P59390 (LDH2_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 2
Short name=L-LDH 2
EC=1.1.1.27

Gene names

Name:	ldh2
Synonyms:	ldhL2
Ordered Locus Names:	lp_1101

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	L-lactate dehydrogenase 2 HAMAP MF_00488		PRO_0000168358
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	122	1	NAD or substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59390 [UniParc]. Last modified March 25, 2003. Version 1. Checksum: A0DF444AC833060D	FASTA	309	33,116
	10 20 30 40 50 60 MDKKQRKVVI VGDGSVGSSF AFSLVQNCAL DELVIVDLVK THAEGDVKDL EDVAAFTNAT 70 80 90 100 110 120 NIHTGEYADA RDADIVVITA GVPRKPGESR LDLINRNTKI LESIVKPVVA SGFNGCFVIS 130 140 150 160 170 180 SNPVDILTSM TQRLSGFPRH RVIGTGTSLD TARLRVALAQ KLNVATTAVD AAVLGEHGDS 190 200 210 220 230 240 SIVNFDEIMI NAQPLKTVTT VDDQFKAEIE QAVRGKGGQI ISQKGATFYG VAVSLMQICR 250 260 270 280 290 300 AILNDENAEL IVSAALSGQY GINDLYLGSP AIINRNGLQK VIEAELSDDE RARMQHFAAK MLTMMNVAS

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC78502.1.
RefSeq	NP_784782.1. NC_004567.1.
3D structure databases
ProteinModelPortal	P59390.
SMR	P59390. Positions 4-304.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1063343.
GenomeReviews	Gene locus lp_1101 in contig AL935263_GR.
KEGG	lpl:lp_1101.
NMPDR	fig|220668.1.peg.916.
PATRIC	22248535. VBILacPla27411_0931.
Phylogenomic databases
HOGENOM	HBG566126.
OMA	NCALDEL.
ProtClustDB	PRK00066.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_1101-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LACPL

Accession

Primary (citable) accession number: P59390
Secondary accession number(s): F9UMR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 25, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program