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Protein

Haloalkane dehalogenase

Gene

dhaA

Organism
Rhodococcus sp. (strain TDTM0003)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes primary, secondary and cyclic haloalkanes (chain length > C4).

Catalytic activityi

1-haloalkane + H2O = a primary alcohol + halide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41Halide1
Active sitei106Nucleophile1
Binding sitei107Halide1
Active sitei130Proton donor1
Active sitei272Proton acceptor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

ESTHERirhoso-halo1. Haloalkane_dehalogenase-HLD2.

Names & Taxonomyi

Protein namesi
Recommended name:
Haloalkane dehalogenase (EC:3.8.1.5)
Gene namesi
Name:dhaA
OrganismiRhodococcus sp. (strain TDTM0003)
Taxonomic identifieri269091 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002167771 – 294Haloalkane dehalogenaseAdd BLAST294

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 17Combined sources5
Beta strandi20 – 28Combined sources9
Beta strandi30 – 33Combined sources4
Beta strandi35 – 38Combined sources4
Helixi45 – 48Combined sources4
Turni49 – 51Combined sources3
Helixi52 – 55Combined sources4
Turni56 – 58Combined sources3
Beta strandi61 – 64Combined sources4
Helixi81 – 94Combined sources14
Beta strandi99 – 105Combined sources7
Helixi107 – 118Combined sources12
Helixi120 – 122Combined sources3
Beta strandi123 – 130Combined sources8
Helixi138 – 140Combined sources3
Helixi143 – 152Combined sources10
Helixi157 – 162Combined sources6
Helixi167 – 170Combined sources4
Helixi172 – 175Combined sources4
Beta strandi177 – 179Combined sources3
Helixi183 – 190Combined sources8
Helixi191 – 193Combined sources3
Helixi196 – 199Combined sources4
Helixi200 – 208Combined sources9
Helixi216 – 231Combined sources16
Beta strandi236 – 243Combined sources8
Beta strandi245 – 247Combined sources3
Helixi249 – 258Combined sources10
Beta strandi262 – 272Combined sources11
Helixi274 – 276Combined sources3
Helixi279 – 289Combined sources11
Helixi290 – 293Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BN6X-ray1.50A1-293[»]
1BN7X-ray1.50A1-293[»]
1CQWX-ray1.50A4-293[»]
ProteinModelPortaliP59336.
SMRiP59336.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59336.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 158AB hydrolase-1Sequence analysisAdd BLAST125

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P59336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEIGTGFPF DPHYVEVLGE RMHYVDVGPR DGTPVLFLHG NPTSSYLWRN
60 70 80 90 100
IIPHVAPSHR CIAPDLIGMG KSDKPDLDYF FDDHVRYLDA FIEALGLEEV
110 120 130 140 150
VLVIHDWGSA LGFHWAKRNP ERVKGIACME FIRPIPTWDE WPEFARETFQ
160 170 180 190 200
AFRTADVGRE LIIDQNAFIE GVLPKCVVRP LTEVEMDHYR EPFLKPVDRE
210 220 230 240 250
PLWRFPNEIP IAGEPANIVA LVEAYMNWLH QSPVPKLLFW GTPGVLIPPA
260 270 280 290
EAARLAESLP NCKTVDIGPG LHYLQEDNPD LIGSEIARWL PGLA
Length:294
Mass (Da):33,331
Last modified:February 22, 2003 - v1
Checksum:i190E6B9944E5DBEF
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BN6X-ray1.50A1-293[»]
1BN7X-ray1.50A1-293[»]
1CQWX-ray1.50A4-293[»]
ProteinModelPortaliP59336.
SMRiP59336.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERirhoso-halo1. Haloalkane_dehalogenase-HLD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP59336.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHAA_RHOSD
AccessioniPrimary (citable) accession number: P59336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: February 22, 2003
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.