P59537 (T2R43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 43
Short name=T2R43

Alternative name(s):
Taste receptor type 2 member 52
Short name=T2R52

Gene names

Name:

TAS2R43

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Gustducin-coupled receptor immplicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. Activated by the sulfonyl amide sweeteners saccharin and acesulfame K. In airway epithelial cells, binding of bitter compounds increases the intracellular calcium ion concentration and stimulates ciliary beat frequency. May act as chemosensory receptors in airway epithelial cells to detect and eliminate potential noxious agents from the airways By similarity.
Subcellular location	Membrane; Multi-pass membrane protein. Cell projection › cilium membrane. Note: In airway epithelial cells, localizes to motile cilia. Ref.10
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Expressed in airway epithelia. Ref.10
Miscellaneous	Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Cell membrane Cell projection Cilium Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cilium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred by curator PubMed 16720576. Source: HGNC motile cilium Inferred from direct assay Ref.10. Source: UniProtKB
Molecular_function	bitter taste receptor activity Inferred from direct assay PubMed 16720576. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Taste receptor type 2 member 43		PRO_0000082302
Regions
	Topological domain	1	1	Extracellular Potential
	Transmembrane	2 – 22	21	Helical; Name=1; Potential
	Topological domain	23 – 46	24	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Helical; Name=2; Potential
	Topological domain	68 – 86	19	Extracellular Potential
	Transmembrane	87 – 107	21	Helical; Name=3; Potential
	Topological domain	108 – 126	19	Cytoplasmic Potential
	Transmembrane	127 – 147	21	Helical; Name=4; Potential
	Topological domain	148 – 178	31	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Name=5; Potential
	Topological domain	200 – 229	30	Cytoplasmic Potential
	Transmembrane	230 – 250	21	Helical; Name=6; Potential
	Topological domain	251 – 259	9	Extracellular Potential
	Transmembrane	260 – 280	21	Helical; Name=7; Potential
	Topological domain	281 – 309	29	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	35	1	W → S in AAM19328. Ref.1
	Sequence conflict	35	1	W → S in AAU21145. Ref.3
	Sequence conflict	35	1	W → S in AAI17424. Ref.5
	Sequence conflict	212	1	H → R in AAM19328. Ref.1
	Sequence conflict	212	1	H → R in AAU21145. Ref.3
	Sequence conflict	212	1	H → R in AAI17424. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P59537 [UniParc]. Last modified January 25, 2012. Version 2. Checksum: 3B4FCD1089EB6857	FASTA	309	35,599
	10 20 30 40 50 60 MITFLPIIFS SLVVVTFVIG NFANGFIALV NSIEWFKRQK ISFADQILTA LAVSRVGLLW 70 80 90 100 110 120 VLLLNWYSTV LNPAFNSVEV RTTAYNIWAV INHFSNWLAT TLSIFYLLKI ANFSNFIFLH 130 140 150 160 170 180 LKRRVKSVIL VMLLGPLLFL ACHLFVINMN EIVRTKEFEG NMTWKIKLKS AMYFSNMTVT 190 200 210 220 230 240 MVANLVPFTL TLLSFMLLIC SLCKHLKKMQ LHGKGSQDPS TKVHIKALQT VISFLLLCAI 250 260 270 280 290 300 YFLSIMISVW SFGSLENKPV FMFCKAIRFS YPSIHPFILI WGNKKLKQTF LSVFWQMRYW VKGEKTSSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W. Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification and characterization of human taste receptor genes belonging to the TAS2R family." Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J. Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[8]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"Bitter taste receptors for saccharin and acesulfame K." Kuhn C., Bufe B., Winnig M., Hofmann T., Frank O., Behrens M., Lewtschenko T., Slack J.P., Ward C.D., Meyerhof W. J. Neurosci. 24:10260-10265(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVATION BY SACCHARIN AND ACESULFAME K.
[10]	"Motile cilia of human airway epithelia are chemosensory." Shah A.S., Ben-Shahar Y., Moninger T.O., Kline J.N., Welsh M.J. Science 325:1131-1134(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

A tail of protection - Issue 119 of July 2010

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF494237 Genomic DNA. Translation: AAM19328.1. AY114089 Genomic DNA. Translation: AAM63539.1. AY724943 Genomic DNA. Translation: AAU21145.1. AC018630 Genomic DNA. No translation available. BC117423 mRNA. Translation: AAI17424.1.
IPI	IPI00259669.
RefSeq	NP_795365.2. NM_176884.2.
UniGene	Hs.688195.
3D structure databases
ProteinModelPortal	P59537.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	29839475.
Proteomic databases
PaxDb	P59537.
PRIDE	P59537.
Protocols and materials databases
DNASU	259289.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000531678; ENSP00000431719; ENSG00000255374. ENST00000571879; ENSP00000458723; ENSG00000262612.
GeneID	259289.
KEGG	hsa:259289.
Organism-specific databases
CTD	259289.
GeneCards	GC12M011243.
HGNC	HGNC:18875. TAS2R43.
MIM	612668. gene.
neXtProt	NX_P59537.
PharmGKB	PA38729.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43462.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
KO	K08474.
OMA	LRTTAYN.
OrthoDB	EOG42822D.
PhylomeDB	P59537.
Gene expression databases
ArrayExpress	P59537.
CleanEx	HS_TAS2R43.
Genevestigator	P59537.
Family and domain databases
InterPro	IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	259289.
NextBio	93115.
SOURCE	Search...

Entry information

Entry name

T2R43_HUMAN

Accession

Primary (citable) accession number: P59537
Secondary accession number(s): P59546, Q645X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	January 25, 2012
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.