Reviewed, UniProtKB/Swiss-Prot P59443 (GUAC_BUCBP)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	bbp_188

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	GMP reductase		PRO_0000093734
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59443-1 [UniParc]. Last modified March 28, 2003. Version 1. Checksum: 67FEEFB53391DD81	FASTA	351	38,564
	10 20 30 40 50 60 MRIEEDIKLG FKDVLIRPKR SILKSRSQVD LTRLFIFKHA QNKWSGIPLI AANMDTVGTF 70 80 90 100 110 120 KMAIALSSFG ILTAIHKYYS YLDWKKFIHT IPGTVVKHIM VSTGMLDEDF VKLKQILSLS 130 140 150 160 170 180 SKLKYICIDV ANGYSEKFVT FLKKVRECYC DKIICAGNVV TGEMVEELIL SGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRA KTAIGYPQLS AVIECSDAAH GLGGQIISDG GCVVSGDIAK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHKE CEGLIFKENK KRYMIFYGMS SKFAMDRHIG GVARYKTPEG KTVKVLFRGA 310 320 330 340 350 VSETINNILG GLRSTCTYVG AFTLKELTKR TTFIKVSEQE NIIFNNQEVS N

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26920.1.
RefSeq	NP_777815.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
ModBase	Search...
Genome annotation databases
GeneID	1058572.
GenomeReviews	Gene locus bbp_188 in contig AE016826_GR.
KEGG	bab:bbp188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59443.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_188-MON.
Family and domain databases
HAMAP	MF_00596. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BUCBP

Accession

Primary (citable) accession number: P59443

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	November 4, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)