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Protein

WD repeat and HMG-box DNA-binding protein 1

Gene

Wdhd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1004 – 107370HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • RNA binding Source: MGI
  • RNA polymerase binding Source: MGI

GO - Biological processi

  • heterochromatin maintenance Source: MGI
  • regulation of chromosome organization Source: MGI
  • RNA processing Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and HMG-box DNA-binding protein 1
Alternative name(s):
Acidic nucleoplasmic DNA-binding protein 1
Short name:
And-1
Gene namesi
Name:Wdhd1
Synonyms:And1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2443514. Wdhd1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117WD repeat and HMG-box DNA-binding protein 1PRO_0000051339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei377 – 3771PhosphoserineCombined sources
Modified residuei664 – 6641N6-acetyllysineCombined sources
Modified residuei819 – 8191PhosphothreonineCombined sources
Modified residuei821 – 8211PhosphoserineCombined sources
Modified residuei910 – 9101PhosphoserineBy similarity
Modified residuei923 – 9231PhosphoserineBy similarity
Modified residuei953 – 9531N6-acetyllysineBy similarity
Modified residuei1030 – 10301PhosphoserineBy similarity
Cross-linki1116 – 1116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki1116 – 1116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP59328.
MaxQBiP59328.
PaxDbiP59328.
PeptideAtlasiP59328.
PRIDEiP59328.

PTM databases

iPTMnetiP59328.
PhosphoSiteiP59328.

Expressioni

Gene expression databases

BgeeiP59328.
CleanExiMM_WDHD1.
ExpressionAtlasiP59328. baseline and differential.
GenevisibleiP59328. MM.

Interactioni

Subunit structurei

Interacts with the polymerase alpha catalytic subunit POLA1. Interacts with MCM10. Interacts with DNA2 (By similarity).By similarity

GO - Molecular functioni

  • RNA polymerase binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107421.

Structurei

3D structure databases

ProteinModelPortaliP59328.
SMRiP59328. Positions 1006-1073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 5040WD 1Add
BLAST
Repeati52 – 9140WD 2Add
BLAST
Repeati93 – 13139WD 3Add
BLAST
Repeati134 – 17340WD 4Add
BLAST
Repeati184 – 22340WD 5Add
BLAST
Repeati228 – 26740WD 6Add
BLAST
Repeati271 – 31040WD 7Add
BLAST

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1274. Eukaryota.
ENOG410XSJV. LUCA.
GeneTreeiENSGT00390000002030.
HOGENOMiHOG000015160.
HOVERGENiHBG074190.
InParanoidiP59328.
KOiK11274.
OMAiMLKTGSS.
PhylomeDBiP59328.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR009071. HMG_box_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00505. HMG_box. 1 hit.
PF12341. Mcl1_mid. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P59328-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPATQKPMRY GHTEGHTEVC FDDSGSYIVT CGSDGDVRMW EDLDDDDPKS
60 70 80 90 100
VNVGEKAFSC ALKNGKLVTA VSNNTVQVYT FPEGVPDGIL TRFTTNANHV
110 120 130 140 150
VFNGAGNKIA AGSSDFLVKV VDVMDNSQQQ TFRGHDAPVL SLSFDPKDIF
160 170 180 190 200
LASASCDGTV RVWNISDQTC AVSWPVLQKS NDVVNAKSIC RLAWQPKAGK
210 220 230 240 250
LLAVPVEKSV KLYRRETWSN PFDLSDSSIS QTLNIVTWSP CGQYLAAGAI
260 270 280 290 300
NGLIVVWNVE TKDCMERVKH EKGYAICGLA WHPTCSRICY TDVEGNLGVL
310 320 330 340 350
ENVCDLSGKV SSNKVSSRVE KDYNDLFDGD DTSSAGDFLN DNAVEIPSFS
360 370 380 390 400
KGIINEDDDN DDIMLAADHD LGDDENSVDV TMLKADLSHK EEGDDDQARS
410 420 430 440 450
IHNLPLIRPQ RPFYDGPMPT PRQKPFQSSS TPLHLSHRFM VWNSVGIIRC
460 470 480 490 500
YNDDQDSAID VEFHDTSIHH ATHLLNAFNY TMGTLSHEAI LLACESADEL
510 520 530 540 550
ASKLHCLHFS SWDSSKEWMV DMPQNEDIEA ICLGLGWAAA ATTALLLRLF
560 570 580 590 600
TIGGVQKEVF CLPGPVVSMA GHGEQLCIVY HRGTGFDGDQ CLGVQLLELG
610 620 630 640 650
RKKNQVLHGD PLPLTRKSYL TWLGFSAEGT PCYVDSEGCV RMLNRGLGNT
660 670 680 690 700
WTPVCNIREH CKGKSDHYWV VGIHENPQQL RCIPCKGSRF PPTLPRPAVA
710 720 730 740 750
ILSFKLPYCQ TSTEKGQMEE QFWHSVLFHN YLDYLAKNGY DYEESIKNQA
760 770 780 790 800
VKEQQELLMK MLALSCKLER EFRCVELADL MTQNAVHLAI KYASRSRKLI
810 820 830 840 850
LAQKLSELAA EKAAELAETQ SEEEKEEDFR EKLNAGYSHT TTEWSRPRVR
860 870 880 890 900
SQVEDAEDRE DTVSEEKPES HNHGQNLFQS ANSSDTPALK SGAVFSSSQG
910 920 930 940 950
WVNPFKVVVS SKEPAVSANS TRSANILDSM NKSSRKSTSL NRMENNEKSP
960 970 980 990 1000
VIKPLTPKPR SKQASAASYF QKRTPQADKT EEVKENPKSS SSDAPAVCLQ
1010 1020 1030 1040 1050
NSENQRPKTG FQMWLEENRS QILSDNPDIS DETDIIKEGM IRFRVLSAEE
1060 1070 1080 1090 1100
RKAWTNKAKG ETASDGAEAK KRKRVVSEIC ETENQEETVK ENLDLSKKQK
1110
ALNLPANQKL SAFAFKQ
Length:1,117
Mass (Da):124,254
Last modified:January 10, 2006 - v2
Checksum:i01F80C3012B39EA7
GO
Isoform 2 (identifier: P59328-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-641: TPCYVDSEGCVR → KIIFFLYIKDIF
     642-1117: Missing.

