Reviewed, UniProtKB/Swiss-Prot P59488 (AROK_BUCBP)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	bbp_481

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Shikimate kinase HAMAP MF_00109		PRO_0000192371
Regions
	Nucleotide binding	14 – 19	6	ATP By similarity
Sites
	Metal binding	18	1	Magnesium By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	82	1	Substrate; via amide nitrogen By similarity
	Binding site	120	1	ATP By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	158	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59488-1 [UniParc]. Last modified April 4, 2003. Version 1. Checksum: 835B5E70D28054B6	FASTA	174	19,984
	10 20 30 40 50 60 MTEKRNIFLI GPMGAGKSTI GRHLAQQLSM EFYDSDQEIE KRTGVDVSWI FDIEGELGFR 70 80 90 100 110 120 KREQKIISEI IDKRGIVLST GGGSILSREI RNKLSSRGVV IYLETSVEKQ LVRTKKNKQR 130 140 150 160 170 PLLQVDNTSI QTVLEELAFH RNPLYQSIAD ITIRMNDRSI KAIVFYIIRL LNNF

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27187.1.
RefSeq	NP_778082.1.
3D structure databases
HSSP	HSSP built from PDB template 1L4U based on UniProtKB P95014.
ModBase	Search...
Genome annotation databases
GeneID	1058337.
GenomeReviews	Gene locus bbp_481 in contig AE016826_GR.
KEGG	bab:bbp481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59488.
OMA	HANVFFV.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_481-MON.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BUCBP

Accession

Primary (citable) accession number: P59488

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	April 4, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)