Reviewed, UniProtKB/Swiss-Prot P59417 (IPYR_BUCBP)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	bbp_082

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137486
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59417-1 [UniParc]. Last modified March 28, 2003. Version 1. Checksum: 484EC9C75DF808EF	FASTA	175	20,100
	10 20 30 40 50 60 MTLNTVLSGK NIPEDIYVII EISAHSNPIK YEVNKDLGLL FVDRFIPVPM FYPCNYGFIN 70 80 90 100 110 120 KTKSNDGDPL DVLVHTPYPI LPGSIIRCKP IGVLNMFDES GEDYKIIAIP HPKVCLEYSS 130 140 150 160 170 INDICHLSPT LKQQIIHFFK HYKNLENNKW VKIIGWENKK NAEKIILSAC EKFHK

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26818.1.
RefSeq	NP_777713.1.
3D structure databases
HSSP	HSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBase	Search...
Genome annotation databases
GeneID	1058391.
GenomeReviews	Gene locus bbp_082 in contig AE016826_GR.
KEGG	bab:bbp082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59417.
OMA	EIDKDTG.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_082-MON.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BUCBP

Accession

Primary (citable) accession number: P59417

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)