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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityBy similarity
Active sitei603 – 6031For Fru-6P isomerization activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4639-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:bll4607
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 608607Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135307Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224911.bll4607.

Structurei

3D structure databases

ProteinModelPortaliP59362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 217216Glutamine amidotransferase type-2Add
BLAST
Domaini284 – 423140SIS 1Add
BLAST
Domaini456 – 598143SIS 2Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.Curated

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
InParanoidiP59362.
KOiK00820.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP59362.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGILGR EPVAEQLVDS LKRLEYRGYD SAGVATLEGR HLERRRAEGK
60 70 80 90 100
LKNLEKRLEA EPLKGTTGIG HTRWATHGKP TVNNAHPHAT ERVAVVHNGI
110 120 130 140 150
IENFRELREE LEKNGTVFHT ETDTEIVLHL VDDLLTRGNK PVEAVKLALA
160 170 180 190 200
RLRGAFALGF IFAGDNDLMI GARNGPPLAI GYGDGEMYLG SDAIALGPFT
210 220 230 240 250
DTISYLEDGD WVVLTRNSAT IFDKDGHAVQ REKIKHAAST SLVDKANYRH
260 270 280 290 300
FMAKEIHEQP EVVGHTLARY VDMATERVSL PVKLPFDFKN IQRINITACG
310 320 330 340 350
TASYAGFVAK YWFERFARLP VEVDVASEFR YREAPLRKGD LAIFISQSGE
360 370 380 390 400
TADTLAALRY AKAEGVHTVA VVNVPTSTIA RESETVLPTL AGPEIGVAST
410 420 430 440 450
KAFTCQLMVL ANLAIAAGKA RGELSDEDET KLVHGLVEIP RLMSDALTTE
460 470 480 490 500
LQIEKLAREI AKSRDVLYLG RGTSFPLALE GALKLKEISY IHAEGYAAGE
510 520 530 540 550
LKHGPIALID ETMPVVVIAP YDRVFEKTVS NMQEVAARGG KIILMTDAKG
560 570 580 590 600
AEEATVESLV TIVMPDMAAA FTPMVYAVPV QLLAYHTAVI MGTDVDQPRN

LAKSVTVE
Length:608
Mass (Da):66,370
Last modified:January 22, 2007 - v2
Checksum:iA2627F79781AF783
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC49872.1.
RefSeqiNP_771247.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC49872; BAC49872; BAC49872.
GeneIDi1052463.
KEGGibja:bll4607.
PATRICi21192848. VBIBraJap65052_4674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC49872.1.
RefSeqiNP_771247.1. NC_004463.1.

3D structure databases

ProteinModelPortaliP59362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll4607.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC49872; BAC49872; BAC49872.
GeneIDi1052463.
KEGGibja:bll4607.
PATRICi21192848. VBIBraJap65052_4674.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
InParanoidiP59362.
KOiK00820.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP59362.

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4639-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLMS_BRADU
AccessioniPrimary (citable) accession number: P59362
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 27, 2003
Last sequence update: January 22, 2007
Last modified: January 6, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.