P59330 (DCYD_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-cysteine desulfhydrase
EC=4.4.1.15

Gene names

Name:	dcyD
Ordered Locus Names:	SF1962, S2058

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine By similarity. HAMAP-Rule MF_01045
Catalytic activity	D-cysteine + H2O = H2S + NH3 + pyruvate. HAMAP-Rule MF_01045
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01045
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01045
Sequence similarities	Belongs to the ACC deaminase/D-cysteine desulfhydrase family.
Sequence caution	The sequence AAN43513.1 differs from that shown. Reason: Erroneous initiation. The sequence AAP17343.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-amino acid metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	D-cysteine desulfhydrase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 328	327	D-cysteine desulfhydrase HAMAP-Rule MF_01045		PRO_0000184518
Amino acid modifications
	Modified residue	51	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59330 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2CB80214A65497D8	FASTA	328	35,167
	10 20 30 40 50 60 MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD EVTPMAMGGN KLRKLEFLAA 70 80 90 100 110 120 DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE NPIGTTAENY LTNGNRLLLD 130 140 150 160 170 180 LFNTQIEMCD ALTDPNAQLE ELATRVEAQG FRPYVIPVGG SNALGALGYV ESALEIAQQC 190 200 210 220 230 240 EGAVNISSVV VASGSAGTHA GLAVGLEHLM PESELIGVTV SRSVADQLPK VVNLQQAIAK 250 260 270 280 290 300 ELELTASAEI LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS 310 320 QKRFKDEGPI LFIHTGGAPA LFAYHPHV

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN43513.1. Different initiation. AE014073 Genomic DNA. Translation: AAP17343.1. Different initiation.
RefSeq	NP_707806.3. NC_004337.2. NP_837534.2. NC_004741.1.
3D structure databases
ProteinModelPortal	P59330.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF1962.
Proteomic databases
PaxDb	P59330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN43513; AAN43513; SF1962. AAP17343; AAP17343; S2058.
GeneID	1025173. 1078375.
KEGG	sfl:SF1962. sfx:S2058.
PATRIC	18705590. VBIShiFle31049_2283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2515.
HOGENOM	HOG000022459.
KO	K05396.
ProtClustDB	PRK03910.
Family and domain databases
HAMAP	MF_01045. D-Cys_desulfhydr.
InterPro	IPR027278. ACCD_DCysDesulf. IPR005966. D-Cys_desShydrase. IPR023702. D_Cys_desulphydr_bac. IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF006278. ACCD_DCysDesulf. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01275. ACC_deam_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCYD_SHIFL

Accession

Primary (citable) accession number: P59330

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program