Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P59543 (T2R20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 20
Alternative name(s):
Taste receptor type 2 member 49
Short name=T2R49
Taste receptor type 2 member 56
Short name=T2R56
Gene names
Name:TAS2R20
Synonyms:TAS2R49
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.

Miscellaneous

Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
   Biological processSensory transduction
Taste
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsensory perception of taste

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Taste receptor type 2 member 20
PRO_0000082331

Regions

Topological domain1 – 66Extracellular Potential
Transmembrane7 – 2721Helical; Name=1; Potential
Topological domain28 – 4619Cytoplasmic Potential
Transmembrane47 – 6721Helical; Name=2; Potential
Topological domain68 – 7912Extracellular Potential
Transmembrane80 – 10021Helical; Name=3; Potential
Topological domain101 – 12525Cytoplasmic Potential
Transmembrane126 – 14621Helical; Name=4; Potential
Topological domain147 – 17832Extracellular Potential
Transmembrane179 – 19921Helical; Name=5; Potential
Topological domain200 – 22930Cytoplasmic Potential
Transmembrane230 – 25021Helical; Name=6; Potential
Topological domain251 – 2599Extracellular Potential
Transmembrane260 – 28021Helical; Name=7; Potential
Topological domain281 – 30929Cytoplasmic Potential

Amino acid modifications

Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential

Natural variations

Natural variant791K → E. Ref.4
Corresponds to variant rs7135018 [ dbSNP | Ensembl ].
VAR_053356
Natural variant1431H → Q.
Corresponds to variant rs12226920 [ dbSNP | Ensembl ].
VAR_053357
Natural variant1481H → N. Ref.3
Corresponds to variant rs12226919 [ dbSNP | Ensembl ].
VAR_053358
Natural variant2361I → V. Ref.3
Corresponds to variant rs10845281 [ dbSNP | Ensembl ].
VAR_053359
Natural variant2521F → S. Ref.3
Corresponds to variant rs10845280 [ dbSNP | Ensembl ].
VAR_053360
Natural variant2551R → L. Ref.3
Corresponds to variant rs10845279 [ dbSNP | Ensembl ].
VAR_053361

Experimental info

Sequence conflict331I → V in AAU21140. Ref.3
Sequence conflict551R → K in AAM19327. Ref.1
Sequence conflict141 – 1433VCH → ICQ in AAU21140. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P59543 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: AB200710EE19361C

FASTA30935,358
        10         20         30         40         50         60 
MMSFLHIVFS ILVVVAFILG NFANGFIALI NFIAWVKRQK ISSADQIIAA LAVSRVGLLW 

        70         80         90        100        110        120 
VILLHWYSTV LNPTSSNLKV IIFISNAWAV TNHFSIWLAT SLSIFYLLKI VNFSRLIFHH 

       130        140        150        160        170        180 
LKRKAKSVVL VIVLGSLFFL VCHLVMKHTY INVWTEECEG NVTWKIKLRN AMHLSNLTVA 

       190        200        210        220        230        240 
MLANLIPFTL TLISFLLLIY SLCKHLKKMQ LHGKGSQDPS TKIHIKALQT VTSFLILLAI 

       250        260        270        280        290        300 
YFLCLIISFW NFKMRPKEIV LMLCQAFGII YPSFHSFILI WGNKTLKQTF LSVLWQVTCW 


AKGQNQSTP 

« Hide

References

« Hide 'large scale' references
[1]"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides."
Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.
Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification and characterization of human taste receptor genes belonging to the TAS2R family."
Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.
Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-148; VAL-236; SER-252 AND LEU-255.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-79.
[5]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF494236 Genomic DNA. Translation: AAM19327.1.
AY114092 Genomic DNA. Translation: AAM63542.1.
AY724938 Genomic DNA. Translation: AAU21140.1.
BC100915 mRNA. Translation: AAI00916.1.
BC100916 mRNA. Translation: AAI00917.1.
BC100918 mRNA. Translation: AAI00919.1.
BC113843 mRNA. Translation: AAI13844.1.
RefSeqNP_795370.2. NM_176889.2.
UniGeneHs.686384.

3D structure databases

ProteinModelPortalP59543.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000375092.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP59543.

Polymorphism databases

DMDM55977811.

Proteomic databases

PaxDbP59543.
PRIDEP59543.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000538986; ENSP00000441624; ENSG00000255837.
ENST00000570941; ENSP00000459353; ENSG00000273092.
ENST00000572663; ENSP00000459363; ENSG00000272873.
GeneID259295.
KEGGhsa:259295.
UCSCuc001qzm.2. human.

Organism-specific databases

CTD259295.
GeneCardsGC12M011148.
HGNCHGNC:19109. TAS2R20.
MIM613962. gene.
neXtProtNX_P59543.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43462.
HOGENOMHOG000234595.
HOVERGENHBG060698.
InParanoidP59543.
KOK08474.
OMAPSTKIHI.
OrthoDBEOG7P02JB.
PhylomeDBP59543.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP59543.
CleanExHS_TAS2R49.
GenevestigatorP59543.

Family and domain databases

InterProIPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R20.
GenomeRNAi259295.
NextBio93141.
PROP59543.
SOURCESearch...

Entry information

Entry nameT2R20_HUMAN
AccessionPrimary (citable) accession number: P59543
Secondary accession number(s): P59549 expand/collapse secondary AC list , Q2HIZ4, Q496D8, Q645X9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: November 23, 2004
Last modified: February 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries