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Protein

Haloalkane dehalogenase

Gene

dhaA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons.UniRule annotation

Catalytic activityi

1-haloalkane + H2O = a primary alcohol + halide.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103NucleophileUniRule annotation1
Active sitei127Proton donorUniRule annotation1
Active sitei280Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.8.1.5. 929.

Protein family/group databases

ESTHERibraja-dhaa. Haloalkane_dehalogenase-HLD2.

Names & Taxonomyi

Protein namesi
Recommended name:
Haloalkane dehalogenaseUniRule annotation (EC:3.8.1.5UniRule annotation)
Gene namesi
Name:dhaAUniRule annotation
Ordered Locus Names:blr1087
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002167801 – 310Haloalkane dehalogenaseAdd BLAST310

Interactioni

Subunit structurei

Monomer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6967845,EBI-6967845

Protein-protein interaction databases

MINTiMINT-8183161.
STRINGi224911.blr1087.

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 14Combined sources4
Beta strandi17 – 25Combined sources9
Beta strandi27 – 29Combined sources3
Beta strandi31 – 35Combined sources5
Helixi42 – 45Combined sources4
Turni46 – 48Combined sources3
Helixi49 – 52Combined sources4
Turni53 – 55Combined sources3
Beta strandi56 – 61Combined sources6
Helixi78 – 91Combined sources14
Beta strandi96 – 102Combined sources7
Helixi104 – 115Combined sources12
Turni117 – 119Combined sources3
Beta strandi120 – 127Combined sources8
Beta strandi132 – 134Combined sources3
Helixi135 – 137Combined sources3
Helixi144 – 146Combined sources3
Helixi147 – 160Combined sources14
Helixi165 – 170Combined sources6
Helixi175 – 178Combined sources4
Turni179 – 181Combined sources3
Helixi182 – 184Combined sources3
Helixi191 – 198Combined sources8
Helixi205 – 207Combined sources3
Helixi208 – 212Combined sources5
Helixi214 – 216Combined sources3
Helixi224 – 239Combined sources16
Beta strandi244 – 251Combined sources8
Beta strandi253 – 255Combined sources3
Helixi257 – 266Combined sources10
Beta strandi267 – 278Combined sources12
Helixi282 – 303Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A2LX-ray1.78A/B1-310[»]
3A2MX-ray1.84A/B1-310[»]
3A2NX-ray1.89A/B/E/F1-310[»]
3AFIX-ray1.75A/B/E/F1-310[»]
ProteinModelPortaliP59337.
SMRiP59337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59337.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 140AB hydrolase-1Sequence analysisAdd BLAST111

Sequence similaritiesi

Belongs to the haloalkane dehalogenase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JSS. Bacteria.
ENOG410ZVH8. LUCA.
HOGENOMiHOG000051838.
InParanoidiP59337.
KOiK01563.
OMAiAFFESHI.
OrthoDBiPOG091H0E7A.
PhylomeDBiP59337.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P59337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPIEIEIR RAPVLGSSMA YRETGAQDAP VVLFLHGNPT SSHIWRNILP
60 70 80 90 100
LVSPVAHCIA PDLIGFGQSG KPDIAYRFFD HVRYLDAFIE QRGVTSAYLV
110 120 130 140 150
AQDWGTALAF HLAARRPDFV RGLAFMEFIR PMPTWQDFHH TEVAEEQDHA
160 170 180 190 200
EAARAVFRKF RTPGEGEAMI LEANAFVERV LPGGIVRKLG DEEMAPYRTP
210 220 230 240 250
FPTPESRRPV LAFPRELPIA GEPADVYEAL QSAHAALAAS SYPKLLFTGE
260 270 280 290 300
PGALVSPEFA ERFAASLTRC ALIRLGAGLH YLQEDHADAI GRSVAGWIAG
310
IEAVRPQLAA
Length:310
Mass (Da):34,089
Last modified:February 22, 2003 - v1
Checksum:i7661684F109CF0FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46352.1.
RefSeqiNP_767727.1. NC_004463.1.
WP_011083907.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC46352; BAC46352; BAC46352.
GeneIDi1051963.
KEGGibja:blr1087.
PATRICi21185702. VBIBraJap65052_1122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46352.1.
RefSeqiNP_767727.1. NC_004463.1.
WP_011083907.1. NZ_CP011360.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A2LX-ray1.78A/B1-310[»]
3A2MX-ray1.84A/B1-310[»]
3A2NX-ray1.89A/B/E/F1-310[»]
3AFIX-ray1.75A/B/E/F1-310[»]
ProteinModelPortaliP59337.
SMRiP59337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8183161.
STRINGi224911.blr1087.

Protein family/group databases

ESTHERibraja-dhaa. Haloalkane_dehalogenase-HLD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46352; BAC46352; BAC46352.
GeneIDi1051963.
KEGGibja:blr1087.
PATRICi21185702. VBIBraJap65052_1122.

Phylogenomic databases

eggNOGiENOG4108JSS. Bacteria.
ENOG410ZVH8. LUCA.
HOGENOMiHOG000051838.
InParanoidiP59337.
KOiK01563.
OMAiAFFESHI.
OrthoDBiPOG091H0E7A.
PhylomeDBiP59337.

Enzyme and pathway databases

BRENDAi3.8.1.5. 929.

Miscellaneous databases

EvolutionaryTraceiP59337.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHAA_BRADU
AccessioniPrimary (citable) accession number: P59337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: February 22, 2003
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.