Reviewed, UniProtKB/Swiss-Prot P59293 (ARGA_SHIFL)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	SF2829, S3026

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Enzyme regulation	Feedback inhibition by L-arginine By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subunit structure	Homohexamer Probable.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186807
Regions
	Domain	296 – 443	148	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P59293-1 [UniParc]. Last modified February 12, 2003. Version 1. Checksum: 6A3C3EDF90170B47	FASTA	443	49,209
	10 20 30 40 50 60 MVKERKTELV EGFRHSVPYI NTHRGKTFVI MLGGEAIEHE NFSSIVNDIG LLHSLGIRLV 70 80 90 100 110 120 VVYGARPQID ANLAAHHHEP LYHKNIRVTD AKTLELVKQA AGTLQLDITA RLSMSLNNTP 130 140 150 160 170 180 LQGAHINVVS GNFIIAQPLG VDDGVDYCHS GRIRRIDEDA LHRQLESGAI VLMGPVAVSV 190 200 210 220 230 240 TGESFNLTSE EIATQLAIKL KAEKMIGFCS SQGVTNDDGD IVSELFPNEA QARVEAQEEK 250 260 270 280 290 300 GDYNSGTVRF LRGAVKACRS GVRRCHLISY QEDGALLQEL FSRDGIGTQI VMESAEQIRR 310 320 330 340 350 360 ATINDIGGIL ELIRPLEQQG ILVRRSREQL EMEIDKFTII QRDNTTIACA ALYPFPEEKI 370 380 390 400 410 420 GEMACVAVHP DYRSSSRGEV LLERIAAQAK QSGLSKLFVL TTRSIHWFQE RGFTPVDIDL 430 440 LPESKKQLYN YQRKSKVLMA DLG

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44316.1. Different initiation. AE014073 Genomic DNA. Translation: AAP18141.1.
RefSeq	NP_708609.2. NP_838331.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1025049. 1079294.
GenomeReviews	Gene locus SF2829 in contig AE005674_GR. Gene locus S3026 in contig AE014073_GR.
KEGG	sfl:SF2829. sfx:S3026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59293.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44316.1-MON.
BRENDA	2.3.1.1. 189495.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_SHIFL

Accession

Primary (citable) accession number: P59293

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	February 12, 2003
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)