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P59433 (SYL_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:bbp_395
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151989

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P59433 [UniParc].

Last modified March 28, 2003. Version 1.
Checksum: 35F7B75BE5A45012

FASTA861101,600
        10         20         30         40         50         60 
MKQNYCPKTI EPYVQSIWKK KNTFKVTENS NKEKFYCLAM IPYPSGKLHM GHVRNYTISD 

        70         80         90        100        110        120 
VIARYQRMLG KNVLHPMGWD AFGLPAENAA IKNNTHPAQW TYENIKYMKQ QLISLGLSYD 

       130        140        150        160        170        180 
WDREITTCKP EYYQWEQWFF IELYKKNLVY KKKSWVNWCE YDKTVLANEQ VINELCWRCN 

       190        200        210        220        230        240 
NKVIKKKIFQ WFIKITKYAE ELLNDLDNLP EWPEKVKTMQ HNWIGRNHGI KIKLKLANQH 

       250        260        270        280        290        300 
TILNDVFISK PSTLMGATFI TLSPSHELSF KIARKKHKIQ EFIENCSSNT NTYNDINNTN 

       310        320        330        340        350        360 
IGINTNEFAL HPITKKKLPI WITNYVLSDY DTNSILCVPA HNQHDLNFAI KYNLKIKAVI 

       370        380        390        400        410        420 
LNLDGTEPKI KNTAMTSMGK LFNSNQYNNL NYQEGSYRII QDLENNHIGK KITYYRLRDW 

       430        440        450        460        470        480 
SISRQRYWGA PIPMAVLENK KNVPIPKQYL PIILPETIPF KNIKPLSNNI LLKKIYIDEK 

       490        500        510        520        530        540 
IAICESDTFD TFLESSWYYA RFTCNNFHKG MISQKLANYW LPVDQYIGGI EHAVMHLIYF 

       550        560        570        580        590        600 
RFVHKLLRDL GLVYSNEPVK KLLCQGMVLS DAFYYFDHNK QKQWISAKSI TIKYDSNHKI 

       610        620        630        640        650        660 
QSHFYSNNKK IFHAGMIKMS KSKFNGIEPE DIIKKYGTDT IRLFIMFAAP VESALEWKES 

       670        680        690        700        710        720 
GVKGIHKFLK KLWVLSYNHI KLYNHKIKLR INLFTEQQQH IYSELHKTIK IVSQYITDTQ 

       730        740        750        760        770        780 
SFNVAISKIM KFSNTLMSIS LKNEQNQALM QESLLAVIQM LYPFIPHFSF AIWEFLSPKK 

       790        800        810        820        830        840 
ENIDFISWPK YNFKAILSKL KYTIIIQING KKRHKILALK NSSQEKILEI ILNENKIKKY 

       850        860 
LNNKPIQKII YIPNKILNLV I 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO27107.1.
RefSeqNP_778002.1. NC_004545.1.

3D structure databases

ProteinModelPortalP59433.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO27107; AAO27107; bbp_395.
GeneID1058295.
KEGGbab:bbp395.
PATRIC21245593. VBIBucAph80364_0390.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-395-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BUCBP
AccessionPrimary (citable) accession number: P59433
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries