Reviewed, UniProtKB/Swiss-Prot P59412 (ASSY_BUCBP)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	bbp_048

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148578
Regions
	Nucleotide binding	12 – 20	9	ATP By similarity
Sites
	Binding site	39	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	91	1	Citrulline By similarity
	Binding site	121	1	ATP; via amide nitrogen By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	128	1	Aspartate By similarity
	Binding site	131	1	Citrulline By similarity
	Binding site	180	1	Citrulline By similarity
	Binding site	189	1	Citrulline By similarity
	Binding site	265	1	Citrulline By similarity
	Binding site	277	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59412-1 [UniParc]. Last modified March 28, 2003. Version 1. Checksum: 1257088969D05697	FASTA	409	46,032
	10 20 30 40 50 60 MRNKNIKKVV LAYSGGLDTS AIIPWIKENY SSEVIAFVAN VGQNQADLKD IEYKALKSGA 70 80 90 100 110 120 SQCIIKDLRE EFIRDYVYPV LKSGALYEEN YLLGTALARP IIAKSQVDLA LKLKADGVCH 130 140 150 160 170 180 GATGKGNDQV RFETAYVGLA PELKIIAPWR EWSFKSRGEL LSYLKEKNVK TTASIEKIYS 190 200 210 220 230 240 KDENAWHVST EGGVLEDLWN KPNQDCWTWT NDPKDAPNVP EIISIKIKNG SVIAVNNEFL 250 260 270 280 290 300 SPFKCLEKLN LVGIKHGIGR IDVVENRLVG MKSRACYETP GGTILVNALR SLEQLVLDRD 310 320 330 340 350 360 SYKWRQQIAL EMSYVIYNGN WFSPFRKSLQ AAATELATEI DGEVILELYK GTVTAIRKFS 370 380 390 400 PNSLYSKEFA TFDQDEVYRQ SDADGFIKLY SLPSKIRAIN KCMNKALMK

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO26787.1.
RefSeq	NP_777682.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1058536.
GenomeReviews	Gene locus bbp_048 in contig AE016826_GR.
KEGG	bab:bbp048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59412.
OMA	IAPVREW.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_048-MON.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BUCBP

Accession

Primary (citable) accession number: P59412

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)