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Protein

Glutathione synthetase

Gene

gshB

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi283Magnesium or manganeseUniRule annotation1
Metal bindingi285Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 209ATPUniRule annotationAdd BLAST58

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH224915:G1G09-497-MONOMER
UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:bbp_490
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000601 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001974611 – 324Glutathione synthetaseAdd BLAST324

Proteomic databases

PRIDEiP59495

Interactioni

Protein-protein interaction databases

STRINGi224915.bbp490

Structurei

3D structure databases

ProteinModelPortaliP59495
SMRiP59495
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 312ATP-graspUniRule annotationAdd BLAST188

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z Bacteria
COG0189 LUCA
KOiK01920
OMAiWMRKDPP
OrthoDBiPOG091H04J6

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

P59495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTINLGIIMD PISSINIKKD SSFAILLEAQ NRKYKIYYME LKDLYLKDNK
60 70 80 90 100
PYSHTKLLRI KNNKKQWFTL EQQKDVSLSN LDVILMRKNP PINRAYIYAT
110 120 130 140 150
YILEQAERNG SYIINKPSSL RSYNEKLFTT THFPQYIPKT LITSNSTKIH
160 170 180 190 200
NFIKTYKDII IKPLHGMAGL SIFRIKEHDP NTSVIIETMT KYETIPCISQ
210 220 230 240 250
NYITDIQKGD KRILIINGIP FPWCLARIPK KHENRGNLSI GGYGNTQKLS
260 270 280 290 300
KNDWEIALSI APTLNKKGIF FAGIDIIGTK LTEINITSPT CIQEIEQDTG
310 320
ISISTIILDN LEKNLKKRKT NRYL
Length:324
Mass (Da):37,363
Last modified:April 4, 2003 - v1
Checksum:i95B1F0E16987167B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA Translation: AAO27195.1
RefSeqiWP_011091596.1, NC_004545.1

Genome annotation databases

EnsemblBacteriaiAAO27195; AAO27195; bbp_490
KEGGibab:bbp_490

Similar proteinsi

Entry informationi

Entry nameiGSHB_BUCBP
AccessioniPrimary (citable) accession number: P59495
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: April 4, 2003
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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