P59450 (MURB_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	bbp_046

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179189
Regions
	Domain	16 – 186	171	FAD-binding PCMH-type
Sites
	Active site	162	1	By similarity
	Active site	232	1	Proton donor By similarity
	Active site	329	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59450 [UniParc]. Last modified March 28, 2003. Version 1. Checksum: C0703B4E5BA64B05	FASTA	334	38,428
	10 20 30 40 50 60 MKTYYTSLKK FHTFKINVFA KKIIIVKTIK TLIKTWKKCN QENLPFLLLG KGSNVLFTKN 70 80 90 100 110 120 YNGFVVVNRI SGITIHEQKD YWLLHVKGGT KWNNLVKYTI QKKIYGLENL ALIPGTVGAA 130 140 150 160 170 180 PIQNIGAYGV EFKDVCQYVD VLYLNNSKIV RINSNNCLFG YRDSIFKKKH NPNSIILSVG 190 200 210 220 230 240 IKLPKTWKPK ISHLELQKLS FKNITSHQIF HYICKLRKKK LPNPKKIGNA GSFFKNPIIK 250 260 270 280 290 300 KNKAHKIICE YKDLPFYPEP HGMIKLSAGW LIEKCKLKNF SVGNAKIYHK QALILINKNN 310 320 330 LATSKNIIKL AKIIISKVKK KFDITLELEV QIIN

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO26785.1.
RefSeq	NP_777680.1. NC_004545.1.
3D structure databases
ProteinModelPortal	P59450.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002560; EBBUCP00000002384; EBBUCG00000002560.
GeneID	1058361.
GenomeReviews	Gene locus bbp_046 in contig AE016826_GR.
KEGG	bab:bbp046.
PATRIC	21244865. VBIBucAph80364_0043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007998.
HOGENOM	HBG686573.
OMA	LAYEITS.
PhylomeDB	P59450.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_046-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BUCBP

Accession

Primary (citable) accession number: P59450

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program