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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

Polr3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1080ZincBy similarity1
Metal bindingi1083ZincBy similarity1
Metal bindingi1092ZincBy similarity1
Metal bindingi1095ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 127.6 kDa polypeptide
DNA-directed RNA polymerase III subunit B
Gene namesi
Name:Polr3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1917678. Polr3b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480931 – 1133DNA-directed RNA polymerase III subunit RPC2Add BLAST1133

Proteomic databases

EPDiP59470.
MaxQBiP59470.
PaxDbiP59470.
PeptideAtlasiP59470.
PRIDEiP59470.

PTM databases

iPTMnetiP59470.
PhosphoSitePlusiP59470.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034453.
CleanExiMM_POLR3B.
GenevisibleiP59470. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076418.

Structurei

3D structure databases

ProteinModelPortaliP59470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0215. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00860000133818.
HOGENOMiHOG000222962.
HOVERGENiHBG017744.
InParanoidiP59470.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG091G00PZ.
PhylomeDBiP59470.
TreeFamiTF103047.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVLAEEFGS LTPEQLTAPI PTVEEKWRLL PAFLKVKGLV KQHIDSFNYF
60 70 80 90 100
INVEIKKIMK ANEKVTSDAD PMWYLKYLNI YVGLPDVEES FNVTRPVSPH
110 120 130 140 150
ECRLRDMTYS APITVDIEYT RGSQRIIRNA LPIGRMPIML RSSNCVLTGK
160 170 180 190 200
TPAEFAKLNE CPLDPGGYFI VKGVEKVILI QEQLSKNRII VEADRKGAVG
210 220 230 240 250
ASVTSSTHEK KSRTNMAVKQ GRFYLRHNTL SEDIPIVIIF KAMGVESDQE
260 270 280 290 300
IVQMIGTEEH VMAAFGPSLE ECQKAQIFTQ MQALKYIGNK VRRQRMWGGG
310 320 330 340 350
PKKTKIEEAR ELLASTILTH VPVKEFNFRA KCIYTAVMVR RVILAQGDNK
360 370 380 390 400
VDDRDYYGNK RLELAGQLLS LLFEDLFKKF NSEMKKIADQ VIPKQRAAQF
410 420 430 440 450
DVVKHMRQDQ ITNGMVNAIS TGNWSLKRFK MDRQGVTQVL SRLSYISALG
460 470 480 490 500
MMTRISSQFE KTRKVSGPRS LQPSQWGMLC PSDTPEGEAC GLVKNLALMT
510 520 530 540 550
HITTDMEDGP IIKLAGNLGV EDVNLLCGEE LSYPNVFLVF LNGNILGVIR
560 570 580 590 600
DHKKLVSTFR LMRRAGYINE FVSISTNLTD RCVYISSDGG RLCRPYIIVK
610 620 630 640 650
KQKPAVTNKH MEELAQGYRN FEDFLHESLV EYLDVNEEND CNIALYEHTI
660 670 680 690 700
NKDTTHLEIE PFTLLGVCAG LIPYPHHNQS PRNTYQCAMG KQAMGTIGYN
710 720 730 740 750
QRNRIDTLMY LLAYPQKPMV KTKTIELIDF EKLPAGQNAT VAVMSYSGYD
760 770 780 790 800
IEDALVLNKA SLDRGFGRCL VYKNAKCTLK RYTNQTFDKV MGPMLDAATR
810 820 830 840 850
KPIWRHEILD ADGICSPGEK VENKQVLVNK SMPTVTQIPL EGSNVPQQPQ
860 870 880 890 900
YKDVPITYKG ATDSYIEKVM ISSNAEDAFL IKMLLRQTRR PEIGDKFSSR
910 920 930 940 950
HGQKGVCGLI VPQEDMPFCD SGICPDIIMN PHGFPSRMTV GKLIELLAGK
960 970 980 990 1000
AGVLDGRFHY GTAFGGSKVK DVCEDLVRHG YNYLGKDYVT SGITGEPLEA
1010 1020 1030 1040 1050
YIYFGPVYYQ KLKHMVLDKM HARARGPRAV LTRQPTEGRS RDGGLRLGEM
1060 1070 1080 1090 1100
ERDCLIGYGA SMLLLERLMI SSDAFEVDVC GQCGLLGYSG WCHYCKSSCH
1110 1120 1130
VSSLRIPYAC KLLFQELQSM NIIPRLKLAK YNE
Length:1,133
Mass (Da):127,715
Last modified:August 31, 2004 - v2
Checksum:i7B900B187F2753AF
GO

Sequence cautioni

The sequence AAH44796 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465V → E in BAC30327 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC044796 mRNA. Translation: AAH44796.1. Different initiation.
BC068143 mRNA. Translation: AAH68143.1.
AK039366 mRNA. Translation: BAC30327.1.
CCDSiCCDS24081.1.
RefSeqiNP_081699.1. NM_027423.1.
UniGeneiMm.335007.
Mm.472000.

Genome annotation databases

EnsembliENSMUST00000077175; ENSMUSP00000076418; ENSMUSG00000034453.
GeneIDi70428.
KEGGimmu:70428.
UCSCiuc007gku.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC044796 mRNA. Translation: AAH44796.1. Different initiation.
BC068143 mRNA. Translation: AAH68143.1.
AK039366 mRNA. Translation: BAC30327.1.
CCDSiCCDS24081.1.
RefSeqiNP_081699.1. NM_027423.1.
UniGeneiMm.335007.
Mm.472000.

3D structure databases

ProteinModelPortaliP59470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076418.

PTM databases

iPTMnetiP59470.
PhosphoSitePlusiP59470.

Proteomic databases

EPDiP59470.
MaxQBiP59470.
PaxDbiP59470.
PeptideAtlasiP59470.
PRIDEiP59470.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077175; ENSMUSP00000076418; ENSMUSG00000034453.
GeneIDi70428.
KEGGimmu:70428.
UCSCiuc007gku.1. mouse.

Organism-specific databases

CTDi55703.
MGIiMGI:1917678. Polr3b.

Phylogenomic databases

eggNOGiKOG0215. Eukaryota.
COG0085. LUCA.
GeneTreeiENSGT00860000133818.
HOGENOMiHOG000222962.
HOVERGENiHBG017744.
InParanoidiP59470.
KOiK03021.
OMAiPYIIVKN.
OrthoDBiEOG091G00PZ.
PhylomeDBiP59470.
TreeFamiTF103047.

Miscellaneous databases

PROiP59470.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034453.
CleanExiMM_POLR3B.
GenevisibleiP59470. MM.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.40.270.10. 2 hits.
2.40.50.150. 1 hit.
3.90.1110.10. 1 hit.
InterProiIPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007646. RNA_pol_Rpb2_4.
IPR007647. RNA_pol_Rpb2_5.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 1 hit.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF04566. RNA_pol_Rpb2_4. 1 hit.
PF04567. RNA_pol_Rpb2_5. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPC2_MOUSE
AccessioniPrimary (citable) accession number: P59470
Secondary accession number(s): Q6NVF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.