P59295 (ARGB_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BL1062

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence AAN24870.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112591
Regions
	Region	67 – 68	2	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Site	32	1	Transition state stabilizer By similarity
	Site	251	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59295 [UniParc]. Last modified November 21, 2003. Version 3. Checksum: 5C60A2B549458FFC	FASTA	305	32,402
	10 20 30 40 50 60 MHTDLRADQK AEVLIEALPW LEEFAGQRIV VKYGGNAMVD DHLKQCFAED MVFLRQVGLH 70 80 90 100 110 120 PIVVHGGGPQ ISHMLKALGI KSEFKGGLRV TTPEAMDVVR MVLTGKVSRE LVGLINAHGP 130 140 150 160 170 180 LAVGMSGEDG GLFSAMQRRP VIDGKPTDIG LVGDVVSVDA SAVEDLVAAG RIPVVSSVAP 190 200 210 220 230 240 NEEDATEVLN VNADSAAAAL AAAVGAHKLV ILTDVDGLYA DWPDKNSLIG RIGVENLRDM 250 260 270 280 290 300 LPDLESGMRP KMEACVRAID GGVPQAHVID GRKPHSILNE IFTSAGIGTM VMPDEGLEMR SSYGY

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24870.1. Different initiation.
RefSeq	NP_696234.1. NC_004307.2.
3D structure databases
ProteinModelPortal	P59295.
SMR	P59295. Positions 10-296.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022594.
GenomeReviews	Gene locus BL1062 in contig AE014295_GR.
KEGG	blo:BL1062.
NMPDR	fig|206672.1.peg.1001.
PATRIC	21118609. VBIBifLon107831_0769.
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	P59295.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BLON206672:BL1062-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BIFLO

Accession

Primary (citable) accession number: P59295

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 21, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program