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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-395-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:bbp_395
OrganismiBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
Taxonomic identifieri224915 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000601 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Leucine--tRNA ligasePRO_0000151989Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224915.bbp395.

Structurei

3D structure databases

ProteinModelPortaliP59433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQNYCPKTI EPYVQSIWKK KNTFKVTENS NKEKFYCLAM IPYPSGKLHM
60 70 80 90 100
GHVRNYTISD VIARYQRMLG KNVLHPMGWD AFGLPAENAA IKNNTHPAQW
110 120 130 140 150
TYENIKYMKQ QLISLGLSYD WDREITTCKP EYYQWEQWFF IELYKKNLVY
160 170 180 190 200
KKKSWVNWCE YDKTVLANEQ VINELCWRCN NKVIKKKIFQ WFIKITKYAE
210 220 230 240 250
ELLNDLDNLP EWPEKVKTMQ HNWIGRNHGI KIKLKLANQH TILNDVFISK
260 270 280 290 300
PSTLMGATFI TLSPSHELSF KIARKKHKIQ EFIENCSSNT NTYNDINNTN
310 320 330 340 350
IGINTNEFAL HPITKKKLPI WITNYVLSDY DTNSILCVPA HNQHDLNFAI
360 370 380 390 400
KYNLKIKAVI LNLDGTEPKI KNTAMTSMGK LFNSNQYNNL NYQEGSYRII
410 420 430 440 450
QDLENNHIGK KITYYRLRDW SISRQRYWGA PIPMAVLENK KNVPIPKQYL
460 470 480 490 500
PIILPETIPF KNIKPLSNNI LLKKIYIDEK IAICESDTFD TFLESSWYYA
510 520 530 540 550
RFTCNNFHKG MISQKLANYW LPVDQYIGGI EHAVMHLIYF RFVHKLLRDL
560 570 580 590 600
GLVYSNEPVK KLLCQGMVLS DAFYYFDHNK QKQWISAKSI TIKYDSNHKI
610 620 630 640 650
QSHFYSNNKK IFHAGMIKMS KSKFNGIEPE DIIKKYGTDT IRLFIMFAAP
660 670 680 690 700
VESALEWKES GVKGIHKFLK KLWVLSYNHI KLYNHKIKLR INLFTEQQQH
710 720 730 740 750
IYSELHKTIK IVSQYITDTQ SFNVAISKIM KFSNTLMSIS LKNEQNQALM
760 770 780 790 800
QESLLAVIQM LYPFIPHFSF AIWEFLSPKK ENIDFISWPK YNFKAILSKL
810 820 830 840 850
KYTIIIQING KKRHKILALK NSSQEKILEI ILNENKIKKY LNNKPIQKII
860
YIPNKILNLV I
Length:861
Mass (Da):101,600
Last modified:March 28, 2003 - v1
Checksum:i35F7B75BE5A45012
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27107.1.
RefSeqiNP_778002.1. NC_004545.1.

Genome annotation databases

EnsemblBacteriaiAAO27107; AAO27107; bbp_395.
KEGGibab:bbp395.
PATRICi21245593. VBIBucAph80364_0390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016826 Genomic DNA. Translation: AAO27107.1.
RefSeqiNP_778002.1. NC_004545.1.

3D structure databases

ProteinModelPortaliP59433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224915.bbp395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO27107; AAO27107; bbp_395.
KEGGibab:bbp395.
PATRICi21245593. VBIBucAph80364_0390.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBAPH224915:GJ9D-395-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bp.

Entry informationi

Entry nameiSYL_BUCBP
AccessioniPrimary (citable) accession number: P59433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: April 29, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.