Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

A disintegrin and metalloproteinase with thrombospondin motifs 20

Gene

Adamts20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in tissue-remodeling process occurring in both normal and pathological conditions. May have a protease-independent function in the transport from the endoplasmic reticulum to the Golgi apparatus of secretory cargos, mediated by the GON domain.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi399 – 3991Zinc; catalyticBy similarity
Active sitei400 – 4001PROSITE-ProRule annotation
Metal bindingi403 – 4031Zinc; catalyticBy similarity
Metal bindingi409 – 4091Zinc; catalyticBy similarity

GO - Molecular functioni

  • endopeptidase activity Source: MGI
  • metalloendopeptidase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • extracellular matrix organization Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • positive regulation of melanocyte differentiation Source: MGI
  • positive regulation of signal transduction Source: MGI
  • proteolysis Source: MGI
  • regulation of developmental pigmentation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.188.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 20 (EC:3.4.24.-)
Short name:
ADAM-TS 20
Short name:
ADAM-TS20
Short name:
ADAMTS-20
Gene namesi
Name:Adamts20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2660628. Adamts20.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Adamts20 are the cause of the belted (bt) phenotype. It is a pigmental defect which occurs as a result of a defect in melanocyte development.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Propeptidei27 – 249223By similarityPRO_0000029208Add
BLAST
Chaini250 – 19061657A disintegrin and metalloproteinase with thrombospondin motifs 20PRO_0000029209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence analysis
Disulfide bondi330 ↔ 383By similarity
Disulfide bondi359 ↔ 365By similarity
Disulfide bondi377 ↔ 459By similarity
Disulfide bondi415 ↔ 443By similarity
Disulfide bondi486 ↔ 508By similarity
Disulfide bondi497 ↔ 518By similarity
Disulfide bondi503 ↔ 537By similarity
Disulfide bondi531 ↔ 542By similarity
Disulfide bondi565 ↔ 602By similarity
Disulfide bondi569 ↔ 607By similarity
Disulfide bondi580 ↔ 592By similarity
Glycosylationi714 – 7141N-linked (GlcNAc...)Sequence analysis
Glycosylationi798 – 7981N-linked (GlcNAc...)Sequence analysis
Glycosylationi805 – 8051N-linked (GlcNAc...)Sequence analysis
Glycosylationi1057 – 10571N-linked (GlcNAc...)Sequence analysis
Glycosylationi1562 – 15621N-linked (GlcNAc...)Sequence analysis
Glycosylationi1719 – 17191N-linked (GlcNAc...)Sequence analysis
Glycosylationi1759 – 17591N-linked (GlcNAc...)Sequence analysis
Glycosylationi1777 – 17771N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP59511.
PRIDEiP59511.

PTM databases

PhosphoSiteiP59511.

Expressioni

Tissue specificityi

Expressed at low level in testis and brain.

Gene expression databases

BgeeiP59511.
GenevisibleiP59511. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036330.

Structurei

3D structure databases

ProteinModelPortaliP59511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini255 – 464210Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini465 – 55288DisintegrinAdd
BLAST
Domaini553 – 60856TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini843 – 90159TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini906 – 96257TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini962 – 101554TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini1017 – 107458TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini1075 – 113157TSP type-1 6PROSITE-ProRule annotationAdd
BLAST
Domaini1148 – 120255TSP type-1 7PROSITE-ProRule annotationAdd
BLAST
Domaini1203 – 126058TSP type-1 8PROSITE-ProRule annotationAdd
BLAST
Domaini1300 – 135152TSP type-1 9PROSITE-ProRule annotationAdd
BLAST
Domaini1354 – 141158TSP type-1 10PROSITE-ProRule annotationAdd
BLAST
Domaini1412 – 146554TSP type-1 11PROSITE-ProRule annotationAdd
BLAST
Domaini1468 – 152659TSP type-1 12PROSITE-ProRule annotationAdd
BLAST
Domaini1527 – 158458TSP type-1 13PROSITE-ProRule annotationAdd
BLAST
Domaini1585 – 164864TSP type-1 14PROSITE-ProRule annotationAdd
BLAST
Domaini1650 – 170657TSP type-1 15PROSITE-ProRule annotationAdd
BLAST
Domaini1707 – 1906200GONPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni721 – 842122SpacerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi609 – 720112Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 disintegrin domain.Curated
Contains 1 GON domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
Contains 15 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
ENOG410XPKZ. LUCA.
GeneTreeiENSGT00760000118880.
HOGENOMiHOG000004798.
HOVERGENiHBG037334.
InParanoidiP59511.
KOiK09609.
OMAiCGPGMHC.
OrthoDBiEOG7WDN1M.
TreeFamiTF331949.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR012314. Pept_M12B_GON-ADAMTSs.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF08685. GON. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 11 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 14 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 13 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS51046. GON. 1 hit.
PS50092. TSP1. 13 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P59511-1) [UniParc]FASTAAdd to basket

Also known as: ADAMTS20 B long isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVAKWLTGL LCPISLLLTG SWEVRFHPRQ EALVKTLASY EVVTPTRVNE
60 70 80 90 100
FGDVFPQNRH FSRKKRSSGV PEPPPFRTHY RISAYGQLFQ LNLSADAAFL
110 120 130 140 150
AAGYTEVHLG TPVPGPGGRS TESPDLRHCF YRGQVNARED HTAVFSLCGG
160 170 180 190 200
LMGTFKANDG EYFLEPVLRA DGSAHDDDHN KPHLIYRQEL KRNSFARSHK
210 220 230 240 250
PCEVSENQME KTALPSQSSR NTTGDVDIEE EAVFRLEGER SQLHSRNKRF
260 270 280 290 300
LSYPRYVEVM VTADAKMVHH HGQNLQHYVL TLMSIVAAIY KDSSIGNLIN
310 320 330 340 350
IVIVKLVVIH SEQEGPVISF NAATTLRNFC LWQQSQNVPD DAHPSHHDTA
360 370 380 390 400
VLITREDICG AKEKCDTLGL AELGTLCDPS RSCSISEENG LSAAFTIAHE
410 420 430 440 450
LGHVFNVPHD DSFKCKEAGI KHQYHVMAPT LNYHTSPWTW SACSQKHITE
460 470 480 490 500
FLDTGHGECL LDKPNGRTYD LSPQLPGSVY DGNRQCELMF GPGSQVCPYL
510 520 530 540 550
KHCRRLWCTS AEGVHKGCRT QHMPLADGTS CGPGMHCHRG LCVTRDMETR
560 570 580 590 600
PVDGEWGPWG PYSSCSRTCG GGIKSTARLC DRPEPRNGGR YCVGRRMKFR
610 620 630 640 650
SCNTDSCPKG KRDFREKQCS DFDGKHFDIN GLPPNVRWLP KYSGIAVKDR
660 670 680 690 700
CKLYCRVAGT TSFYQLKDRV ADGTPCGTET NDICVQGLCR QAGCDHVLNS
710 720 730 740 750
KAKRDKCGVC GGDNSSCQTL AGVFNSAHYG YNVVVKIPAG ATNIEILQHS
760 770 780 790 800
YSGRPEDDNY LALSDTQGNF LLNGNFVVSM AKKEINIQGA VFEYSGSNNS
810 820 830 840 850
IERINSTDRL EAELVLQVLC VGNLYNPDVR YSFNIPIEER SNLFSWDPYG
860 870 880 890 900
PWQDCTKMCQ GLHRRKIACV RKSDHAVVSD HNCGHLPMPL FVTEKCNMDC
910 920 930 940 950
ELRWHIIGKS DCSSQCGQGY RTLDVHCMKY SVHKGQAVPV GDQYCGDQLK
960 970 980 990 1000
PPSREPCHGS CVLTRWHYSE WSQCSRSCGG GDKTRESYCV NGFGHRLAES
1010 1020 1030 1040 1050
ECRELPRVVL ENCNEFPCPS WATSEWSECP VTCGKGMKQR QVWCQLSEDP
1060 1070 1080 1090 1100
MRDGFCNAST KPESLRPCEL RACASWHVGP WGSCTATCGH GYQMRAVKCI
1110 1120 1130 1140 1150
SEIFGTMLDD RECPQASRPS DRQDCILAPC LAIPEVGATS LPAIPLGRAA
1160 1170 1180 1190 1200
QWRHGSWTPC SVSCGRGSQA RYVSCRDAHD EVADESNCAH LPRPAAVSLC
1210 1220 1230 1240 1250
FSPCGEWQAG DWSPCSASCG HGKTTRRVLC VNYHQLVDES YCDPEGRPVT
1260 1270 1280 1290 1300
EQECSLAACP PLYSRAPSSS EQPSHVPSRN VPLTHKPGEN QDQGAQLSIR
1310 1320 1330 1340 1350
GNQWRTGPWG ACSRSCAGGL QHRAVVCQDE DGRSATSCDG SSKPPESRHC
1360 1370 1380 1390 1400
GSGPCPHWNY GDWGECTQTC GGGVKSRFVI CQFPNGQMTQ EHSCELPKPP
1410 1420 1430 1440 1450
SMMQCHLHAC PEDVSWYRGP WKSCSASCGK GVKYREVLCI DQFQRKLEEK
1460 1470 1480 1490 1500
YCSHLHKPRT HKACRSGRCP SWKANKWKEC SVTCGSGVQQ REVYCRLRGT
1510 1520 1530 1540 1550
GRVSEDMCDP STRPQGQRQC WRQDCMRYQW TTGDWLDCST SCKKKETYRL
1560 1570 1580 1590 1600
VKCVNEQNVQ ANESLCDPLT KPLSIKKCRN PHCKYSVVTG DSSQCAGNCG
1610 1620 1630 1640 1650
FTSPQKITYC TKIQSSKKHT FHQLRPVVYG ECPVIPSPQA YKCDLRSCLH
1660 1670 1680 1690 1700
VATWKVGKWS KCSVTCGIGI MERRVACRTE NGWPSDLCLK RLKPDAQKKC
1710 1720 1730 1740 1750
YANDCKLLTT CKELQVTNNV TKDGDYDLNV RGRILKIHCS GMQLENPREY
1760 1770 1780 1790 1800
LPLVKSEDNF SEIYGLRLQN PYECPFNGSR RPDCACENDY LPAGYTVFSK
1810 1820 1830 1840 1850
VRVDLESMQI KTADLLFSQT LSGKAVPFAT AGDCYSAARC PQGQFSINLA
1860 1870 1880 1890 1900
GTGMKISNTA KWLAQGRYAS VIIHRSQDGT KVYGRCGGFC GKCIPHMATG

