Reviewed, UniProtKB/Swiss-Prot P59292 (ARGA_SHEON)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	SO_4245

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm Probable.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (argJ), argA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186806
Regions
	Domain	299 – 445	147	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P59292-1 [UniParc]. Last modified February 12, 2003. Version 1. Checksum: 6A0DD4F96A135C67	FASTA	445	48,937
	10 20 30 40 50 60 MRCKQRVRTT ELVDGFRHSA PYVNAHRGKT FVVMLGGEAL AQNQFRGILN DVALLHSLGI 70 80 90 100 110 120 KVVLVYGARP QIDAALAANG IEPAYHDGVR ITDEDSLKVI KQVAGALQFD ITARLSMSLS 130 140 150 160 170 180 NTPMQGAQIN LVSGNFVIAQ PLGVDNGVDF CLSGKVRRID AQGLKRQLDN HCIVLMGPIA 190 200 210 220 230 240 ASVTGESFNL TAEEIATQVA IKLKADKMIG FSSQNGILDR NGDVIAELMP NDAQKILNKL 250 260 270 280 290 300 AEQGSACIGT MAFLKASIDA CRNGVPRCHL VSYLDDGALL QELFSREGIG TQIVTESAER 310 320 330 340 350 360 LRRASISDIG GILNLIRPLE EQGILVRRSR EQLEIEIEQF MLIERDGLVI GCAALYPFEE 370 380 390 400 410 420 DNAGEFACLV VHPDYRDADR GSLLLKNIIG QARSRGYSRL FALTTRSIHW FLEHGFVIED 430 440 VDALPQKKKQ LYNYQRRSKI LALDL

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN57216.1.
RefSeq	NP_719772.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1171849.
GenomeReviews	Gene locus SO_4245 in contig AE014299_GR.
KEGG	son:SO_4245.
NMPDR	fig|211586.1.peg.3847.
TIGR	SO_4245.
Phylogenomic databases
HOGENOM	P59292.
OMA	DLIRPLE.
Enzyme and pathway databases
BioCyc	SONE211586:SO_4245-MON.
BRENDA	2.3.1.1. 257422.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_SHEON

Accession

Primary (citable) accession number: P59292

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	February 12, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)