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P59537 (T2R43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 43

Short name=T2R43
Alternative name(s):
Taste receptor type 2 member 52
Short name=T2R52
Gene names
Name:TAS2R43
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Gustducin-coupled receptor immplicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. Activated by the sulfonyl amide sweeteners saccharin and acesulfame K. In airway epithelial cells, binding of bitter compounds increases the intracellular calcium ion concentration and stimulates ciliary beat frequency. May act as chemosensory receptors in airway epithelial cells to detect and eliminate potential noxious agents from the airways By similarity.

Subcellular location

Membrane; Multi-pass membrane protein. Cell projectioncilium membrane. Note: In airway epithelial cells, localizes to motile cilia. Ref.10

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. Expressed in airway epithelia. Ref.10

Miscellaneous

Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Taste receptor type 2 member 43
PRO_0000082302

Regions

Topological domain11Extracellular Potential
Transmembrane2 – 2221Helical; Name=1; Potential
Topological domain23 – 4624Cytoplasmic Potential
Transmembrane47 – 6721Helical; Name=2; Potential
Topological domain68 – 8619Extracellular Potential
Transmembrane87 – 10721Helical; Name=3; Potential
Topological domain108 – 12619Cytoplasmic Potential
Transmembrane127 – 14721Helical; Name=4; Potential
Topological domain148 – 17831Extracellular Potential
Transmembrane179 – 19921Helical; Name=5; Potential
Topological domain200 – 22930Cytoplasmic Potential
Transmembrane230 – 25021Helical; Name=6; Potential
Topological domain251 – 2599Extracellular Potential
Transmembrane260 – 28021Helical; Name=7; Potential
Topological domain281 – 30929Cytoplasmic Potential

Amino acid modifications

Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict351W → S in AAM19328. Ref.1
Sequence conflict351W → S in AAU21145. Ref.3
Sequence conflict351W → S in AAI17424. Ref.5
Sequence conflict2121H → R in AAM19328. Ref.1
Sequence conflict2121H → R in AAU21145. Ref.3
Sequence conflict2121H → R in AAI17424. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P59537 [UniParc].

Last modified January 25, 2012. Version 2.
Checksum: 3B4FCD1089EB6857

FASTA30935,599
        10         20         30         40         50         60 
MITFLPIIFS SLVVVTFVIG NFANGFIALV NSIEWFKRQK ISFADQILTA LAVSRVGLLW 

        70         80         90        100        110        120 
VLLLNWYSTV LNPAFNSVEV RTTAYNIWAV INHFSNWLAT TLSIFYLLKI ANFSNFIFLH 

       130        140        150        160        170        180 
LKRRVKSVIL VMLLGPLLFL ACHLFVINMN EIVRTKEFEG NMTWKIKLKS AMYFSNMTVT 

       190        200        210        220        230        240 
MVANLVPFTL TLLSFMLLIC SLCKHLKKMQ LHGKGSQDPS TKVHIKALQT VISFLLLCAI 

       250        260        270        280        290        300 
YFLSIMISVW SFGSLENKPV FMFCKAIRFS YPSIHPFILI WGNKKLKQTF LSVFWQMRYW 


VKGEKTSSP 

« Hide

References

« Hide 'large scale' references
[1]"The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides."
Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.
Nat. Genet. 32:397-401(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification and characterization of human taste receptor genes belonging to the TAS2R family."
Conte C., Ebeling M., Marcuz A., Nef P., Andres-Barquin P.J.
Cytogenet. Genome Res. 98:45-53(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Bitter taste receptors for saccharin and acesulfame K."
Kuhn C., Bufe B., Winnig M., Hofmann T., Frank O., Behrens M., Lewtschenko T., Slack J.P., Ward C.D., Meyerhof W.
J. Neurosci. 24:10260-10265(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIVATION BY SACCHARIN AND ACESULFAME K.
[10]"Motile cilia of human airway epithelia are chemosensory."
Shah A.S., Ben-Shahar Y., Moninger T.O., Kline J.N., Welsh M.J.
Science 325:1131-1134(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

A tail of protection - Issue 119 of July 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF494237 Genomic DNA. Translation: AAM19328.1.
AY114089 Genomic DNA. Translation: AAM63539.1.
AY724943 Genomic DNA. Translation: AAU21145.1.
AC018630 Genomic DNA. No translation available.
BC117423 mRNA. Translation: AAI17424.1.
CCDSCCDS53749.1.
RefSeqNP_795365.2. NM_176884.2.
UniGeneHs.688195.

3D structure databases

ProteinModelPortalP59537.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM374095447.

Proteomic databases

PaxDbP59537.
PRIDEP59537.

Protocols and materials databases

DNASU259289.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000531678; ENSP00000431719; ENSG00000255374.
ENST00000571879; ENSP00000458723; ENSG00000262612.
GeneID259289.
KEGGhsa:259289.
UCSCuc001qzq.1. human.

Organism-specific databases

CTD259289.
GeneCardsGC12M011243.
HGNCHGNC:18875. TAS2R43.
MIM612668. gene.
neXtProtNX_P59537.
PharmGKBPA38729.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43462.
HOGENOMHOG000234595.
HOVERGENHBG060698.
KOK08474.
OMAKNLENKP.
OrthoDBEOG7P02JB.
PhylomeDBP59537.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP59537.
CleanExHS_TAS2R43.
GenevestigatorP59537.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR017452. GPCR_Rhodpsn_7TM.
IPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R43.
GenomeRNAi259289.
NextBio93115.
PROP59537.
SOURCESearch...

Entry information

Entry nameT2R43_HUMAN
AccessionPrimary (citable) accession number: P59537
Secondary accession number(s): P59546, Q645X4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: January 25, 2012
Last modified: July 9, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries