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Protein

Sensor histidine kinase DcuS

Gene

dcuS

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107C4-dicarboxylateBy similarity1
Binding sitei110C4-dicarboxylateBy similarity1
Binding sitei121C4-dicarboxylate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei147C4-dicarboxylateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL199310:C5131-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DcuS (EC:2.7.13.3)
Gene namesi
Name:dcuS
Ordered Locus Names:c5131
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 181PeriplasmicSequence analysisAdd BLAST140
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 543CytoplasmicSequence analysisAdd BLAST341

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747521 – 543Sensor histidine kinase DcuSAdd BLAST543

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei349Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated. The phosphoryl group is rapidly transferred to DcuR (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi199310.c5131.

Structurei

3D structure databases

ProteinModelPortaliP59340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 323PASPROSITE-ProRule annotationAdd BLAST112
Domaini346 – 538Histidine kinasePROSITE-ProRule annotationAdd BLAST193

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142C4-dicarboxylate bindingBy similarity3

Domaini

The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase (By similarity).By similarity

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
KOiK07701.
OMAiHYQNGWL.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD
60 70 80 90 100
GLANKALAVA RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV
110 120 130 140 150
TDMHSLRYSH PEAQRIGQPF KGDDILKALN GEENVAINRG FLAQALRVFT
160 170 180 190 200
PIYDENHKQI GVVAIGLELS RVTQQINDSR WSIIWSVLFG MLVGLIGTCI
210 220 230 240 250
LVNVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD RGEVTLINDA
260 270 280 290 300
AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL
310 320 330 340 350
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE
360 370 380 390 400
FMNKLHVILG LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF
410 420 430 440 450
LISKINRATD LGHTLILNSE SQLPDSGSED QVATLITTLG NLIENALEAL
460 470 480 490 500
GPEPGGEISV TLHYRHGWLH CEVNDDGPGI APDKIDHIFD KGVSTKGSER
510 520 530 540
GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER SNR
Length:543
Mass (Da):60,546
Last modified:February 22, 2003 - v1
Checksum:i201AB6FA1FF2B5CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN83553.1.
RefSeqiWP_001216466.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN83553; AAN83553; c5131.
KEGGiecc:c5131.
PATRICi18287942. VBIEscCol75197_4817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN83553.1.
RefSeqiWP_001216466.1. NC_004431.1.

3D structure databases

ProteinModelPortaliP59340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c5131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN83553; AAN83553; c5131.
KEGGiecc:c5131.
PATRICi18287942. VBIEscCol75197_4817.

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
KOiK07701.
OMAiHYQNGWL.

Enzyme and pathway databases

BioCyciECOL199310:C5131-MONOMER.
BRENDAi2.7.13.3. 2026.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCUS_ECOL6
AccessioniPrimary (citable) accession number: P59340
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: February 22, 2003
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.