P59426 (ILVD_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	bbp_542

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103448
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P59426 [UniParc]. Last modified March 28, 2003. Version 1. Checksum: B68569C40606E5FD	FASTA	612	66,843
	10 20 30 40 50 60 MPKYRSSTTT QGPNMSGARA LWRATGMSSD DFNKPIIAVV NSFTEFVPGH IHLRELGQLV 70 80 90 100 110 120 SQYIISSGGV SKEFNTIAID DGIAMGHSGM LYSLPSRDLI ADSVEFMINA HCVDAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPC VFVSGGPMES GKIVISDKEI KLNLVDAITC GVNPDSSDDM 190 200 210 220 230 240 INEIEKSACP TCGSCSGMFT ANSMNCLMEA IGLSLPGNGT ILATHIDRKK LFVQAGKLIV 250 260 270 280 290 300 KITQEYYKNN NEKFLPRNVA TKEAFCNAMA LDIAMGGSTN TVLHLLAAAR EGCVDFTMLD 310 320 330 340 350 360 VDLLSRKIPN LCKLSPNTTF YHIEDFHRAG GVFGVLDELN KVNLLYKQTF NILGLNLEEM 370 380 390 400 410 420 LSKYSILTGN DKNIIKMFYA APSGQKTIKP FSQSCRWNSL DIDRKLGCIR DYNSAFSLDG 430 440 450 460 470 480 GIAVLYGNLA KDGCIVKTAG VHEKNLIFRG KAVVYESQDE AVHAILNNKI VKGNVIIIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSYLK SMKLDQDCAL ITDGRFSGGT SGLSIGHISP EAANQGIIAL 550 560 570 580 590 600 VRTGDFIDIN IPNRKIHLDI TDNELLLRIK NEKDRGIDAY TPSHRKRIVT SALKLYSKFV 610 TSADKGAVRN IR

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27241.1.
RefSeq	NP_778136.1. NC_004545.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002508; EBBUCP00000002332; EBBUCG00000002508.
GeneID	1058331.
GenomeReviews	Gene locus bbp_542 in contig AE016826_GR.
KEGG	bab:bbp542.
PATRIC	21245909. VBIBucAph80364_0536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008100.
HOGENOM	HBG671001.
OMA	MALVIRY.
PhylomeDB	P59426.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_542-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BUCBP

Accession

Primary (citable) accession number: P59426

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program