Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P59435 (DDL_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlB
Ordered Locus Names:bbp_196
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177799

Regions

Domain104 – 306203ATP-grasp
Nucleotide binding137 – 19256ATP By similarity

Sites

Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2731Magnesium or manganese 1 By similarity
Metal binding2731Magnesium or manganese 2 By similarity
Metal binding2751Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P59435 [UniParc].

Last modified March 28, 2003. Version 1.
Checksum: 54992D5CE0CBF323

FASTA30934,986
        10         20         30         40         50         60 
MTIITEKIAV LLGGTSQERN ISLISGYNIL NSLLKSGIHA VAIDTKDFPI TQLPHQKFTK 

        70         80         90        100        110        120 
AFIALHGRDG EDGTIQSVLK YLNIPFTGSK TLPSAISINK FKTKLLWQSF NLPVVPYLHI 

       130        140        150        160        170        180 
NKHEFNKKFL QKFKKNISLL GLPIIVKPNQ EGSSIGITIV YSYETLYKAC KTAFIFDNSI 

       190        200        210        220        230        240 
LIEKFIYGEE YTISILGKKI LPIIRICPEN TFYNYNSKYL SNRTTYFCPS GLNKLKELEL 

       250        260        270        280        290        300 
KKITLTAWNI IDGTGWGRVD VIMDYKNKFW LLEANTCPGM TDHSLFPMSA KKAGISYQIL 


VQKILELAN 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO26928.1.
RefSeqNP_777823.1. NC_004545.1.

3D structure databases

ProteinModelPortalP59435.
SMRP59435. Positions 5-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO26928; AAO26928; bbp_196.
GeneID1058244.
KEGGbab:bbp196.
PATRIC21245167. VBIBucAph80364_0190.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-196-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BUCBP
AccessionPrimary (citable) accession number: P59435
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways