Reviewed, UniProtKB/Swiss-Prot P59342 (BARA_SHIFL)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase barA
    EC=2.7.13.3

Gene names

Name:	barA
Ordered Locus Names:	SF2799, S2993

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system uvrY/barA involved in the regulation of carbon metabolism via the csrA/csrB regulatory system. Phosphorylates uvrY, probably via a four-step phosphorelay By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 HPt domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Signal transduction histidine-protein kinase barA		PRO_0000074700
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Potential
	Topological domain	32 – 175	144	Periplasmic Potential
	Transmembrane	176 – 196	21	Potential
	Topological domain	197 – 918	722	Cytoplasmic Potential
	Domain	200 – 252	53	HAMP
	Domain	299 – 520	222	Histidine kinase
	Domain	669 – 785	117	Response regulatory
	Domain	822 – 918	97	HPt
Amino acid modifications
	Modified residue	302	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	718	1	4-aspartylphosphate By similarity
	Modified residue	861	1	Phosphohistidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59342-1 [UniParc]. Last modified February 22, 2003. Version 1. Checksum: 2E95844A64D3A842	FASTA	918	102,481
	10 20 30 40 50 60 MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE PLAVSTEYGM 70 80 90 100 110 120 SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN FHLDPSSMQL GSNVPFPRQL 130 140 150 160 170 180 TVTRDGDIMI LRTPIISESY SPDESPSSDA KNSQNMLGYI ALELDLKSVR LQQYKEIFIS 190 200 210 220 230 240 CVMMLFCIGI ALIFGWRLMR DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI 250 260 270 280 290 300 NSMAMSLAAY HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM 310 320 330 340 350 360 SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD FSKLEAGKLI 370 380 390 400 410 420 LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD NVIGDPLRLQ QIITNLVGNA 430 440 450 460 470 480 IKFTENGNID ILVEKRALSN TKVQIEVQIR DTGIGIPERD QSRLFQAFRQ ADASISRRHG 490 500 510 520 530 540 GTGLGLVITQ KLVNEMGGDI SFHSQPNRGS TFWFHINLDL NPNIIIEGPS IQCLAGKRLA 550 560 570 580 590 600 YVEPNSAAAQ CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA 610 620 630 640 650 660 KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC HHKQNTLLPV 670 680 690 700 710 720 TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG HQAVERAKQM PFDLILMDIQ 730 740 750 760 770 780 MPDMDGIRAC ELIHQLPHQR QTPVIAVTAH AMAGQKEKLL GAGMSDYLAK PIEEERLHNL 790 800 810 820 830 840 LLRYKPGSGI SSRVVTPEVN EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV 850 860 870 880 890 900 RNKVEEQLAG ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL 910 ELLDEMDNVA REASKILG

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44287.1. AE014073 Genomic DNA. Translation: AAP18112.1.
RefSeq	NP_708580.1. NP_838302.1.
3D structure databases
HSSP	HSSP built from PDB template 1MAV based on UniProtKB Q9A5I4.
ModBase	Search...
Genome annotation databases
GeneID	1026816. 1079258.
GenomeReviews	Gene locus SF2799 in contig AE005674_GR. Gene locus S2993 in contig AE014073_GR.
KEGG	sfl:SF2799. sfx:S2993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59342.
OMA	VESAANQ.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44287.1-MON.
BRENDA	2.7.13.3. 189495.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR019247. DUF2222. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_P-trf. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF09984. DUF2222. 1 hit. PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF01627. Hpt. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00073. HPT. 1 hit. SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BARA_SHIFL

Accession

Primary (citable) accession number: P59342

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	February 22, 2003
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)