LSIQVL
Length:1,906
Mass (Da):212,068
Last modified:July 27, 2011 - v2
Checksum:iA7B152DA0D93CD03
GO
Isoform 2 (identifier: P59511-2) [UniParc]FASTAAdd to basket

Also known as: ADAMTS20 A short isoform

The sequence of this isoform differs from the canonical sequence as follows:
     1424-1425: CS → VR
     1426-1906: Missing.

Show »
Length:1,425
Mass (Da):158,027
Checksum:iACBF24456E3EAFD2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1211 – 12111D → Y in AAO74895 (PubMed:12925592).Curated
Sequence conflicti1211 – 12111D → Y in AAO74896 (PubMed:12925592).Curated
Sequence conflicti1262 – 12621L → S in CAD54808 (PubMed:12562771).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1424 – 14252CS → VR in isoform 2. 1 PublicationVSP_007606
Alternative sequencei1426 – 1906481Missing in isoform 2. 1 PublicationVSP_007607Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512753 mRNA. Translation: CAD54808.3.
AY189815 mRNA. Translation: AAO74895.1.
AY189816 mRNA. Translation: AAO74896.1.
AC084382 Genomic DNA. No translation available.
AC084384 Genomic DNA. No translation available.
CCDSiCCDS27770.1. [P59511-1]
CCDS49711.1. [P59511-2]
RefSeqiNP_803180.3. NM_177431.4. [P59511-1]
UniGeneiMm.214132.

Genome annotation databases

EnsembliENSMUST00000035342; ENSMUSP00000036330; ENSMUSG00000022449. [P59511-1]
ENSMUST00000155907; ENSMUSP00000121696; ENSMUSG00000022449. [P59511-2]
GeneIDi223838.
KEGGimmu:223838.
UCSCiuc007xje.2. mouse. [P59511-1]
uc007xjf.2. mouse. [P59511-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512753 mRNA. Translation: CAD54808.3.
AY189815 mRNA. Translation: AAO74895.1.
AY189816 mRNA. Translation: AAO74896.1.
AC084382 Genomic DNA. No translation available.
AC084384 Genomic DNA. No translation available.
CCDSiCCDS27770.1. [P59511-1]
CCDS49711.1. [P59511-2]
RefSeqiNP_803180.3. NM_177431.4. [P59511-1]
UniGeneiMm.214132.

3D structure databases

ProteinModelPortaliP59511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036330.

Protein family/group databases

MEROPSiM12.188.

PTM databases

PhosphoSiteiP59511.

Proteomic databases

PaxDbiP59511.
PRIDEiP59511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035342; ENSMUSP00000036330; ENSMUSG00000022449. [P59511-1]
ENSMUST00000155907; ENSMUSP00000121696; ENSMUSG00000022449. [P59511-2]
GeneIDi223838.
KEGGimmu:223838.
UCSCiuc007xje.2. mouse. [P59511-1]
uc007xjf.2. mouse. [P59511-2]

Organism-specific databases

CTDi80070.
MGIiMGI:2660628. Adamts20.

Phylogenomic databases

eggNOGiKOG3538. Eukaryota.
ENOG410XPKZ. LUCA.
GeneTreeiENSGT00760000118880.
HOGENOMiHOG000004798.
HOVERGENiHBG037334.
InParanoidiP59511.
KOiK09609.
OMAiCGPGMHC.
OrthoDBiEOG7WDN1M.
TreeFamiTF331949.

Miscellaneous databases

ChiTaRSiAdamts20. mouse.
NextBioi376929.
PROiP59511.
SOURCEiSearch...

Gene expression databases

BgeeiP59511.
GenevisibleiP59511. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR012314. Pept_M12B_GON-ADAMTSs.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF08685. GON. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 11 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 14 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 13 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS51046. GON. 1 hit.
PS50092. TSP1. 13 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of ADAMTS-20 defines a novel subfamily of metalloproteinases-disintegrins with multiple thrombospondin-1 repeats and a unique GON domain."
    Llamazares M., Cal S., Quesada V., Lopez-Otin C.
    J. Biol. Chem. 278:13382-13389(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  2. "A defect in a novel ADAMTS family member is the cause of the belted white-spotting mutation."
    Rao C., Foernzler D., Loftus S.K., Liu S., McPherson J.D., Jungers K.A., Apte S.S., Pavan W.J., Beier D.R.
    Development 130:4665-4672(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), DISEASE.
    Strain: DBA/2J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiATS20_MOUSE
AccessioniPrimary (citable) accession number: P59511
Secondary accession number(s): E9QLA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.