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Protein

Eukaryotic translation initiation factor 5

Gene

Eif5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_292264. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_305839. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:Eif5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:95309. Eif5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Eukaryotic translation initiation factor 5PRO_0000212517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei227 – 2271PhosphoserineBy similarity
Modified residuei387 – 3871Phosphoserine1 Publication
Modified residuei388 – 3881Phosphoserine1 Publication
Modified residuei417 – 4171Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP59325.
PaxDbiP59325.
PRIDEiP59325.

PTM databases

PhosphoSiteiP59325.

Expressioni

Gene expression databases

BgeeiP59325.
CleanExiMM_EIF5.
ExpressionAtlasiP59325. baseline and differential.
GenevisibleiP59325. MM.

Interactioni

Protein-protein interaction databases

IntActiP59325. 3 interactions.
MINTiMINT-1857485.
STRINGi10090.ENSMUSP00000061616.

Structurei

3D structure databases

ProteinModelPortaliP59325.
SMRiP59325. Positions 3-150, 230-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 390160W2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi194 – 2007Asp/Glu-rich (highly acidic)
Compositional biasi382 – 40019Asp/Glu-rich (highly acidic)Add
BLAST
Compositional biasi421 – 4277Asp-rich (acidic)

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1601.
GeneTreeiENSGT00390000016478.
HOGENOMiHOG000214327.
HOVERGENiHBG006132.
InParanoidiP59325.
KOiK03262.
OMAiMHQAQLL.
OrthoDBiEOG7FV3QF.
PhylomeDBiP59325.
TreeFamiTF101533.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP
60 70 80 90 100
TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDMLD GFIKKFVLCP
110 120 130 140 150
ECENPETDLH VNPKKQTIGN SCKACGYRGM LDTHHKLCTF ILKNPPENSD
160 170 180 190 200
IGTGKKEKEK KNRKGKDKEN GSVSTSETPP PPPPNEISPP HAVEEEEDDD
210 220 230 240 250
WGEDTTEEAQ RRRMDEISDH AKGLTLSDDL ERTVEERVNI LFDFVKKKKE
260 270 280 290 300
EGIIDSSDKE IVAEAERLDV KAMGPLVLTE VLFDEKIREQ IKKYRRHFLR
310 320 330 340 350
FCHNNKKAQR YLLHGLECVV AMHQAQLISK IPHILKEMYD ADLLEEEVII
360 370 380 390 400
SWSEKASKKY VSKELAKEIR VKAEPFIKWL KEAEEESSGG EEEDEDENIE
410 420
VVYSKTASVP KVETVKSDNK DDDIDIDAI
Length:429
Mass (Da):48,968
Last modified:February 12, 2003 - v1
Checksum:iC77DD5DFBB5C1DEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA. Translation: AAH39275.1.
BC042622 mRNA. No translation available.
CCDSiCCDS26180.1.
RefSeqiNP_775539.1. NM_173363.5.
NP_829887.1. NM_178041.2.
XP_006542900.1. XM_006542837.2.
UniGeneiMm.271222.
Mm.470192.

Genome annotation databases

EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282.
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282.
GeneIDi102641436.
217869.
KEGGimmu:102641436.
mmu:217869.
UCSCiuc007pdd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039275 mRNA. Translation: AAH39275.1.
BC042622 mRNA. No translation available.
CCDSiCCDS26180.1.
RefSeqiNP_775539.1. NM_173363.5.
NP_829887.1. NM_178041.2.
XP_006542900.1. XM_006542837.2.
UniGeneiMm.271222.
Mm.470192.

3D structure databases

ProteinModelPortaliP59325.
SMRiP59325. Positions 3-150, 230-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP59325. 3 interactions.
MINTiMINT-1857485.
STRINGi10090.ENSMUSP00000061616.

PTM databases

PhosphoSiteiP59325.

Proteomic databases

MaxQBiP59325.
PaxDbiP59325.
PRIDEiP59325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050993; ENSMUSP00000061616; ENSMUSG00000021282.
ENSMUST00000166123; ENSMUSP00000126825; ENSMUSG00000021282.
GeneIDi102641436.
217869.
KEGGimmu:102641436.
mmu:217869.
UCSCiuc007pdd.2. mouse.

Organism-specific databases

CTDi1983.
MGIiMGI:95309. Eif5.

Phylogenomic databases

eggNOGiCOG1601.
GeneTreeiENSGT00390000016478.
HOGENOMiHOG000214327.
HOVERGENiHBG006132.
InParanoidiP59325.
KOiK03262.
OMAiMHQAQLL.
OrthoDBiEOG7FV3QF.
PhylomeDBiP59325.
TreeFamiTF101533.

Enzyme and pathway databases

ReactomeiREACT_292264. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_305839. Ribosomal scanning and start codon recognition.

Miscellaneous databases

ChiTaRSiEif5. mouse.
NextBioi376075.
PROiP59325.
SOURCEiSearch...

Gene expression databases

BgeeiP59325.
CleanExiMM_EIF5.
ExpressionAtlasiP59325. baseline and differential.
GenevisibleiP59325. MM.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
3.30.30.50. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
IPR003307. W2_domain.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
SM00515. eIF5C. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF48371. SSF48371. 1 hit.
SSF75689. SSF75689. 1 hit.
PROSITEiPS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Colon and Eye.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388 AND SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIF5_MOUSE
AccessioniPrimary (citable) accession number: P59325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: July 22, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.