Note: No experimental confirmation available.
Show »
Length:641
Mass (Da):70,637
Checksum:iC39755D52BE86DF0
GO
Isoform 3 (identifier: P59328-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-350: Missing.

Note: No experimental confirmation available.
Show »
Length:1,080
Mass (Da):120,217
Checksum:i07C387828084F8ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541G → V in BAC29408 (PubMed:16141072).Curated
Sequence conflicti350 – 3501S → P in BAC29408 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei314 – 35037Missing in isoform 3. 1 PublicationVSP_016898Add
BLAST
Alternative sequencei630 – 64112TPCYV…EGCVR → KIIFFLYIKDIF in isoform 2. 1 PublicationVSP_006757Add
BLAST
Alternative sequencei642 – 1117476Missing in isoform 2. 1 PublicationVSP_006758Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC063740 mRNA. Translation: AAH63740.1.
AK036390 mRNA. Translation: BAC29408.1.
AK052690 mRNA. Translation: BAC35097.1.
RefSeqiNP_766186.2. NM_172598.3.
UniGeneiMm.265615.

Genome annotation databases

EnsembliENSMUST00000111790; ENSMUSP00000107420; ENSMUSG00000037572. [P59328-2]
ENSMUST00000187531; ENSMUSP00000141182; ENSMUSG00000037572. [P59328-1]
GeneIDi218973.
KEGGimmu:218973.
UCSCiuc007thu.2. mouse. [P59328-3]
uc007thv.2. mouse. [P59328-1]
uc007thw.2. mouse. [P59328-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC063740 mRNA. Translation: AAH63740.1.
AK036390 mRNA. Translation: BAC29408.1.
AK052690 mRNA. Translation: BAC35097.1.
RefSeqiNP_766186.2. NM_172598.3.
UniGeneiMm.265615.

3D structure databases

ProteinModelPortaliP59328.
SMRiP59328. Positions 1006-1073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107421.

PTM databases

iPTMnetiP59328.
PhosphoSiteiP59328.

Proteomic databases

EPDiP59328.
MaxQBiP59328.
PaxDbiP59328.
PeptideAtlasiP59328.
PRIDEiP59328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111790; ENSMUSP00000107420; ENSMUSG00000037572. [P59328-2]
ENSMUST00000187531; ENSMUSP00000141182; ENSMUSG00000037572. [P59328-1]
GeneIDi218973.
KEGGimmu:218973.
UCSCiuc007thu.2. mouse. [P59328-3]
uc007thv.2. mouse. [P59328-1]
uc007thw.2. mouse. [P59328-2]

Organism-specific databases

CTDi11169.
MGIiMGI:2443514. Wdhd1.

Phylogenomic databases

eggNOGiKOG1274. Eukaryota.
ENOG410XSJV. LUCA.
GeneTreeiENSGT00390000002030.
HOGENOMiHOG000015160.
HOVERGENiHBG074190.
InParanoidiP59328.
KOiK11274.
OMAiMLKTGSS.
PhylomeDBiP59328.

Miscellaneous databases

PROiP59328.
SOURCEiSearch...

Gene expression databases

BgeeiP59328.
CleanExiMM_WDHD1.
ExpressionAtlasiP59328. baseline and differential.
GenevisibleiP59328. MM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR009071. HMG_box_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00505. HMG_box. 1 hit.
PF12341. Mcl1_mid. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-933 (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Bone and Kidney.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-821, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-819 AND SER-821, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiWDHD1_MOUSE
AccessioniPrimary (citable) accession number: P59328
Secondary accession number(s): Q6P408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: January 10, 2006
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.