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P59325 (IF5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 5

Short name=eIF-5
Gene names
Name:Eif5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Sequence similarities

Belongs to the eIF-2-beta/eIF-5 family.

Contains 1 W2 domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Eukaryotic translation initiation factor 5
PRO_0000212517

Regions

Domain231 – 390160W2
Nucleotide binding27 – 348GTP Potential
Compositional bias194 – 2007Asp/Glu-rich (highly acidic)
Compositional bias382 – 40019Asp/Glu-rich (highly acidic)
Compositional bias421 – 4277Asp-rich (acidic)

Amino acid modifications

Modified residue101Phosphoserine By similarity
Modified residue2271Phosphoserine By similarity
Modified residue3871Phosphoserine Ref.3
Modified residue3881Phosphoserine Ref.3
Modified residue4171Phosphoserine Ref.3

Sequences

Sequence LengthMass (Da)Tools
P59325 [UniParc].

Last modified February 12, 2003. Version 1.
Checksum: C77DD5DFBB5C1DEF

FASTA42948,968
        10         20         30         40         50         60 
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE 

        70         80         90        100        110        120 
LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP ECENPETDLH VNPKKQTIGN 

       130        140        150        160        170        180 
SCKACGYRGM LDTHHKLCTF ILKNPPENSD IGTGKKEKEK KNRKGKDKEN GSVSTSETPP 

       190        200        210        220        230        240 
PPPPNEISPP HAVEEEEDDD WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI 

       250        260        270        280        290        300 
LFDFVKKKKE EGIIDSSDKE IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR 

       310        320        330        340        350        360 
FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII SWSEKASKKY 

       370        380        390        400        410        420 
VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE VVYSKTASVP KVETVKSDNK 


DDDIDIDAI 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Colon and Eye.
[2]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388 AND SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC039275 mRNA. Translation: AAH39275.1.
BC042622 mRNA. No translation available.
RefSeqNP_775539.1. NM_173363.5.
NP_829887.1. NM_178041.2.
XP_006542900.1. XM_006542837.1.
UniGeneMm.271222.
Mm.470192.

3D structure databases

ProteinModelPortalP59325.
SMRP59325. Positions 3-150, 230-405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP59325. 2 interactions.
MINTMINT-1857485.

PTM databases

PhosphoSiteP59325.

Proteomic databases

PaxDbP59325.
PRIDEP59325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282.
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282.
GeneID102641436.
217869.
KEGGmmu:100047658.
mmu:217869.
UCSCuc007pdd.2. mouse.

Organism-specific databases

CTD1983.
MGIMGI:95309. Eif5.

Phylogenomic databases

eggNOGCOG1601.
HOGENOMHOG000214327.
HOVERGENHBG006132.
InParanoidP59325.
KOK03262.
OMAKILQLYY.
OrthoDBEOG7FV3QF.
PhylomeDBP59325.
TreeFamTF101533.

Gene expression databases

ArrayExpressP59325.
BgeeP59325.
CleanExMM_EIF5.
GenevestigatorP59325.

Family and domain databases

Gene3D1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEPS51363. W2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSeIF5. mouse.
NextBio376075.
PROP59325.
SOURCESearch...

Entry information

Entry nameIF5_MOUSE
AccessionPrimary (citable) accession number: P59325
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Translation initiation factors

List of translation initiation factor entries