P59517 (SYN_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	bbp_329

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176399

Sequences

	Sequence		Length	Mass (Da)
	P59517 [UniParc]. Last modified April 11, 2003. Version 1. Checksum: 200C35A0BECC9DFE	FASTA	466	54,108
	10 20 30 40 50 60 MKTVSIFDIY LNKIKLNSKI NIQGWVRNRR RSKLGLLFID LYDGSCLYTM QVVVKDILSN 70 80 90 100 110 120 YDSEISKLSV GCSINVFGVL ISSIGTKQKY EIQAKVVKVI GIINNPGSYP ISSKYHTMKH 130 140 150 160 170 180 LRNVPHLRSR TNFFGAVSRV RNYLFNALHE FLFKNGYYWI PSPIITGLNS EGAGDMFKVS 190 200 210 220 230 240 ILDLNNIPKN KNGKINFKKD FFGKEAFLTV SGQLTLETYA CSLSKVYSFG PIFRAENSNT 250 260 270 280 290 300 RRHLSEFWML EVETAFSNLC DIIKFSENML KYAIGIILDK CVIDINFFRE NVDEKIFCRL 310 320 330 340 350 360 KDFSSKQFFQ IEYKEAINIL IKSNRFDHKV MFIGMELSSD HERFLVEEYF KFPLVITNYP 370 380 390 400 410 420 KSLKAFYMRV NDDNKTVSAM DVLVPGVGEI IGGSEREERL NVLDRRFFEL NLNKEDYWWY 430 440 450 460 RDLRKYGTIP HSGFGLGFER LLSYILGIKN IRDVCPFPRT AYKADF

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27050.1.
RefSeq	NP_777945.1. NC_004545.1.
3D structure databases
ProteinModelPortal	P59517.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002562; EBBUCP00000002386; EBBUCG00000002562.
GeneID	1058271.
GenomeReviews	Gene locus bbp_329 in contig AE016826_GR.
KEGG	bab:bbp329.
PATRIC	21245447. VBIBucAph80364_0323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007970.
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	P59517.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_329-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BUCBP

Accession

Primary (citable) accession number: P59517

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	April 11, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program