474.
OMA	PSTKIHI.
OrthoDB	EOG42822D.
PhylomeDB	P59543.
Gene expression databases
ArrayExpress	P59543.
CleanEx	HS_TAS2R49.
Genevestigator	P59543.
Family and domain databases
InterPro	IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	259295.
NextBio	93141.
SOURCE	Search...

Entry information

Entry name

T2R20_HUMAN

Accession

Primary (citable) accession number: P59543
Secondary accession number(s): P59549 , Q2HIZ4, Q496D8, Q645X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.