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P59466 (SYW_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:bbp_478
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136612

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140
Motif197 – 2015"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P59466 [UniParc].

Last modified March 28, 2003. Version 1.
Checksum: FA6A4B37CE94314D

FASTA33338,386
        10         20         30         40         50         60 
MEKLNKNVFS AVQPSGQLTI GNYIGVLREW ILMQHDFTCF FCIADLHSLT VRRSSAILKN 

        70         80         90        100        110        120 
SVLDVVATYL ACGLDPCKSV IFLQSCVHEH SELHWLLTCC TYYGELIRMT QFKDKARKNS 

       130        140        150        160        170        180 
KNINVGLLSY PILMASDVLL YDTDVVPIGL DQKQHMELIC NIARRFNLLY GNTFKIPKNK 

       190        200        210        220        230        240 
MSYYGSNILS LSDPTKKMSK SDVNENGVIF LLDNLSSVKK KIKLAVTDSD NPPRIRYDKD 

       250        260        270        280        290        300 
TKIGISNLIN ILSCFQNRDI SDIEKEFYYY SYQDFKSVVI KEIVNFMTNF QNLYFSFRKN 

       310        320        330 
EDYLEKILSE GSKRASWYAK KLLKKVKTSM GLF 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO27184.1.
RefSeqNP_778079.1. NC_004545.1.

3D structure databases

ProteinModelPortalP59466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO27184; AAO27184; bbp_478.
GeneID1058311.
KEGGbab:bbp478.
PATRIC21245773. VBIBucAph80364_0475.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAYFIPDMH.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-478-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_BUCBP
AccessionPrimary (citable) accession number: P59466
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries