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P59325

- IF5_MOUSE

UniProt

P59325 - IF5_MOUSE

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Protein

Eukaryotic translation initiation factor 5

Gene

Eif5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPSequence Analysis

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: Ensembl
  3. translation initiation factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_236458. Ribosomal scanning and start codon recognition.
REACT_262078. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:Eif5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:95309. Eif5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. plasma membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Eukaryotic translation initiation factor 5PRO_0000212517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei227 – 2271PhosphoserineBy similarity
Modified residuei387 – 3871Phosphoserine1 Publication
Modified residuei388 – 3881Phosphoserine1 Publication
Modified residuei417 – 4171Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP59325.
PaxDbiP59325.
PRIDEiP59325.

PTM databases

PhosphoSiteiP59325.

Expressioni

Gene expression databases

BgeeiP59325.
CleanExiMM_EIF5.
ExpressionAtlasiP59325. baseline and differential.
GenevestigatoriP59325.

Interactioni

Protein-protein interaction databases

IntActiP59325. 2 interactions.
MINTiMINT-1857485.

Structurei

3D structure databases

ProteinModelPortaliP59325.
SMRiP59325. Positions 3-150, 230-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 390160W2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi194 – 2007Asp/Glu-rich (highly acidic)
Compositional biasi382 – 40019Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi421 – 4277Asp-rich (acidic)

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1601.
HOGENOMiHOG000214327.
HOVERGENiHBG006132.
InParanoidiP59325.
KOiK03262.
OMAiMHQAQLL.
OrthoDBiEOG7FV3QF.
PhylomeDBiP59325.
TreeFamiTF101533.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59325-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP
60 70 80 90 100
TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP
110 120 130 140 150
ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD
160 170 180 190 200
IGTGKKEKEK KNRKGKDKEN GSVSTSETPP PPPPNEISPP HAVEEEEDDD
210 220 230 240 250
WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI LFDFVKKKKE
260 270 280 290 300
EGIIDSSDKE IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR
310 320 330 340 350
FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII
360 370 380 390 400
SWSEKASKKY VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE
410 420
VVYSKTASVP KVETVKSDNK DDDIDIDAI
Length:429
Mass (Da):48,968
Last modified:February 12, 2003 - v1
Checksum:iC77DD5DFBB5C1DEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA. Translation: AAH39275.1.
BC042622 mRNA. No translation available.
CCDSiCCDS26180.1.
RefSeqiNP_775539.1. NM_173363.5.
NP_829887.1. NM_178041.2.
XP_006542900.1. XM_006542837.1.
UniGeneiMm.271222.
Mm.470192.

Genome annotation databases

EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282.
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282.
GeneIDi102641436.
217869.
KEGGimmu:102641436.
mmu:217869.
UCSCiuc007pdd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA. Translation: AAH39275.1 .
BC042622 mRNA. No translation available.
CCDSi CCDS26180.1.
RefSeqi NP_775539.1. NM_173363.5.
NP_829887.1. NM_178041.2.
XP_006542900.1. XM_006542837.1.
UniGenei Mm.271222.
Mm.470192.

3D structure databases

ProteinModelPortali P59325.
SMRi P59325. Positions 3-150, 230-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P59325. 2 interactions.
MINTi MINT-1857485.

PTM databases

PhosphoSitei P59325.

Proteomic databases

MaxQBi P59325.
PaxDbi P59325.
PRIDEi P59325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050993 ; ENSMUSP00000061616 ; ENSMUSG00000021282 .
ENSMUST00000166123 ; ENSMUSP00000126825 ; ENSMUSG00000021282 .
GeneIDi 102641436.
217869.
KEGGi mmu:102641436.
mmu:217869.
UCSCi uc007pdd.2. mouse.

Organism-specific databases

CTDi 1983.
MGIi MGI:95309. Eif5.

Phylogenomic databases

eggNOGi COG1601.
HOGENOMi HOG000214327.
HOVERGENi HBG006132.
InParanoidi P59325.
KOi K03262.
OMAi MHQAQLL.
OrthoDBi EOG7FV3QF.
PhylomeDBi P59325.
TreeFami TF101533.

Enzyme and pathway databases

Reactomei REACT_236458. Ribosomal scanning and start codon recognition.
REACT_262078. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

ChiTaRSi Eif5. mouse.
NextBioi 376075.
PROi P59325.
SOURCEi Search...

Gene expression databases

Bgeei P59325.
CleanExi MM_EIF5.
ExpressionAtlasi P59325. baseline and differential.
Genevestigatori P59325.

Family and domain databases

Gene3Di 1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view ]
Pfami PF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view ]
SMARTi SM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view ]
SUPFAMi SSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEi PS51363. W2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Colon and Eye.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388 AND SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIF5_MOUSE
AccessioniPrimary (citable) accession number: P59325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3