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Protein

DNA-directed RNA polymerase III subunit RPC2

Gene

Polr3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1080ZincBy similarity1
Metal bindingi1083ZincBy similarity1
Metal bindingi1092ZincBy similarity1
Metal bindingi1095ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processAntiviral defense, Immunity, Innate immunity, Transcription
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-MMU-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-MMU-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
C128
DNA-directed RNA polymerase III 127.6 kDa polypeptide
DNA-directed RNA polymerase III subunit B
Gene namesi
Name:Polr3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1917678 Polr3b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000480931 – 1133DNA-directed RNA polymerase III subunit RPC2Add BLAST1133

Proteomic databases

EPDiP59470
MaxQBiP59470
PaxDbiP59470
PeptideAtlasiP59470
PRIDEiP59470

PTM databases

iPTMnetiP59470
PhosphoSitePlusiP59470

Expressioni

Gene expression databases

BgeeiENSMUSG00000034453
CleanExiMM_POLR3B
ExpressionAtlasiP59470 baseline and differential
GenevisibleiP59470 MM

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000076418

Structurei

3D structure databases

ProteinModelPortaliP59470
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1080 – 1095C4-typeAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0215 Eukaryota
COG0085 LUCA
GeneTreeiENSGT00910000144264
HOGENOMiHOG000222962
HOVERGENiHBG017744
InParanoidiP59470
KOiK03021
OMAiMLCTSDT
OrthoDBiEOG091G00PZ
PhylomeDBiP59470
TreeFamiTF103047

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 3 hits
2.40.50.150, 2 hits
InterProiView protein in InterPro
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR007645 RNA_pol_Rpb2_3
IPR007646 RNA_pol_Rpb2_4
IPR007647 RNA_pol_Rpb2_5
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 1 hit
PF04565 RNA_pol_Rpb2_3, 1 hit
PF04566 RNA_pol_Rpb2_4, 1 hit
PF04567 RNA_pol_Rpb2_5, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
PROSITEiView protein in PROSITE
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

P59470-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVLAEEFGS LTPEQLTAPI PTVEEKWRLL PAFLKVKGLV KQHIDSFNYF
60 70 80 90 100
INVEIKKIMK ANEKVTSDAD PMWYLKYLNI YVGLPDVEES FNVTRPVSPH
110 120 130 140 150
ECRLRDMTYS APITVDIEYT RGSQRIIRNA LPIGRMPIML RSSNCVLTGK
160 170 180 190 200
TPAEFAKLNE CPLDPGGYFI VKGVEKVILI QEQLSKNRII VEADRKGAVG
210 220 230 240 250
ASVTSSTHEK KSRTNMAVKQ GRFYLRHNTL SEDIPIVIIF KAMGVESDQE
260 270 280 290 300
IVQMIGTEEH VMAAFGPSLE ECQKAQIFTQ MQALKYIGNK VRRQRMWGGG
310 320 330 340 350
PKKTKIEEAR ELLASTILTH VPVKEFNFRA KCIYTAVMVR RVILAQGDNK
360 370 380 390 400
VDDRDYYGNK RLELAGQLLS LLFEDLFKKF NSEMKKIADQ VIPKQRAAQF
410 420 430 440 450
DVVKHMRQDQ ITNGMVNAIS TGNWSLKRFK MDRQGVTQVL SRLSYISALG
460 470 480 490 500
MMTRISSQFE KTRKVSGPRS LQPSQWGMLC PSDTPEGEAC GLVKNLALMT
510 520 530 540 550
HITTDMEDGP IIKLAGNLGV EDVNLLCGEE LSYPNVFLVF LNGNILGVIR
560 570 580 590 600
DHKKLVSTFR LMRRAGYINE FVSISTNLTD RCVYISSDGG RLCRPYIIVK
610 620 630 640 650
KQKPAVTNKH MEELAQGYRN FEDFLHESLV EYLDVNEEND CNIALYEHTI
660 670 680 690 700
NKDTTHLEIE PFTLLGVCAG LIPYPHHNQS PRNTYQCAMG KQAMGTIGYN
710 720 730 740 750
QRNRIDTLMY LLAYPQKPMV KTKTIELIDF EKLPAGQNAT VAVMSYSGYD
760 770 780 790 800
IEDALVLNKA SLDRGFGRCL VYKNAKCTLK RYTNQTFDKV MGPMLDAATR
810 820 830 840 850
KPIWRHEILD ADGICSPGEK VENKQVLVNK SMPTVTQIPL EGSNVPQQPQ
860 870 880 890 900
YKDVPITYKG ATDSYIEKVM ISSNAEDAFL IKMLLRQTRR PEIGDKFSSR
910 920 930 940 950
HGQKGVCGLI VPQEDMPFCD SGICPDIIMN PHGFPSRMTV GKLIELLAGK
960 970 980 990 1000
AGVLDGRFHY GTAFGGSKVK DVCEDLVRHG YNYLGKDYVT SGITGEPLEA
1010 1020 1030 1040 1050
YIYFGPVYYQ KLKHMVLDKM HARARGPRAV LTRQPTEGRS RDGGLRLGEM
1060 1070 1080 1090 1100
ERDCLIGYGA SMLLLERLMI SSDAFEVDVC GQCGLLGYSG WCHYCKSSCH
1110 1120 1130
VSSLRIPYAC KLLFQELQSM NIIPRLKLAK YNE
Length:1,133
Mass (Da):127,715
Last modified:August 31, 2004 - v2
Checksum:i7B900B187F2753AF
GO

Sequence cautioni

The sequence AAH44796 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465V → E in BAC30327 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC044796 mRNA Translation: AAH44796.1 Different initiation.
BC068143 mRNA Translation: AAH68143.1
AK039366 mRNA Translation: BAC30327.1
CCDSiCCDS24081.1
RefSeqiNP_081699.1, NM_027423.1
UniGeneiMm.335007
Mm.472000

Genome annotation databases

EnsembliENSMUST00000077175; ENSMUSP00000076418; ENSMUSG00000034453
GeneIDi70428
KEGGimmu:70428
UCSCiuc007gku.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRPC2_MOUSE
AccessioniPrimary (citable) accession number: P59470
Secondary accession number(s): Q6NVF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: August 31, 2004
Last modified: March 28, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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