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P59325

- IF5_MOUSE

UniProt

P59325 - IF5_MOUSE

Protein

Eukaryotic translation initiation factor 5

Gene

Eif5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (12 Feb 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 348GTPSequence Analysis

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. translation initiation factor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 5
    Short name:
    eIF-5
    Gene namesi
    Name:Eif5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:95309. Eif5.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 429429Eukaryotic translation initiation factor 5PRO_0000212517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei10 – 101PhosphoserineBy similarity
    Modified residuei227 – 2271PhosphoserineBy similarity
    Modified residuei387 – 3871Phosphoserine1 Publication
    Modified residuei388 – 3881Phosphoserine1 Publication
    Modified residuei417 – 4171Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP59325.
    PaxDbiP59325.
    PRIDEiP59325.

    PTM databases

    PhosphoSiteiP59325.

    Expressioni

    Gene expression databases

    ArrayExpressiP59325.
    BgeeiP59325.
    CleanExiMM_EIF5.
    GenevestigatoriP59325.

    Interactioni

    Protein-protein interaction databases

    IntActiP59325. 2 interactions.
    MINTiMINT-1857485.

    Structurei

    3D structure databases

    ProteinModelPortaliP59325.
    SMRiP59325. Positions 3-150, 230-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini231 – 390160W2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi194 – 2007Asp/Glu-rich (highly acidic)
    Compositional biasi382 – 40019Asp/Glu-rich (highly acidic)Add
    BLAST
    Compositional biasi421 – 4277Asp-rich (acidic)

    Sequence similaritiesi

    Belongs to the eIF-2-beta/eIF-5 family.Curated
    Contains 1 W2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1601.
    HOGENOMiHOG000214327.
    HOVERGENiHBG006132.
    InParanoidiP59325.
    KOiK03262.
    OMAiMHQAQLL.
    OrthoDBiEOG7FV3QF.
    PhylomeDBiP59325.
    TreeFamiTF101533.

    Family and domain databases

    Gene3Di1.25.40.180. 1 hit.
    3.30.30.50. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR002735. Transl_init_fac_IF2/IF5.
    IPR016189. Transl_init_fac_IF2/IF5_N.
    IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
    IPR003307. W2_domain.
    [Graphical view]
    PfamiPF01873. eIF-5_eIF-2B. 1 hit.
    PF02020. W2. 1 hit.
    [Graphical view]
    SMARTiSM00653. eIF2B_5. 1 hit.
    SM00515. eIF5C. 1 hit.
    [Graphical view]
    SUPFAMiSSF100966. SSF100966. 1 hit.
    SSF48371. SSF48371. 1 hit.
    SSF75689. SSF75689. 1 hit.
    PROSITEiPS51363. W2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59325-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP    50
    TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP 100
    ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD 150
    IGTGKKEKEK KNRKGKDKEN GSVSTSETPP PPPPNEISPP HAVEEEEDDD 200
    WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI LFDFVKKKKE 250
    EGIIDSSDKE IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR 300
    FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII 350
    SWSEKASKKY VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE 400
    VVYSKTASVP KVETVKSDNK DDDIDIDAI 429
    Length:429
    Mass (Da):48,968
    Last modified:February 12, 2003 - v1
    Checksum:iC77DD5DFBB5C1DEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC039275 mRNA. Translation: AAH39275.1.
    BC042622 mRNA. No translation available.
    CCDSiCCDS26180.1.
    RefSeqiNP_775539.1. NM_173363.5.
    NP_829887.1. NM_178041.2.
    XP_006542900.1. XM_006542837.1.
    UniGeneiMm.271222.
    Mm.470192.

    Genome annotation databases

    EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282.
    ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282.
    GeneIDi102641436.
    217869.
    KEGGimmu:102641436.
    mmu:217869.
    UCSCiuc007pdd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC039275 mRNA. Translation: AAH39275.1 .
    BC042622 mRNA. No translation available.
    CCDSi CCDS26180.1.
    RefSeqi NP_775539.1. NM_173363.5.
    NP_829887.1. NM_178041.2.
    XP_006542900.1. XM_006542837.1.
    UniGenei Mm.271222.
    Mm.470192.

    3D structure databases

    ProteinModelPortali P59325.
    SMRi P59325. Positions 3-150, 230-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P59325. 2 interactions.
    MINTi MINT-1857485.

    PTM databases

    PhosphoSitei P59325.

    Proteomic databases

    MaxQBi P59325.
    PaxDbi P59325.
    PRIDEi P59325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050993 ; ENSMUSP00000061616 ; ENSMUSG00000021282 .
    ENSMUST00000166123 ; ENSMUSP00000126825 ; ENSMUSG00000021282 .
    GeneIDi 102641436.
    217869.
    KEGGi mmu:102641436.
    mmu:217869.
    UCSCi uc007pdd.2. mouse.

    Organism-specific databases

    CTDi 1983.
    MGIi MGI:95309. Eif5.

    Phylogenomic databases

    eggNOGi COG1601.
    HOGENOMi HOG000214327.
    HOVERGENi HBG006132.
    InParanoidi P59325.
    KOi K03262.
    OMAi MHQAQLL.
    OrthoDBi EOG7FV3QF.
    PhylomeDBi P59325.
    TreeFami TF101533.

    Miscellaneous databases

    ChiTaRSi eIF5. mouse.
    NextBioi 376075.
    PROi P59325.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P59325.
    Bgeei P59325.
    CleanExi MM_EIF5.
    Genevestigatori P59325.

    Family and domain databases

    Gene3Di 1.25.40.180. 1 hit.
    3.30.30.50. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR002735. Transl_init_fac_IF2/IF5.
    IPR016189. Transl_init_fac_IF2/IF5_N.
    IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
    IPR003307. W2_domain.
    [Graphical view ]
    Pfami PF01873. eIF-5_eIF-2B. 1 hit.
    PF02020. W2. 1 hit.
    [Graphical view ]
    SMARTi SM00653. eIF2B_5. 1 hit.
    SM00515. eIF5C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF100966. SSF100966. 1 hit.
    SSF48371. SSF48371. 1 hit.
    SSF75689. SSF75689. 1 hit.
    PROSITEi PS51363. W2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Colon and Eye.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388 AND SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiIF5_MOUSE
    AccessioniPrimary (citable) accession number: P59325
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 12, 2003
    Last sequence update: February 12, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3