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Protein

Haloalkane dehalogenase

Gene

dhaA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons.UniRule annotation

Catalytic activityi

1-haloalkane + H2O = a primary alcohol + halide.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031NucleophileUniRule annotation
Active sitei127 – 1271Proton donorUniRule annotation
Active sitei280 – 2801Proton acceptorUniRule annotation

GO - Molecular functioni

  • haloalkane dehalogenase activity Source: GO_Central
  • identical protein binding Source: IntAct

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1096-MONOMER.
BRENDAi3.8.1.5. 929.

Protein family/group databases

ESTHERibraja-dhaa. Haloalkane_dehalogenase-HLD2.

Names & Taxonomyi

Protein namesi
Recommended name:
Haloalkane dehalogenaseUniRule annotation (EC:3.8.1.5UniRule annotation)
Gene namesi
Name:dhaAUniRule annotation
Ordered Locus Names:blr1087
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Haloalkane dehalogenasePRO_0000216780Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6967845,EBI-6967845

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

MINTiMINT-8183161.
STRINGi224911.blr1087.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 144Combined sources
Beta strandi17 – 259Combined sources
Beta strandi27 – 293Combined sources
Beta strandi31 – 355Combined sources
Helixi42 – 454Combined sources
Turni46 – 483Combined sources
Helixi49 – 524Combined sources
Turni53 – 553Combined sources
Beta strandi56 – 616Combined sources
Helixi78 – 9114Combined sources
Beta strandi96 – 1027Combined sources
Helixi104 – 11512Combined sources
Turni117 – 1193Combined sources
Beta strandi120 – 1278Combined sources
Beta strandi132 – 1343Combined sources
Helixi135 – 1373Combined sources
Helixi144 – 1463Combined sources
Helixi147 – 16014Combined sources
Helixi165 – 1706Combined sources
Helixi175 – 1784Combined sources
Turni179 – 1813Combined sources
Helixi182 – 1843Combined sources
Helixi191 – 1988Combined sources
Helixi205 – 2073Combined sources
Helixi208 – 2125Combined sources
Helixi214 – 2163Combined sources
Helixi224 – 23916Combined sources
Beta strandi244 – 2518Combined sources
Beta strandi253 – 2553Combined sources
Helixi257 – 26610Combined sources
Beta strandi267 – 27812Combined sources
Helixi282 – 30322Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A2LX-ray1.78A/B1-310[»]
3A2MX-ray1.84A/B1-310[»]
3A2NX-ray1.89A/B/E/F1-310[»]
3AFIX-ray1.75A/B/E/F1-310[»]
ProteinModelPortaliP59337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59337.

Family & Domainsi

Sequence similaritiesi

Belongs to the haloalkane dehalogenase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JSS. Bacteria.
ENOG410ZVH8. LUCA.
HOGENOMiHOG000051838.
InParanoidiP59337.
KOiK01563.
OrthoDBiEOG64FKGZ.
PhylomeDBiP59337.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P59337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPIEIEIR RAPVLGSSMA YRETGAQDAP VVLFLHGNPT SSHIWRNILP
60 70 80 90 100
LVSPVAHCIA PDLIGFGQSG KPDIAYRFFD HVRYLDAFIE QRGVTSAYLV
110 120 130 140 150
AQDWGTALAF HLAARRPDFV RGLAFMEFIR PMPTWQDFHH TEVAEEQDHA
160 170 180 190 200
EAARAVFRKF RTPGEGEAMI LEANAFVERV LPGGIVRKLG DEEMAPYRTP
210 220 230 240 250
FPTPESRRPV LAFPRELPIA GEPADVYEAL QSAHAALAAS SYPKLLFTGE
260 270 280 290 300
PGALVSPEFA ERFAASLTRC ALIRLGAGLH YLQEDHADAI GRSVAGWIAG
310
IEAVRPQLAA
Length:310
Mass (Da):34,089
Last modified:February 22, 2003 - v1
Checksum:i7661684F109CF0FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46352.1.
RefSeqiNP_767727.1. NC_004463.1.
WP_011083907.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46352; BAC46352; BAC46352.
GeneIDi1051963.
KEGGibja:blr1087.
PATRICi21185702. VBIBraJap65052_1122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46352.1.
RefSeqiNP_767727.1. NC_004463.1.
WP_011083907.1. NC_004463.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A2LX-ray1.78A/B1-310[»]
3A2MX-ray1.84A/B1-310[»]
3A2NX-ray1.89A/B/E/F1-310[»]
3AFIX-ray1.75A/B/E/F1-310[»]
ProteinModelPortaliP59337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8183161.
STRINGi224911.blr1087.

Protein family/group databases

ESTHERibraja-dhaa. Haloalkane_dehalogenase-HLD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46352; BAC46352; BAC46352.
GeneIDi1051963.
KEGGibja:blr1087.
PATRICi21185702. VBIBraJap65052_1122.

Phylogenomic databases

eggNOGiENOG4108JSS. Bacteria.
ENOG410ZVH8. LUCA.
HOGENOMiHOG000051838.
InParanoidiP59337.
KOiK01563.
OrthoDBiEOG64FKGZ.
PhylomeDBiP59337.

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1096-MONOMER.
BRENDAi3.8.1.5. 929.

Miscellaneous databases

EvolutionaryTraceiP59337.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiDHAA_BRADU
AccessioniPrimary (citable) accession number: P59337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: February 22, 2003
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.