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P59540 (T2R46_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 46

Short name=T2R46
Alternative name(s):
Taste receptor type 2 member 54
Short name=T2R54
Gene names
Name:TAS2R46
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity. In airway epithelial cells, binding of bitter compounds increases the intracellular calcium ion concentration and stimulates ciliary beat frequency By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Cell projectioncilium membrane. Note: In airway epithelial cells, localizes to motile cilia. Ref.7

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Expressed on ciliated airway epithelium. Ref.7

Miscellaneous

Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Taste receptor type 2 member 46
PRO_0000082318

Regions

Topological domain11Extracellular Potential
Transmembrane2 – 2221Helical; Name=1; Potential
Topological domain23 – 4624Cytoplasmic Potential
Transmembrane47 – 6721Helical; Name=2; Potential
Topological domain68 – 8619Extracellular Potential
Transmembrane87 – 10721Helical; Name=3; Potential
Topological domain108 – 12619Cytoplasmic Potential
Transmembrane127 – 14721Helical; Name=4; Potential
Topological domain148 – 17831Extracellular Potential
Transmembrane179 – 19921Helical; Name=5; Potential
Topological domain200 – 22930Cytoplasmic Potential
Transmembrane230 – 25021Helical; Name=6; Potential
Topological domain251 – 2599Extracellular Potential
Transmembrane260 – 28021Helical; Name=7; Potential
Topological domain281 – 30929Cytoplasmic Potential

Amino acid modifications

Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential

Natural variations

Natural variant2281L → M. Ref.2
Corresponds to variant rs2708380 [ dbSNP | Ensembl ].
VAR_062091

Experimental info

Sequence conflict296 – 2972HV → QM Ref.3

Sequences

Sequence LengthMass (Da)Tools
P59540 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: FF4D7747F0CE47EF

FASTA30935,523
        10         20         30         40         50         60 
MITFLPIIFS ILIVVTFVIG NFANGFIALV NSIEWFKRQK ISFADQILTA LAVSRVGLLW 

        70         80         90        100        110        120 
VLVLNWYATE LNPAFNSIEV RITAYNVWAV INHFSNWLAT SLSIFYLLKI ANFSNLIFLH 

       130        140        150        160        170        180 
LKRRVKSVVL VILLGPLLFL VCHLFVINMN QIIWTKEYEG NMTWKIKLRS AMYLSNTTVT 

       190        200        210        220        230        240 
ILANLVPFTL TLISFLLLIC SLCKHLKKMQ LHGKGSQDPS MKVHIKALQT VTSFLLLCAI 

       250        260        270        280        290        300 
YFLSIIMSVW SFESLENKPV FMFCEAIAFS YPSTHPFILI WGNKKLKQTF LSVLWHVRYW 


VKGEKPSSS 

« Hide

References

[1]"Identification and characterization of human taste receptor genes belonging to the TAS2R family."
Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.
Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT MET-228.
[3]"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides."
Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.
Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-299.
[4]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[5]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Motile cilia of human airway epithelia are chemosensory."
Shah A.S., Ben-Shahar Y., Moninger T.O., Kline J.N., Welsh M.J.
Science 325:1131-1134(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Web resources

Protein Spotlight

A tail of protection - Issue 119 of July 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY114091 Genomic DNA. Translation: AAM63541.1.
AY724941 Genomic DNA. Translation: AAU21143.1.
AF494227 Genomic DNA. Translation: AAM19318.1.
CCDSCCDS53748.1.
RefSeqNP_795368.2. NM_176887.2.
UniGeneHs.688193.

3D structure databases

ProteinModelPortalP59540.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP59540.

Polymorphism databases

DMDM55977809.

Proteomic databases

PRIDEP59540.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000533467; ENSP00000436450; ENSG00000226761.
ENST00000574263; ENSP00000460229; ENSG00000262525.
GeneID259292.
KEGGhsa:259292.
UCSCuc001qzp.1. human.

Organism-specific databases

CTD259292.
GeneCardsGC12M011214.
HGNCHGNC:18877. TAS2R46.
MIM612774. gene.
neXtProtNX_P59540.
PharmGKBPA38731.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG060698.
KOK08474.
OMAFMFCEAI.
OrthoDBEOG7P02JB.
PhylomeDBP59540.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP59540.
CleanExHS_TAS2R46.
GenevestigatorP59540.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR017452. GPCR_Rhodpsn_7TM.
IPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R46.
GenomeRNAi259292.
NextBio93127.
PROP59540.
SOURCESearch...

Entry information

Entry nameT2R46_HUMAN
AccessionPrimary (citable) accession number: P59540
Secondary accession number(s): P59548, Q645X6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: November 23, 2004
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries